Staff View: Phylogenetic tree construction in reconfirmation of parasitoid species (Braconidae: Opiinae), reared from fruit flies (Bactrocera papayae) infesting star fruit (Averrhoa carambola) based on mitochondrial 16S rRNA sequences

Phylogenetic tree construction in reconfirmation of parasitoid species (Braconidae: Opiinae), reared from fruit flies (Bactrocera papayae) infesting star fruit (Averrhoa carambola) based on mitochondrial 16S rRNA sequences

Opiinae (Hymenoptera: Braconidae) is an important parasitoid of fruit flies (Diptera: Tephritidae). Accurate identification is needed for them to be effectively used as biological control agents. This study demonstrates the use of molecular phylogenetic analyses to reconfirm the species of Opiinae r...

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Main Authors:	Suhana, Y., Shariff, S., Ibrahim, N. J., Yaakop, S, Idris, A. B., Md-Zain, B. M., Roff, M. N.
Format:	Journal Contribution
Language:	English
Published:	2015
Subjects:	Bactrocera Parasitoids Star fruit Hymenoptera Braconidae
Online Access:	http://agris.upm.edu.my:8080/dspace/handle/0/9825
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id	oai:http:--agris.upm.edu.my:0-9825
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spelling	oai:http:--agris.upm.edu.my:0-9825Phylogenetic tree construction in reconfirmation of parasitoid species (Braconidae: Opiinae), reared from fruit flies (Bactrocera papayae) infesting star fruit (Averrhoa carambola) based on mitochondrial 16S rRNA sequencesSuhana, Y.Shariff, S.Ibrahim, N. J.Yaakop, SIdris, A. B.Md-Zain, B. M.Roff, M. N.BactroceraParasitoidsStar fruitHymenopteraBraconidaeOpiinae (Hymenoptera: Braconidae) is an important parasitoid of fruit flies (Diptera: Tephritidae). Accurate identification is needed for them to be effectively used as biological control agents. This study demonstrates the use of molecular phylogenetic analyses to reconfirm the species of Opiinae reared from tephritids infesting star fruit species. A total of 14 specimens were isolated and a 401 bp of mitochondrial 16S rRNA gene was amplified and sequenced from each of them. Three individual tephritids hosts (Bactrocera papayae) were also identified based on the mitochondrial ND1 gene. Maximum Parsimony (MP) tree was constructed using PAUP 4.0b10. Three species of Opiinae were successfully identified based on the mitochondrial 16S rRNA gene, namely Diachasmimorpha longicaudata (Ashmead), Fopius arisanus (Sonan),and Psyttalia incisi(Silvestri).These molecular-based findings reconfirmed the parasitoid species ofB. papayaeas recorded by previous studies based on morphology. In fact, the identification of Opiinae individuals have been reconfirmed based on topology and branching pattern of the phylogenetic tree as well as based on genetic distance analyses, which matched morphological-based identification.2015-08-25T07:24:46Z2015-08-25T07:24:46Z2013Journal ContributionArticleNon-RefereedPertanika Journal of Tropical Agricultural Science (Malaysia), 36 (4), p. 345-3581511-3701http://agris.upm.edu.my:8080/dspace/handle/0/9825MY2015050486enhttp://www.pertanika2.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2036%20%284%29%20Nov.%202013/05%20Page%20345-358.pdfMalaysiahttp://www.oceandocs.org/license
institution	AGRIS
collection	AGRIS
language	English
topic	Bactrocera Parasitoids Star fruit Hymenoptera Braconidae
spellingShingle	Bactrocera Parasitoids Star fruit Hymenoptera Braconidae Suhana, Y. Shariff, S. Ibrahim, N. J. Yaakop, S Idris, A. B. Md-Zain, B. M. Roff, M. N. Phylogenetic tree construction in reconfirmation of parasitoid species (Braconidae: Opiinae), reared from fruit flies (Bactrocera papayae) infesting star fruit (Averrhoa carambola) based on mitochondrial 16S rRNA sequences
description	Opiinae (Hymenoptera: Braconidae) is an important parasitoid of fruit flies (Diptera: Tephritidae). Accurate identification is needed for them to be effectively used as biological control agents. This study demonstrates the use of molecular phylogenetic analyses to reconfirm the species of Opiinae reared from tephritids infesting star fruit species. A total of 14 specimens were isolated and a 401 bp of mitochondrial 16S rRNA gene was amplified and sequenced from each of them. Three individual tephritids hosts (Bactrocera papayae) were also identified based on the mitochondrial ND1 gene. Maximum Parsimony (MP) tree was constructed using PAUP 4.0b10. Three species of Opiinae were successfully identified based on the mitochondrial 16S rRNA gene, namely Diachasmimorpha longicaudata (Ashmead), Fopius arisanus (Sonan),and Psyttalia incisi(Silvestri).These molecular-based findings reconfirmed the parasitoid species ofB. papayaeas recorded by previous studies based on morphology. In fact, the identification of Opiinae individuals have been reconfirmed based on topology and branching pattern of the phylogenetic tree as well as based on genetic distance analyses, which matched morphological-based identification.
format	Journal Contribution
author	Suhana, Y. Shariff, S. Ibrahim, N. J. Yaakop, S Idris, A. B. Md-Zain, B. M. Roff, M. N.
author_facet	Suhana, Y. Shariff, S. Ibrahim, N. J. Yaakop, S Idris, A. B. Md-Zain, B. M. Roff, M. N.
author_sort	Suhana, Y.
title	Phylogenetic tree construction in reconfirmation of parasitoid species (Braconidae: Opiinae), reared from fruit flies (Bactrocera papayae) infesting star fruit (Averrhoa carambola) based on mitochondrial 16S rRNA sequences
title_short	Phylogenetic tree construction in reconfirmation of parasitoid species (Braconidae: Opiinae), reared from fruit flies (Bactrocera papayae) infesting star fruit (Averrhoa carambola) based on mitochondrial 16S rRNA sequences
title_full	Phylogenetic tree construction in reconfirmation of parasitoid species (Braconidae: Opiinae), reared from fruit flies (Bactrocera papayae) infesting star fruit (Averrhoa carambola) based on mitochondrial 16S rRNA sequences
title_fullStr	Phylogenetic tree construction in reconfirmation of parasitoid species (Braconidae: Opiinae), reared from fruit flies (Bactrocera papayae) infesting star fruit (Averrhoa carambola) based on mitochondrial 16S rRNA sequences
title_full_unstemmed	Phylogenetic tree construction in reconfirmation of parasitoid species (Braconidae: Opiinae), reared from fruit flies (Bactrocera papayae) infesting star fruit (Averrhoa carambola) based on mitochondrial 16S rRNA sequences
title_sort	phylogenetic tree construction in reconfirmation of parasitoid species (braconidae: opiinae), reared from fruit flies (bactrocera papayae) infesting star fruit (averrhoa carambola) based on mitochondrial 16s rrna sequences
publishDate	2015
url	http://agris.upm.edu.my:8080/dspace/handle/0/9825
_version_	1782771072406388736
score	12.935284

Phylogenetic tree construction in reconfirmation of parasitoid species (Braconidae: Opiinae), reared from fruit flies (Bactrocera papayae) infesting star fruit (Averrhoa carambola) based on mitochondrial 16S rRNA sequences

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