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Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition

Microbial degradation of organic matters is crucial during the composting process. In this study, the enhancement of the composting of rice straw and chicken manure with biochar was evaluated by investigating the indigenous cellulolytic bacterial community structure during the composting process. Co...

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Main Authors: Mohd Zainudin, Mohd Huzairi, Singam, Jamuna Thurai, Sazili, Awis Qurni, Shirai, Yoshihito, Hassan, Mohd Ali
Format: Article
Published: Nature Publishing Group 2022
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spelling oai:psasir.upm.edu.my:101872 http://psasir.upm.edu.my/id/eprint/101872/ Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition Mohd Zainudin, Mohd Huzairi Singam, Jamuna Thurai Sazili, Awis Qurni Shirai, Yoshihito Hassan, Mohd Ali Microbial degradation of organic matters is crucial during the composting process. In this study, the enhancement of the composting of rice straw and chicken manure with biochar was evaluated by investigating the indigenous cellulolytic bacterial community structure during the composting process. Compared with control treatment, composting with biochar recorded higher temperature (74 °C), longer thermophilic phase (> 50 °C for 18 days) and reduced carbon (19%) with considerable micro- and macronutrients content. The bacterial community succession showed that composting with biochar was dominated by the cellulolytic Thermobifida and Nocardiopsis genera, which play an important role in lignocellulose degradation. Twenty-three cellulolytic bacterial strains were successfully isolated at different phases of the composting with biochar. The 16S rRNA gene sequencing similarity showed that they were related to Bacillus licheniformis, Bacillus subtilis, Bacillus aerius, and Bacillus haynesii, which were known as cellulolytic bacteria and generally involved in lignocellulose degradation. Of these isolated bacteria, Bacillus licheniformis, a facultative anaerobe, was the major bacterial strain isolated and demonstrated higher cellulase activities. The increase in temperature and reduction of carbon during the composting with biochar in this study can thus be attributed to the existence of these cellulolytic bacteria identified. Nature Publishing Group 2022-04-08 Article PeerReviewed Mohd Zainudin, Mohd Huzairi and Singam, Jamuna Thurai and Sazili, Awis Qurni and Shirai, Yoshihito and Hassan, Mohd Ali (2022) Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition. Scientific Reports, 12 (1). art. no. 5930. pp. 1-10. ISSN 2045-2322 https://www.nature.com/articles/s41598-022-09789-3 10.1038/s41598-022-09789-3
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description Microbial degradation of organic matters is crucial during the composting process. In this study, the enhancement of the composting of rice straw and chicken manure with biochar was evaluated by investigating the indigenous cellulolytic bacterial community structure during the composting process. Compared with control treatment, composting with biochar recorded higher temperature (74 °C), longer thermophilic phase (> 50 °C for 18 days) and reduced carbon (19%) with considerable micro- and macronutrients content. The bacterial community succession showed that composting with biochar was dominated by the cellulolytic Thermobifida and Nocardiopsis genera, which play an important role in lignocellulose degradation. Twenty-three cellulolytic bacterial strains were successfully isolated at different phases of the composting with biochar. The 16S rRNA gene sequencing similarity showed that they were related to Bacillus licheniformis, Bacillus subtilis, Bacillus aerius, and Bacillus haynesii, which were known as cellulolytic bacteria and generally involved in lignocellulose degradation. Of these isolated bacteria, Bacillus licheniformis, a facultative anaerobe, was the major bacterial strain isolated and demonstrated higher cellulase activities. The increase in temperature and reduction of carbon during the composting with biochar in this study can thus be attributed to the existence of these cellulolytic bacteria identified.
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author Mohd Zainudin, Mohd Huzairi
Singam, Jamuna Thurai
Sazili, Awis Qurni
Shirai, Yoshihito
Hassan, Mohd Ali
spellingShingle Mohd Zainudin, Mohd Huzairi
Singam, Jamuna Thurai
Sazili, Awis Qurni
Shirai, Yoshihito
Hassan, Mohd Ali
Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition
author_facet Mohd Zainudin, Mohd Huzairi
Singam, Jamuna Thurai
Sazili, Awis Qurni
Shirai, Yoshihito
Hassan, Mohd Ali
author_sort Mohd Zainudin, Mohd Huzairi
title Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition
title_short Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition
title_full Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition
title_fullStr Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition
title_full_unstemmed Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition
title_sort indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition
publisher Nature Publishing Group
publishDate 2022
_version_ 1819301303115841536
score 13.4562235

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