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Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities

The influence of solvents with different polarities on the antioxidant and anti-inflammatory properties of betel leaf extracts (Piper betle) was investigated. The solvents used were water, ethanol, ethyl acetate and hexane. High performance liquid chromatography (HPLC) was used to determine the che...

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Main Authors: Pin, Kar Yong, Abdullah, Luqman Chuah, Ahmad, Abdull Rashih, Md. Pisar, Mazura, Jamaludin, Fadzureena, Subramaniam, Vimala, Mat Ali, Rasadah
Format: Article
Language:English
Published: Forest Research Institute Malaysia 2010
Online Access:http://psasir.upm.edu.my/id/eprint/13182/1/Antioxidant%20and%20anti.pdf
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spelling oai:psasir.upm.edu.my:13182 http://psasir.upm.edu.my/id/eprint/13182/ Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities Pin, Kar Yong Abdullah, Luqman Chuah Ahmad, Abdull Rashih Md. Pisar, Mazura Jamaludin, Fadzureena Subramaniam, Vimala Mat Ali, Rasadah The influence of solvents with different polarities on the antioxidant and anti-inflammatory properties of betel leaf extracts (Piper betle) was investigated. The solvents used were water, ethanol, ethyl acetate and hexane. High performance liquid chromatography (HPLC) was used to determine the chemical profiles and concentrations of the active compounds, namely, hydroxychavicol (HC) and eugenol (EU). The antioxidant potential of the extracts was evaluated using two in vitro assays—xanthine/xanthine oxidase superoxide scavenging assay (SOD assay) and 1,2-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay (DPPH assay). The anti-inflammatory assays used were hyaluronidase (HYA), xanthine oxidase (XOD) and lipoxygenase (LOX) inhibition assays. The HPLC results revealed that HC and EU were detected in all types of extracts and the concentrations were highest in the water extract. The highest extraction yield was obtained using water. All the extracts were highly active in both antioxidant assays with water extract showing the strongest inhibition. The extracts also exhibited significant inhibition in XOD and LOX assays. The results indicated that the bioactivity of the extracts was related to HC and EU. Forest Research Institute Malaysia 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13182/1/Antioxidant%20and%20anti.pdf Pin, Kar Yong and Abdullah, Luqman Chuah and Ahmad, Abdull Rashih and Md. Pisar, Mazura and Jamaludin, Fadzureena and Subramaniam, Vimala and Mat Ali, Rasadah (2010) Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities. Journal of Tropical Forest Science, 22 (4). pp. 448-455. ISSN 0128-1283 http://www.frim.gov.my/publication/journal-of-tropical-forest-science-jtfs/
institution UPM IR
collection UPM IR
language English
description The influence of solvents with different polarities on the antioxidant and anti-inflammatory properties of betel leaf extracts (Piper betle) was investigated. The solvents used were water, ethanol, ethyl acetate and hexane. High performance liquid chromatography (HPLC) was used to determine the chemical profiles and concentrations of the active compounds, namely, hydroxychavicol (HC) and eugenol (EU). The antioxidant potential of the extracts was evaluated using two in vitro assays—xanthine/xanthine oxidase superoxide scavenging assay (SOD assay) and 1,2-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay (DPPH assay). The anti-inflammatory assays used were hyaluronidase (HYA), xanthine oxidase (XOD) and lipoxygenase (LOX) inhibition assays. The HPLC results revealed that HC and EU were detected in all types of extracts and the concentrations were highest in the water extract. The highest extraction yield was obtained using water. All the extracts were highly active in both antioxidant assays with water extract showing the strongest inhibition. The extracts also exhibited significant inhibition in XOD and LOX assays. The results indicated that the bioactivity of the extracts was related to HC and EU.
format Article
author Pin, Kar Yong
Abdullah, Luqman Chuah
Ahmad, Abdull Rashih
Md. Pisar, Mazura
Jamaludin, Fadzureena
Subramaniam, Vimala
Mat Ali, Rasadah
spellingShingle Pin, Kar Yong
Abdullah, Luqman Chuah
Ahmad, Abdull Rashih
Md. Pisar, Mazura
Jamaludin, Fadzureena
Subramaniam, Vimala
Mat Ali, Rasadah
Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities
author_facet Pin, Kar Yong
Abdullah, Luqman Chuah
Ahmad, Abdull Rashih
Md. Pisar, Mazura
Jamaludin, Fadzureena
Subramaniam, Vimala
Mat Ali, Rasadah
author_sort Pin, Kar Yong
title Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities
title_short Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities
title_full Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities
title_fullStr Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities
title_full_unstemmed Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities
title_sort antioxidant and anti-inflammatory activities of extracts of betel leaves (piper betle) from solvents with different polarities
publisher Forest Research Institute Malaysia
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/13182/1/Antioxidant%20and%20anti.pdf
_version_ 1819293684538015744
score 13.4562235

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