Application of horseshoe crab-turbidity basis in the development of a sensitive detection assay for gram negative.
Quantitation assay of Escherichia coli, Samonell sp. and Vibrio cholerae cells investigated by exploiting the component consistently present on the outer surface of Gram-negative bacteria. In this study, a simple marine biolysate-based method for simultaneous detection of the gram negative pathogeni...
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| Язык: | English |
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Medwell Journals
2008
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| Online-ссылка: | http://psasir.upm.edu.my/id/eprint/13230/1/Application%20of%20horseshoe%20crab.pdf |
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oai:psasir.upm.edu.my:13230 http://psasir.upm.edu.my/id/eprint/13230/ Application of horseshoe crab-turbidity basis in the development of a sensitive detection assay for gram negative. Al-Haj, Nagi A. Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri Quantitation assay of Escherichia coli, Samonell sp. and Vibrio cholerae cells investigated by exploiting the component consistently present on the outer surface of Gram-negative bacteria. In this study, a simple marine biolysate-based method for simultaneous detection of the gram negative pathogenic bacteria based on lipopolysaccharide component was optimized. The detection technique focused on the surface of these bacterial species, which is covered by polysaccharides and has high affinity to marine biolysate. E. coli, Samonell sp. and V. cholerae with similar initial cell count per mL have different but consistent absorbance readings by using the spectrophotometer and turbidity meter. This revealed that different genera of Gram Negative bacteria can be directly differentiated through standard curve that is plotted from the carbohydrate and marine biolysate assays absorbance readings. Both the assays elucidated a qualitative and quantitative detection of the pure culture pathogens. Medwell Journals 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13230/1/Application%20of%20horseshoe%20crab.pdf Al-Haj, Nagi A. and Shamsudin, Mariana Nor and Abdul Rahim, Raha and Ishak, Zamri (2008) Application of horseshoe crab-turbidity basis in the development of a sensitive detection assay for gram negative. Research Journal of Biological Sciences, 3 (12). pp. 1387-1391. ISSN 1815-8846; ESSN: 1993-6087 http://medwelljournals.com/abstract/?doi=rjbsci.2008.1387.1391 |
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| language |
English |
| description |
Quantitation assay of Escherichia coli, Samonell sp. and Vibrio cholerae cells investigated by exploiting the component consistently present on the outer surface of Gram-negative bacteria. In this study, a simple marine biolysate-based method for simultaneous detection of the gram negative pathogenic bacteria based on lipopolysaccharide component was optimized. The detection technique focused on the surface of these bacterial species, which is covered by polysaccharides and has high affinity to marine biolysate. E. coli, Samonell sp. and V. cholerae with similar initial cell count per mL have different but consistent absorbance readings by using the spectrophotometer and turbidity meter. This revealed that different genera of Gram Negative bacteria can be directly differentiated through standard curve that is plotted from the carbohydrate and marine biolysate assays absorbance readings. Both the assays elucidated a qualitative and quantitative detection of the pure culture pathogens. |
| format |
Article |
| author |
Al-Haj, Nagi A. Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri |
| spellingShingle |
Al-Haj, Nagi A. Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri Application of horseshoe crab-turbidity basis in the development of a sensitive detection assay for gram negative. |
| author_facet |
Al-Haj, Nagi A. Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri |
| author_sort |
Al-Haj, Nagi A. |
| title |
Application of horseshoe crab-turbidity basis in the development of a sensitive detection assay for gram negative. |
| title_short |
Application of horseshoe crab-turbidity basis in the development of a sensitive detection assay for gram negative. |
| title_full |
Application of horseshoe crab-turbidity basis in the development of a sensitive detection assay for gram negative. |
| title_fullStr |
Application of horseshoe crab-turbidity basis in the development of a sensitive detection assay for gram negative. |
| title_full_unstemmed |
Application of horseshoe crab-turbidity basis in the development of a sensitive detection assay for gram negative. |
| title_sort |
application of horseshoe crab-turbidity basis in the development of a sensitive detection assay for gram negative. |
| publisher |
Medwell Journals |
| publishDate |
2008 |
| url |
http://psasir.upm.edu.my/id/eprint/13230/1/Application%20of%20horseshoe%20crab.pdf |
| _version_ |
1819293689603686400 |
| score |
13.4562235 |