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Probiotic strain Limosilactobacillus reuteri 29B is proven safe and exhibits potential probiotic traits in a murine vaginal model

Lactobacilli, the most common group of bacteria found in a healthy vaginal microbiota, have been demonstrated to act as a defence against colonisation and overgrowth of vaginal pathogens. These groups of bacteria have sparked interests in incorporating them as probiotics aimed at re-establishing bal...

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Main Authors: Rangasamy, Premmala, Foo, Hooi Ling, Mohd Yusof, Barakatun Nisak, Chew, Shu Yih, Mohd Jamil, Amilia Afzan, Than, Leslie Thian Lung
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Published: Springer 2023
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spelling oai:psasir.upm.edu.my:109136 http://psasir.upm.edu.my/id/eprint/109136/ Probiotic strain Limosilactobacillus reuteri 29B is proven safe and exhibits potential probiotic traits in a murine vaginal model Rangasamy, Premmala Foo, Hooi Ling Mohd Yusof, Barakatun Nisak Chew, Shu Yih Mohd Jamil, Amilia Afzan Than, Leslie Thian Lung Lactobacilli, the most common group of bacteria found in a healthy vaginal microbiota, have been demonstrated to act as a defence against colonisation and overgrowth of vaginal pathogens. These groups of bacteria have sparked interests in incorporating them as probiotics aimed at re-establishing balance within the urogenital ecosystem. In this study, the safety characteristics of Limosilactobacillus reuteri 29B (L29B) strain were evaluated through whole genome sequencing (WGS) and animal study. Cell culture assay and 16S rDNA analysis were done to evaluate the ability of the strain to colonise and adhere to the mouse vaginal tract, and RAST analysis was performed to screen for potential genes associated with probiotic trait. The histological study on the mice organs and blood analysis of the mice showed there was no incidence of inflammation. We also found no evidence of bacterial translocation. The cell culture assay on HeLa cells showed 85 of adhesion, and there was a significant reduction of Candida strain viability in displacement assay. As for the 16S rDNA analysis, there was a significant amount of L29B colonisation of the vaginal microflora. Taken together, the intravaginal administration of L29B significantly reduced the number Enterobacteriaceae and Staphylococcaceae that were present in mouse vaginal tract. It also improved and promoted a balanced vaginal microflora environment without causing any harm or irritation to mice. Limosilactobacillus 29B (L29B) is safe to be administered intravaginally. Springer 2023-06-14 Article PeerReviewed Rangasamy, Premmala and Foo, Hooi Ling and Mohd Yusof, Barakatun Nisak and Chew, Shu Yih and Mohd Jamil, Amilia Afzan and Than, Leslie Thian Lung (2023) Probiotic strain Limosilactobacillus reuteri 29B is proven safe and exhibits potential probiotic traits in a murine vaginal model. Probiotics and Antimicrobial Proteins, - (-). pp. 1-18. ISSN 1867-1306; ESSN: 1867-1314 https://link.springer.com/article/10.1007/s12602-023-10094-2 10.1007/s12602-023-10094-2
institution UPM IR
collection UPM IR
description Lactobacilli, the most common group of bacteria found in a healthy vaginal microbiota, have been demonstrated to act as a defence against colonisation and overgrowth of vaginal pathogens. These groups of bacteria have sparked interests in incorporating them as probiotics aimed at re-establishing balance within the urogenital ecosystem. In this study, the safety characteristics of Limosilactobacillus reuteri 29B (L29B) strain were evaluated through whole genome sequencing (WGS) and animal study. Cell culture assay and 16S rDNA analysis were done to evaluate the ability of the strain to colonise and adhere to the mouse vaginal tract, and RAST analysis was performed to screen for potential genes associated with probiotic trait. The histological study on the mice organs and blood analysis of the mice showed there was no incidence of inflammation. We also found no evidence of bacterial translocation. The cell culture assay on HeLa cells showed 85 of adhesion, and there was a significant reduction of Candida strain viability in displacement assay. As for the 16S rDNA analysis, there was a significant amount of L29B colonisation of the vaginal microflora. Taken together, the intravaginal administration of L29B significantly reduced the number Enterobacteriaceae and Staphylococcaceae that were present in mouse vaginal tract. It also improved and promoted a balanced vaginal microflora environment without causing any harm or irritation to mice. Limosilactobacillus 29B (L29B) is safe to be administered intravaginally.
format Article
author Rangasamy, Premmala
Foo, Hooi Ling
Mohd Yusof, Barakatun Nisak
Chew, Shu Yih
Mohd Jamil, Amilia Afzan
Than, Leslie Thian Lung
spellingShingle Rangasamy, Premmala
Foo, Hooi Ling
Mohd Yusof, Barakatun Nisak
Chew, Shu Yih
Mohd Jamil, Amilia Afzan
Than, Leslie Thian Lung
Probiotic strain Limosilactobacillus reuteri 29B is proven safe and exhibits potential probiotic traits in a murine vaginal model
author_facet Rangasamy, Premmala
Foo, Hooi Ling
Mohd Yusof, Barakatun Nisak
Chew, Shu Yih
Mohd Jamil, Amilia Afzan
Than, Leslie Thian Lung
author_sort Rangasamy, Premmala
title Probiotic strain Limosilactobacillus reuteri 29B is proven safe and exhibits potential probiotic traits in a murine vaginal model
title_short Probiotic strain Limosilactobacillus reuteri 29B is proven safe and exhibits potential probiotic traits in a murine vaginal model
title_full Probiotic strain Limosilactobacillus reuteri 29B is proven safe and exhibits potential probiotic traits in a murine vaginal model
title_fullStr Probiotic strain Limosilactobacillus reuteri 29B is proven safe and exhibits potential probiotic traits in a murine vaginal model
title_full_unstemmed Probiotic strain Limosilactobacillus reuteri 29B is proven safe and exhibits potential probiotic traits in a murine vaginal model
title_sort probiotic strain limosilactobacillus reuteri 29b is proven safe and exhibits potential probiotic traits in a murine vaginal model
publisher Springer
publishDate 2023
_version_ 1819301854915330048
score 13.4562235

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