Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix
Acetylcholinesterase (AChE) is a well-known enzyme sensitive to pesticide exposure, but heavy metal sensitivity is rarely reported. Here, AChE extracted from the brain tissue of Diodon hystrix was exploited and used in a fast and economical way to sensitively detect the existence of heavy metals in...
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Universiti Malaysia Terengganu
2023
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oai:psasir.upm.edu.my:109206 http://psasir.upm.edu.my/id/eprint/109206/ Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix Nordin, Noreen Abdulla, Rahmath Tahir, Rohana Jawan, Roslina Mohd Hamdan, Diana Demiyah Zahari, Nur Zaida Gansau, Jualang Azlan Shukor, Mohd Yunus Sabullah, Mohd Khalizan Acetylcholinesterase (AChE) is a well-known enzyme sensitive to pesticide exposure, but heavy metal sensitivity is rarely reported. Here, AChE extracted from the brain tissue of Diodon hystrix was exploited and used in a fast and economical way to sensitively detect the existence of heavy metals in a water sample. An inhibitive enzyme assay was conducted, and the activity of AChE was found to be sensitive (> 20% inhibition) to sub-million levels of arsenic, cadmium, nickel, and zinc and less sensitive (< 20% inhibition) to copper and lead. While exposure to argentum, cobalt, and chromium shows no significant inhibition, AChE is not sensitive to those metal ions (p < 0.05). Field test work has proved that the assay is suitable for preliminary detecting heavy metal contamination in the river, especially near industrial and mining sites. Secondary validation was performed using ICP-OES to identify and measure the number of elements in the sample and compare them to the inhibition level of AChE activity. Universiti Malaysia Terengganu 2023-02 Article PeerReviewed Nordin, Noreen and Abdulla, Rahmath and Tahir, Rohana and Jawan, Roslina and Mohd Hamdan, Diana Demiyah and Zahari, Nur Zaida and Gansau, Jualang Azlan and Shukor, Mohd Yunus and Sabullah, Mohd Khalizan (2023) Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix. Journal of Sustainability Science and Management, 18 (2). pp. 24-33. ISSN 1823-8556; ESSN: 2672-7226 https://jssm.umt.edu.my/wp-content/uploads/sites/51/2023/06/3-JSSM-Volume-18-Number-2-February-2023-FINAL.pdf 10.46754/jssm.2023.02.003 |
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UPM IR |
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UPM IR |
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Acetylcholinesterase (AChE) is a well-known enzyme sensitive to pesticide exposure, but heavy metal sensitivity is rarely reported. Here, AChE extracted from the brain tissue of Diodon hystrix was exploited and used in a fast and economical way to sensitively detect the existence of heavy metals in a water sample. An inhibitive enzyme assay was conducted, and the activity of AChE was found to be sensitive (> 20% inhibition) to sub-million levels of arsenic, cadmium, nickel, and zinc and less sensitive (< 20% inhibition) to copper and lead. While exposure to argentum, cobalt, and chromium shows no significant inhibition, AChE is not sensitive to those metal ions (p < 0.05). Field test work has proved that the assay is suitable for preliminary detecting heavy metal contamination in the river, especially near industrial and mining sites. Secondary validation was performed using ICP-OES to identify and measure the number of elements in the sample and compare them to the inhibition level of AChE activity. |
| format |
Article |
| author |
Nordin, Noreen Abdulla, Rahmath Tahir, Rohana Jawan, Roslina Mohd Hamdan, Diana Demiyah Zahari, Nur Zaida Gansau, Jualang Azlan Shukor, Mohd Yunus Sabullah, Mohd Khalizan |
| spellingShingle |
Nordin, Noreen Abdulla, Rahmath Tahir, Rohana Jawan, Roslina Mohd Hamdan, Diana Demiyah Zahari, Nur Zaida Gansau, Jualang Azlan Shukor, Mohd Yunus Sabullah, Mohd Khalizan Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix |
| author_facet |
Nordin, Noreen Abdulla, Rahmath Tahir, Rohana Jawan, Roslina Mohd Hamdan, Diana Demiyah Zahari, Nur Zaida Gansau, Jualang Azlan Shukor, Mohd Yunus Sabullah, Mohd Khalizan |
| author_sort |
Nordin, Noreen |
| title |
Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix |
| title_short |
Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix |
| title_full |
Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix |
| title_fullStr |
Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix |
| title_full_unstemmed |
Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix |
| title_sort |
preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from diodon hystrix |
| publisher |
Universiti Malaysia Terengganu |
| publishDate |
2023 |
| _version_ |
1819301861045305344 |
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13.4562235 |