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Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia

Vibrio spp. are important aquaculture pathogens that cause vibriosis, affecting large numbers of marine fish species. This study determines the field efficacy of a feed-based inactivated vaccine against vibriosis in cage-cultured Asian seabass. A total of 4800 Asian seabass, kept in a field environm...

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Main Authors: Amir-Danial, Zahaludin, Saad, Mohd Zamri, Amal, Mohammad Noor Azmai, Annas, Salleh, Mohamad, Aslah, Jumria, Sutra, Manchanayake, Tilusha, Arbania, Ali, Ina-Salwany, Md Yasin
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Published: Multidisciplinary Digital Publishing Institute 2022
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spelling oai:psasir.upm.edu.my:108002 http://psasir.upm.edu.my/id/eprint/108002/ Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia Amir-Danial, Zahaludin Saad, Mohd Zamri Amal, Mohammad Noor Azmai Annas, Salleh Mohamad, Aslah Jumria, Sutra Manchanayake, Tilusha Arbania, Ali Ina-Salwany, Md Yasin Vibrio spp. are important aquaculture pathogens that cause vibriosis, affecting large numbers of marine fish species. This study determines the field efficacy of a feed-based inactivated vaccine against vibriosis in cage-cultured Asian seabass. A total of 4800 Asian seabass, kept in a field environment, were separated equally into two groups (vaccinated and non-vaccinated) in duplicate. Fish of Group 1 were orally administered the feed-based vaccine on weeks 0 (prime vaccination), 2 (booster), and 6 (second booster) at 4% body weight, while the non-vaccinated fish of Group 2 were fed with a commercial formulated pellet without the vaccine. Fish gut, mucus, and serum were collected, the length and weight of the fish were noted, while the mortality was recorded at 2-week intervals for a period of 16 weeks. The non-specific lysozyme activities were significantly (p < 0.05) higher in the fish of Group 1 than the non-vaccinated fish of Group 2. Similarly, the specific IgM antibody levels in serum and mucus were significantly (p < 0.05) higher in Group 1 than in Group 2, as seen in the second week, with the highest level 8 weeks after primary immunization. At week 16, the growth performance was significantly (p < 0.05) better in Group 1 and showed lower bacterial isolation in the gut than Group 2. Despite the statistical insignificance (p > 0.05), the survival rate was slightly higher in Group 1 (71.3%) than Group 2 (67.7%). This study revealed that feed-based vaccination improves growth performance, stimulates innate and adaptive immune responses, and increases protection of cultured Asian seabass, L. calcarifer, against vibriosis. Multidisciplinary Digital Publishing Institute 2022 Article PeerReviewed Amir-Danial, Zahaludin and Saad, Mohd Zamri and Amal, Mohammad Noor Azmai and Annas, Salleh and Mohamad, Aslah and Jumria, Sutra and Manchanayake, Tilusha and Arbania, Ali and Ina-Salwany, Md Yasin (2022) Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia. Vaccines, 11 (1). art. no. 9. pp. 1-13. ISSN 2076-393X https://www.mdpi.com/2076-393X/11/1/9 10.3390/vaccines11010009
institution UPM IR
collection UPM IR
description Vibrio spp. are important aquaculture pathogens that cause vibriosis, affecting large numbers of marine fish species. This study determines the field efficacy of a feed-based inactivated vaccine against vibriosis in cage-cultured Asian seabass. A total of 4800 Asian seabass, kept in a field environment, were separated equally into two groups (vaccinated and non-vaccinated) in duplicate. Fish of Group 1 were orally administered the feed-based vaccine on weeks 0 (prime vaccination), 2 (booster), and 6 (second booster) at 4% body weight, while the non-vaccinated fish of Group 2 were fed with a commercial formulated pellet without the vaccine. Fish gut, mucus, and serum were collected, the length and weight of the fish were noted, while the mortality was recorded at 2-week intervals for a period of 16 weeks. The non-specific lysozyme activities were significantly (p < 0.05) higher in the fish of Group 1 than the non-vaccinated fish of Group 2. Similarly, the specific IgM antibody levels in serum and mucus were significantly (p < 0.05) higher in Group 1 than in Group 2, as seen in the second week, with the highest level 8 weeks after primary immunization. At week 16, the growth performance was significantly (p < 0.05) better in Group 1 and showed lower bacterial isolation in the gut than Group 2. Despite the statistical insignificance (p > 0.05), the survival rate was slightly higher in Group 1 (71.3%) than Group 2 (67.7%). This study revealed that feed-based vaccination improves growth performance, stimulates innate and adaptive immune responses, and increases protection of cultured Asian seabass, L. calcarifer, against vibriosis.
format Article
author Amir-Danial, Zahaludin
Saad, Mohd Zamri
Amal, Mohammad Noor Azmai
Annas, Salleh
Mohamad, Aslah
Jumria, Sutra
Manchanayake, Tilusha
Arbania, Ali
Ina-Salwany, Md Yasin
spellingShingle Amir-Danial, Zahaludin
Saad, Mohd Zamri
Amal, Mohammad Noor Azmai
Annas, Salleh
Mohamad, Aslah
Jumria, Sutra
Manchanayake, Tilusha
Arbania, Ali
Ina-Salwany, Md Yasin
Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia
author_facet Amir-Danial, Zahaludin
Saad, Mohd Zamri
Amal, Mohammad Noor Azmai
Annas, Salleh
Mohamad, Aslah
Jumria, Sutra
Manchanayake, Tilusha
Arbania, Ali
Ina-Salwany, Md Yasin
author_sort Amir-Danial, Zahaludin
title Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia
title_short Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia
title_full Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia
title_fullStr Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia
title_full_unstemmed Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia
title_sort field efficacy of a feed-based inactivated vaccine against vibriosis in cage-cultured asian seabass, lates calcarifer, in malaysia
publisher Multidisciplinary Digital Publishing Institute
publishDate 2022
_version_ 1819301775665004544
score 13.4562235

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