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Analysis of glutathione S-transferase (M1, T1 and P1) gene polymorphisms in Iranian prostate cancer subjects

Glutathione S-transferase enzymes are active in detoxifying a wide number of endogenous and exogenous chemical carcinogens and subsequently, are crucial in protecting the DNA. Several studies show some differences in association of glutathione S-transferase M1, T1 and P1 genetic polymorphisms with t...

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Main Authors: Basir, Sahar Ansari, Vasudevan, Ramachandran, Bakhshi, Ali, Mirinargesi, Mirsaed, Ismail, Patimah, Abdul Rahman, Sabariah, Pasalar, Parvin
Format: Article
Language:English
Published: Academic Journals 2010
Online Access:http://psasir.upm.edu.my/id/eprint/13079/1/13079.pdf
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spelling oai:psasir.upm.edu.my:13079 http://psasir.upm.edu.my/id/eprint/13079/ Analysis of glutathione S-transferase (M1, T1 and P1) gene polymorphisms in Iranian prostate cancer subjects Basir, Sahar Ansari Vasudevan, Ramachandran Bakhshi, Ali Mirinargesi, Mirsaed Ismail, Patimah Abdul Rahman, Sabariah Pasalar, Parvin Glutathione S-transferase enzymes are active in detoxifying a wide number of endogenous and exogenous chemical carcinogens and subsequently, are crucial in protecting the DNA. Several studies show some differences in association of glutathione S-transferase M1, T1 and P1 genetic polymorphisms with the risk of prostate cancer in various populations. The current study was done with Iranian subjects to evaluate the association of the polymorphism of glutathione S-transferase subtypes (T, M and P) and the susceptibility of prostate cancer in Iranian patients as compared to controls. Blood samples were collected from 65 prostate cancer patients and 65 unrelated health individuals as controls from Milad hospital, Tehran, Iran. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the polymorphism of glutathione S-transferase pi (GSTP1) -313 A/G gene, while multiplex PCR method was utilized to detect the glutathione S-transferase teta (GSTT) 1 and glutathione S-transferase mµ (GSTM) 1 null allele. There was no significant association in the -313 G allele (Val) of GSTP1 gene polymorphism and prostate cancer risk (odds ratio 0.61, 95% confidence intervals (CI) 0.08 -4.60, p = 0.627). Moreover, no relationship was found between the polymorphism of GSTT1 (odds ratio 0.66, 95% CI 0.27 -1.62) and GSTM1 (odds ratio 0.54, 95% CI 0.27 -1.08) genes and higher risk of prostate cancer among Iranian subjects (p > 0.05). This study showed that either GSTP1-313 G polymorphism or GSTT1 and GSTM1 genes cannot be predisposing risk factors for prostate cancer among Iranian subjects. Academic Journals 2010-10-25 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13079/1/13079.pdf Basir, Sahar Ansari and Vasudevan, Ramachandran and Bakhshi, Ali and Mirinargesi, Mirsaed and Ismail, Patimah and Abdul Rahman, Sabariah and Pasalar, Parvin (2010) Analysis of glutathione S-transferase (M1, T1 and P1) gene polymorphisms in Iranian prostate cancer subjects. African Journal of Biotechnology, 9 (43). art. no. 0B4631E25166. pp. 7230-7235. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/0B4631E25166 10.5897/AJB10.104
institution UPM IR
collection UPM IR
language English
description Glutathione S-transferase enzymes are active in detoxifying a wide number of endogenous and exogenous chemical carcinogens and subsequently, are crucial in protecting the DNA. Several studies show some differences in association of glutathione S-transferase M1, T1 and P1 genetic polymorphisms with the risk of prostate cancer in various populations. The current study was done with Iranian subjects to evaluate the association of the polymorphism of glutathione S-transferase subtypes (T, M and P) and the susceptibility of prostate cancer in Iranian patients as compared to controls. Blood samples were collected from 65 prostate cancer patients and 65 unrelated health individuals as controls from Milad hospital, Tehran, Iran. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the polymorphism of glutathione S-transferase pi (GSTP1) -313 A/G gene, while multiplex PCR method was utilized to detect the glutathione S-transferase teta (GSTT) 1 and glutathione S-transferase mµ (GSTM) 1 null allele. There was no significant association in the -313 G allele (Val) of GSTP1 gene polymorphism and prostate cancer risk (odds ratio 0.61, 95% confidence intervals (CI) 0.08 -4.60, p = 0.627). Moreover, no relationship was found between the polymorphism of GSTT1 (odds ratio 0.66, 95% CI 0.27 -1.62) and GSTM1 (odds ratio 0.54, 95% CI 0.27 -1.08) genes and higher risk of prostate cancer among Iranian subjects (p > 0.05). This study showed that either GSTP1-313 G polymorphism or GSTT1 and GSTM1 genes cannot be predisposing risk factors for prostate cancer among Iranian subjects.
format Article
author Basir, Sahar Ansari
Vasudevan, Ramachandran
Bakhshi, Ali
Mirinargesi, Mirsaed
Ismail, Patimah
Abdul Rahman, Sabariah
Pasalar, Parvin
spellingShingle Basir, Sahar Ansari
Vasudevan, Ramachandran
Bakhshi, Ali
Mirinargesi, Mirsaed
Ismail, Patimah
Abdul Rahman, Sabariah
Pasalar, Parvin
Analysis of glutathione S-transferase (M1, T1 and P1) gene polymorphisms in Iranian prostate cancer subjects
author_facet Basir, Sahar Ansari
Vasudevan, Ramachandran
Bakhshi, Ali
Mirinargesi, Mirsaed
Ismail, Patimah
Abdul Rahman, Sabariah
Pasalar, Parvin
author_sort Basir, Sahar Ansari
title Analysis of glutathione S-transferase (M1, T1 and P1) gene polymorphisms in Iranian prostate cancer subjects
title_short Analysis of glutathione S-transferase (M1, T1 and P1) gene polymorphisms in Iranian prostate cancer subjects
title_full Analysis of glutathione S-transferase (M1, T1 and P1) gene polymorphisms in Iranian prostate cancer subjects
title_fullStr Analysis of glutathione S-transferase (M1, T1 and P1) gene polymorphisms in Iranian prostate cancer subjects
title_full_unstemmed Analysis of glutathione S-transferase (M1, T1 and P1) gene polymorphisms in Iranian prostate cancer subjects
title_sort analysis of glutathione s-transferase (m1, t1 and p1) gene polymorphisms in iranian prostate cancer subjects
publisher Academic Journals
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/13079/1/13079.pdf
_version_ 1819293673167257600
score 13.4562235

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