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The ability of human electrochemiluminescence immunoassay to measure testosterone and progesterone in ovine plasma

The present study describes the use of electrochemiluminescence immunoassay (ECL) with the specific human kit for measuring plasma testosterone and progesterone in ovine. This study was carried out on 16 mixed age animals of the local breed. They were divided into two groups of both sexes, namely Ma...

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Main Authors: Ayad, Abdelhanine, Benhanifia, Mokhtar, Benbarek, Hama
Format: Journal Contribution
Language:English
Published: 2020
Subjects:
Sheep
Immunoassay
Testosterone
Plasma progesterone
Endocrine glands
Gestation
Sexual reproduction
Reproductive efficiency
Reproductive ability
Reproductive performance
Testicular diseases
Animal pathology
Online Access:http://agris.upm.edu.my:8080/dspace/handle/0/18005
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spelling oai:http:--agris.upm.edu.my:0-18005The ability of human electrochemiluminescence immunoassay to measure testosterone and progesterone in ovine plasmaAyad, AbdelhanineBenhanifia, MokhtarBenbarek, HamaSheepImmunoassayTestosteronePlasma progesteroneEndocrine glandsGestationSexual reproductionReproductive efficiencyReproductive abilityReproductive performanceTesticular diseasesAnimal pathologyThe present study describes the use of electrochemiluminescence immunoassay (ECL) with the specific human kit for measuring plasma testosterone and progesterone in ovine. This study was carried out on 16 mixed age animals of the local breed. They were divided into two groups of both sexes, namely Male-1 Group, Male-2 Group, Female-1 Group and Female-2 Group. Blood samples were collected from the jugular vein into tubes containing EDTA and centrifuged at 1500 rpm for 20 min. Plasma was rapidly separated and stored at -20° C until assayed. The reproducibility inter- and intra-assay of the P4- and T-ECL is satisfactory (2.11-7.3 %). The accuracy (93-102 %) and the test of parallelism were largely acceptable. No cross-reaction was observed with the different hormones including PMSG,hCG, progesterone,testosterone, oxytocin and PGF2α when concentrations of 10 UI/ml and 10-3 UI/ml were used. T concentrations were higher in Male-1 Group than in Male2 Group (1.67 ± 1.15 and 0.38 ± 0.45 ng/ml, respectively). The female-1 Group showed very high P4 concentrations (15.17 ± 4.91 ng/ ml) compared to the Female-2 Group (0.08 ± 0.04 ng/ml). The results obtained clearly show that human ECL system can be used to measure progesterone and testosterone in plasma ovine2020-11-19T09:19:44Z2020-11-19T09:19:44Z2018Journal ContributionArticleNon-RefereedMalaysian Journal of Veterinary Research, 9 (1), p. 22-302180-3897http://agris.upm.edu.my:8080/dspace/handle/0/18005MY2020051311enhttp://www.dvs.gov.my/dvs/resources/user_16/MJVR%20Vol9%20No%201/MJVR-V9N1-p22-30.pdfAlgeriahttp://www.oceandocs.org/license
institution AGRIS
collection AGRIS
language English
topic Sheep
Immunoassay
Testosterone
Plasma progesterone
Endocrine glands
Gestation
Sexual reproduction
Reproductive efficiency
Reproductive ability
Reproductive performance
Testicular diseases
Animal pathology
spellingShingle Sheep
Immunoassay
Testosterone
Plasma progesterone
Endocrine glands
Gestation
Sexual reproduction
Reproductive efficiency
Reproductive ability
Reproductive performance
Testicular diseases
Animal pathology
Ayad, Abdelhanine
Benhanifia, Mokhtar
Benbarek, Hama
The ability of human electrochemiluminescence immunoassay to measure testosterone and progesterone in ovine plasma
description The present study describes the use of electrochemiluminescence immunoassay (ECL) with the specific human kit for measuring plasma testosterone and progesterone in ovine. This study was carried out on 16 mixed age animals of the local breed. They were divided into two groups of both sexes, namely Male-1 Group, Male-2 Group, Female-1 Group and Female-2 Group. Blood samples were collected from the jugular vein into tubes containing EDTA and centrifuged at 1500 rpm for 20 min. Plasma was rapidly separated and stored at -20° C until assayed. The reproducibility inter- and intra-assay of the P4- and T-ECL is satisfactory (2.11-7.3 %). The accuracy (93-102 %) and the test of parallelism were largely acceptable. No cross-reaction was observed with the different hormones including PMSG,hCG, progesterone,testosterone, oxytocin and PGF2α when concentrations of 10 UI/ml and 10-3 UI/ml were used. T concentrations were higher in Male-1 Group than in Male2 Group (1.67 ± 1.15 and 0.38 ± 0.45 ng/ml, respectively). The female-1 Group showed very high P4 concentrations (15.17 ± 4.91 ng/ ml) compared to the Female-2 Group (0.08 ± 0.04 ng/ml). The results obtained clearly show that human ECL system can be used to measure progesterone and testosterone in plasma ovine
format Journal Contribution
author Ayad, Abdelhanine
Benhanifia, Mokhtar
Benbarek, Hama
author_facet Ayad, Abdelhanine
Benhanifia, Mokhtar
Benbarek, Hama
author_sort Ayad, Abdelhanine
title The ability of human electrochemiluminescence immunoassay to measure testosterone and progesterone in ovine plasma
title_short The ability of human electrochemiluminescence immunoassay to measure testosterone and progesterone in ovine plasma
title_full The ability of human electrochemiluminescence immunoassay to measure testosterone and progesterone in ovine plasma
title_fullStr The ability of human electrochemiluminescence immunoassay to measure testosterone and progesterone in ovine plasma
title_full_unstemmed The ability of human electrochemiluminescence immunoassay to measure testosterone and progesterone in ovine plasma
title_sort ability of human electrochemiluminescence immunoassay to measure testosterone and progesterone in ovine plasma
publishDate 2020
url http://agris.upm.edu.my:8080/dspace/handle/0/18005
_version_ 1819285378517958656
score 13.4562235

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