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Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers

A series of experiments were conducted to isolate and characterize suitable intestinal microbes as direct-fed microbials and to investigate their effects on broiler. and the mechanisms associated with their utilization. The major types of bacteria found in the duodenum and jeju-ileum of chicken...

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Главный автор: Jin, Lizhi
Формат: Thesis
Язык:English
English
Опубликовано: 1996
Предметы:
Universiti Pertanian Malaysia - Dissertations
Bacterial diseases in poultry
Poultry - Infections
Online-ссылка:http://psasir.upm.edu.my/id/eprint/12319/1/FPV_1996_1_A.pdf
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id oai:psasir.upm.edu.my:12319
record_format eprints
spelling oai:psasir.upm.edu.my:12319 http://psasir.upm.edu.my/id/eprint/12319/ Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers Jin, Lizhi A series of experiments were conducted to isolate and characterize suitable intestinal microbes as direct-fed microbials and to investigate their effects on broiler. and the mechanisms associated with their utilization. The major types of bacteria found in the duodenum and jeju-ileum of chicken were Lactobacillus, Streptococcus and E. coli. In the cecum, ten groups of bacteria, namely, Streptococcus, Staphylococcus, Lactobacillus, E. coli, anaerobic coccus, Eubacterium, Propiombacterium, Clostridium, Fusobacterium and Bacteroides were isolated and identified. The main Lactobacillus species in chicken intestine were L. acidophilus, L. fermentum and L. brevis In vitro adhesion study demonstrated that 1 2 out of the 46 intestinal Lactobacillus isolates showed moderate to good ability to adhere to the ileal epithelial cell of chicken. Temperature (between 4°C and 42°C) did not have an effect on the attachment of the Lactobacillus isolates to the ileal epithelial cell. Incubation (contact) time of 30 min was found to be insufficient for the attachment of bacterial to the ileal epithelial cell, but contact time extended beyond 1 h did not improve the ability of the bacteria to adhere to the ileal epithelial cell. The pH values of suspending buffer for the Lactobacillus strains, ranging from 4 to 7, did not have any significant effect on the attachment of the bacteria to the ileal epithelial cell, but pH 8 reduced significantly (p<0.05) the attachment of bacteria. The growth of the 12 adherent Lactobacillus isolates was inhibited only slightly by the inclusion of 0.3% chicken bile in the culture medium, and they all showed a tolerance (in varying degrees) to low pH values ( 1.0 or 2.0). 1996-05 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12319/1/FPV_1996_1_A.pdf Jin, Lizhi (1996) Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers. PhD thesis, Universiti Pertanian Malaysia. Universiti Pertanian Malaysia - Dissertations Bacterial diseases in poultry Poultry - Infections English
institution UPM IR
collection UPM IR
language English
English
topic Universiti Pertanian Malaysia - Dissertations
Bacterial diseases in poultry
Poultry - Infections
spellingShingle Universiti Pertanian Malaysia - Dissertations
Bacterial diseases in poultry
Poultry - Infections
Jin, Lizhi
Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers
description A series of experiments were conducted to isolate and characterize suitable intestinal microbes as direct-fed microbials and to investigate their effects on broiler. and the mechanisms associated with their utilization. The major types of bacteria found in the duodenum and jeju-ileum of chicken were Lactobacillus, Streptococcus and E. coli. In the cecum, ten groups of bacteria, namely, Streptococcus, Staphylococcus, Lactobacillus, E. coli, anaerobic coccus, Eubacterium, Propiombacterium, Clostridium, Fusobacterium and Bacteroides were isolated and identified. The main Lactobacillus species in chicken intestine were L. acidophilus, L. fermentum and L. brevis In vitro adhesion study demonstrated that 1 2 out of the 46 intestinal Lactobacillus isolates showed moderate to good ability to adhere to the ileal epithelial cell of chicken. Temperature (between 4°C and 42°C) did not have an effect on the attachment of the Lactobacillus isolates to the ileal epithelial cell. Incubation (contact) time of 30 min was found to be insufficient for the attachment of bacterial to the ileal epithelial cell, but contact time extended beyond 1 h did not improve the ability of the bacteria to adhere to the ileal epithelial cell. The pH values of suspending buffer for the Lactobacillus strains, ranging from 4 to 7, did not have any significant effect on the attachment of the bacteria to the ileal epithelial cell, but pH 8 reduced significantly (p<0.05) the attachment of bacteria. The growth of the 12 adherent Lactobacillus isolates was inhibited only slightly by the inclusion of 0.3% chicken bile in the culture medium, and they all showed a tolerance (in varying degrees) to low pH values ( 1.0 or 2.0).
format Thesis
author Jin, Lizhi
author_facet Jin, Lizhi
author_sort Jin, Lizhi
title Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers
title_short Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers
title_full Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers
title_fullStr Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers
title_full_unstemmed Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers
title_sort studies on the mechanisms and utilization of probiotics (direct-fed microbials) in broilers
publishDate 1996
url http://psasir.upm.edu.my/id/eprint/12319/1/FPV_1996_1_A.pdf
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score 13.4562235

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