Staff View: PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India

PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India

A total of 16 non-O1/ non-O139 Vibrio cholerae isolates were obtained from ornamental fish collected from aquarium traders located in four metro cities of India. All the isolates were confirmed by amplifying the 300 bp fragment in the 16S-23S rRNA intergenic spacer regions. Amplification of 431 bp f...

Full description

Saved in:

Bibliographic Details
Main Authors:	Rathore, Gaurav, Sood, Neeraj, Abidi, Rehana, Lakra, W. S., Swaminathan, T. Raja
Format:	Journal Contribution
Language:	English
Published:	2021
Subjects:	Vibrio Vibrio cholerae Carassius auratus Goldfish Isolation Identification Cloning DNA DNA sequence Gel electrophoresis Nucleotides Nucleotide sequence Polymerase chain reaction Ribosomal rna
Online Access:	http://agris.upm.edu.my:8080/dspace/handle/0/18975
Tags:	Add Tag No Tags, Be the first to tag this record!

id	oai:http:--agris.upm.edu.my:0-18975
record_format	dspace
spelling	oai:http:--agris.upm.edu.my:0-18975PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in IndiaRathore, GauravSood, NeerajAbidi, RehanaLakra, W. S.Swaminathan, T. RajaVibrioVibrio choleraeCarassius auratusGoldfishIsolationIdentificationCloningDNADNA sequenceGel electrophoresisNucleotidesNucleotide sequencePolymerase chain reactionRibosomal rnaA total of 16 non-O1/ non-O139 Vibrio cholerae isolates were obtained from ornamental fish collected from aquarium traders located in four metro cities of India. All the isolates were confirmed by amplifying the 300 bp fragment in the 16S-23S rRNA intergenic spacer regions. Amplification of 431 bp fragment of gene, which resulted in detectable levels of PCR product, was achieved with a minimum of 8 CFU/ml of V. cholerae. The detection limit for rtxA gene by PCR amplification of genomic DNA was 20 picogram. A 431 bp fragment of the rtxA gene of V. cholerae was cloned and sequenced (NCBI accession number EU714289). Nucleotide sequence analysis data of the rtxA gene showed that about 96% identical to those of V. cholerae RTX toxin gene cluster.2021-06-23T08:28:44Z2021-06-23T08:28:44Z2009Journal ContributionArticleNon-RefereedAsian Fisheries Science Journal (Malaysia), 22 (2), p. 707-7120116-6514http://agris.upm.edu.my:8080/dspace/handle/0/18975MY2021050785enhttp://www.asianfisheriessociety.org/publication/downloadfile.php?id=1067&file=Y0dSbUx6QTROalV3T0Rnd01ERTBNemczTkRRMU5qSXVjR1JtIndiahttp://www.oceandocs.org/license
institution	AGRIS
collection	AGRIS
language	English
topic	Vibrio Vibrio cholerae Carassius auratus Goldfish Isolation Identification Cloning DNA DNA sequence Gel electrophoresis Nucleotides Nucleotide sequence Polymerase chain reaction Ribosomal rna
spellingShingle	Vibrio Vibrio cholerae Carassius auratus Goldfish Isolation Identification Cloning DNA DNA sequence Gel electrophoresis Nucleotides Nucleotide sequence Polymerase chain reaction Ribosomal rna Rathore, Gaurav Sood, Neeraj Abidi, Rehana Lakra, W. S. Swaminathan, T. Raja PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India
description	A total of 16 non-O1/ non-O139 Vibrio cholerae isolates were obtained from ornamental fish collected from aquarium traders located in four metro cities of India. All the isolates were confirmed by amplifying the 300 bp fragment in the 16S-23S rRNA intergenic spacer regions. Amplification of 431 bp fragment of gene, which resulted in detectable levels of PCR product, was achieved with a minimum of 8 CFU/ml of V. cholerae. The detection limit for rtxA gene by PCR amplification of genomic DNA was 20 picogram. A 431 bp fragment of the rtxA gene of V. cholerae was cloned and sequenced (NCBI accession number EU714289). Nucleotide sequence analysis data of the rtxA gene showed that about 96% identical to those of V. cholerae RTX toxin gene cluster.
format	Journal Contribution
author	Rathore, Gaurav Sood, Neeraj Abidi, Rehana Lakra, W. S. Swaminathan, T. Raja
author_facet	Rathore, Gaurav Sood, Neeraj Abidi, Rehana Lakra, W. S. Swaminathan, T. Raja
author_sort	Rathore, Gaurav
title	PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India
title_short	PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India
title_full	PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India
title_fullStr	PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India
title_full_unstemmed	PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India
title_sort	pcr cloning and partial sequencing of rtxa gene of non-o1 / non-o139 vibrio cholerae isolated from gold fish carassius auratus in india
publishDate	2021
url	http://agris.upm.edu.my:8080/dspace/handle/0/18975
_version_	1782772625677746176
score	12.935284

PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India

Similar Items