PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India

A total of 16 non-O1/ non-O139 Vibrio cholerae isolates were obtained from ornamental fish collected from aquarium traders located in four metro cities of India. All the isolates were confirmed by amplifying the 300 bp fragment in the 16S-23S rRNA intergenic spacer regions. Amplification of 431 bp f...

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Main Authors: Rathore, Gaurav, Sood, Neeraj, Abidi, Rehana, Lakra, W. S., Swaminathan, T. Raja
Format: Journal Contribution
Language:English
Published: 2021
Subjects:
DNA
Online Access:http://agris.upm.edu.my:8080/dspace/handle/0/18975
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spelling oai:http:--agris.upm.edu.my:0-18975PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in IndiaRathore, GauravSood, NeerajAbidi, RehanaLakra, W. S.Swaminathan, T. RajaVibrioVibrio choleraeCarassius auratusGoldfishIsolationIdentificationCloningDNADNA sequenceGel electrophoresisNucleotidesNucleotide sequencePolymerase chain reactionRibosomal rnaA total of 16 non-O1/ non-O139 Vibrio cholerae isolates were obtained from ornamental fish collected from aquarium traders located in four metro cities of India. All the isolates were confirmed by amplifying the 300 bp fragment in the 16S-23S rRNA intergenic spacer regions. Amplification of 431 bp fragment of gene, which resulted in detectable levels of PCR product, was achieved with a minimum of 8 CFU/ml of V. cholerae. The detection limit for rtxA gene by PCR amplification of genomic DNA was 20 picogram. A 431 bp fragment of the rtxA gene of V. cholerae was cloned and sequenced (NCBI accession number EU714289). Nucleotide sequence analysis data of the rtxA gene showed that about 96% identical to those of V. cholerae RTX toxin gene cluster.2021-06-23T08:28:44Z2021-06-23T08:28:44Z2009Journal ContributionArticleNon-RefereedAsian Fisheries Science Journal (Malaysia), 22 (2), p. 707-7120116-6514http://agris.upm.edu.my:8080/dspace/handle/0/18975MY2021050785enhttp://www.asianfisheriessociety.org/publication/downloadfile.php?id=1067&file=Y0dSbUx6QTROalV3T0Rnd01ERTBNemczTkRRMU5qSXVjR1JtIndiahttp://www.oceandocs.org/license
institution AGRIS
collection AGRIS
language English
topic Vibrio
Vibrio cholerae
Carassius auratus
Goldfish
Isolation
Identification
Cloning
DNA
DNA sequence
Gel electrophoresis
Nucleotides
Nucleotide sequence
Polymerase chain reaction
Ribosomal rna
spellingShingle Vibrio
Vibrio cholerae
Carassius auratus
Goldfish
Isolation
Identification
Cloning
DNA
DNA sequence
Gel electrophoresis
Nucleotides
Nucleotide sequence
Polymerase chain reaction
Ribosomal rna
Rathore, Gaurav
Sood, Neeraj
Abidi, Rehana
Lakra, W. S.
Swaminathan, T. Raja
PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India
description A total of 16 non-O1/ non-O139 Vibrio cholerae isolates were obtained from ornamental fish collected from aquarium traders located in four metro cities of India. All the isolates were confirmed by amplifying the 300 bp fragment in the 16S-23S rRNA intergenic spacer regions. Amplification of 431 bp fragment of gene, which resulted in detectable levels of PCR product, was achieved with a minimum of 8 CFU/ml of V. cholerae. The detection limit for rtxA gene by PCR amplification of genomic DNA was 20 picogram. A 431 bp fragment of the rtxA gene of V. cholerae was cloned and sequenced (NCBI accession number EU714289). Nucleotide sequence analysis data of the rtxA gene showed that about 96% identical to those of V. cholerae RTX toxin gene cluster.
format Journal Contribution
author Rathore, Gaurav
Sood, Neeraj
Abidi, Rehana
Lakra, W. S.
Swaminathan, T. Raja
author_facet Rathore, Gaurav
Sood, Neeraj
Abidi, Rehana
Lakra, W. S.
Swaminathan, T. Raja
author_sort Rathore, Gaurav
title PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India
title_short PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India
title_full PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India
title_fullStr PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India
title_full_unstemmed PCR cloning and partial sequencing of rtxA gene of non-O1 / non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India
title_sort pcr cloning and partial sequencing of rtxa gene of non-o1 / non-o139 vibrio cholerae isolated from gold fish carassius auratus in india
publishDate 2021
url http://agris.upm.edu.my:8080/dspace/handle/0/18975
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