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Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model

The objective of this study was to regenerate the tracheal epithelium using autologous nasal respiratory epithelial cells in a sheep model. Respiratory epithelium and fibroblast cells were harvested from nasal turbinates and cultured for 1 week. After confluence, respiratory epithelium and fibroblas...

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Hoofdauteurs: Mohd Yunus, Mohd Heikal, Saim, Aminuddin, Jahendran, Jeevanan, Chen, Hui Cheng, Syed Hussain, Sharifah Salmah, Idrus, Ruszymah
Formaat: Artikel
Taal:English
Gepubliceerd in: S. Karger 2010
Online toegang:http://psasir.upm.edu.my/id/eprint/13337/1/Autologous%20implantation%20of%20bilayered%20tissue.pdf
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spelling oai:psasir.upm.edu.my:13337 http://psasir.upm.edu.my/id/eprint/13337/ Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model Mohd Yunus, Mohd Heikal Saim, Aminuddin Jahendran, Jeevanan Chen, Hui Cheng Syed Hussain, Sharifah Salmah Idrus, Ruszymah The objective of this study was to regenerate the tracheal epithelium using autologous nasal respiratory epithelial cells in a sheep model. Respiratory epithelium and fibroblast cells were harvested from nasal turbinates and cultured for 1 week. After confluence, respiratory epithelium and fibroblast cells were suspended in autologous fibrin polymerized separately to form a tissue-engineered respiratory epithelial construct (TEREC). A 3 × 2 cm2 tracheal mucosal defect was created, and implanted with TEREC and titanium mesh as a temporary scaffold. The control groups were divided into 2 groups: polymerized autologous fibrin devoid of cells (group 1), and no construct implanted (group 2). All sheep were euthanized at 4 weeks of implantation. Gross observation of the trachea showed minimal luminal stenosis formation in the experimental group compared to the control groups. Macroscopic evaluation revealed significant mucosal fibrosis in control group 1 (71.8%) as compared to the experimental group (7%). Hematoxylin and eosin staining revealed the presence of minimal overgrowth of fibrous connective tissue covered by respiratory epithelium. A positive red fluorescence staining of PKH26 on engineered tissue 4 weeks after implantation confirmed the presence of cultured nasal respiratory epithelial cells intercalated with native tracheal epithelial cells. Scanning electron microscopy showed the presence of short microvilli representing immature cilia on the surface of the epithelium. Our study showed that TEREC was a good replacement for a tracheal mucosal defect and was able to promote natural regenesis of the tracheal epithelium with minimal fibrosis. This study highlighted a new technique in the treatment of tracheal stenosis. S. Karger 2010-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13337/1/Autologous%20implantation%20of%20bilayered%20tissue.pdf Mohd Yunus, Mohd Heikal and Saim, Aminuddin and Jahendran, Jeevanan and Chen, Hui Cheng and Syed Hussain, Sharifah Salmah and Idrus, Ruszymah (2010) Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model. Cells Tissues Organs, 192 (5). pp. 292-302. ISSN 1422-6405; ESSN: 1422-6421 10.1159/000318675
institution UPM IR
collection UPM IR
language English
description The objective of this study was to regenerate the tracheal epithelium using autologous nasal respiratory epithelial cells in a sheep model. Respiratory epithelium and fibroblast cells were harvested from nasal turbinates and cultured for 1 week. After confluence, respiratory epithelium and fibroblast cells were suspended in autologous fibrin polymerized separately to form a tissue-engineered respiratory epithelial construct (TEREC). A 3 × 2 cm2 tracheal mucosal defect was created, and implanted with TEREC and titanium mesh as a temporary scaffold. The control groups were divided into 2 groups: polymerized autologous fibrin devoid of cells (group 1), and no construct implanted (group 2). All sheep were euthanized at 4 weeks of implantation. Gross observation of the trachea showed minimal luminal stenosis formation in the experimental group compared to the control groups. Macroscopic evaluation revealed significant mucosal fibrosis in control group 1 (71.8%) as compared to the experimental group (7%). Hematoxylin and eosin staining revealed the presence of minimal overgrowth of fibrous connective tissue covered by respiratory epithelium. A positive red fluorescence staining of PKH26 on engineered tissue 4 weeks after implantation confirmed the presence of cultured nasal respiratory epithelial cells intercalated with native tracheal epithelial cells. Scanning electron microscopy showed the presence of short microvilli representing immature cilia on the surface of the epithelium. Our study showed that TEREC was a good replacement for a tracheal mucosal defect and was able to promote natural regenesis of the tracheal epithelium with minimal fibrosis. This study highlighted a new technique in the treatment of tracheal stenosis.
format Article
author Mohd Yunus, Mohd Heikal
Saim, Aminuddin
Jahendran, Jeevanan
Chen, Hui Cheng
Syed Hussain, Sharifah Salmah
Idrus, Ruszymah
spellingShingle Mohd Yunus, Mohd Heikal
Saim, Aminuddin
Jahendran, Jeevanan
Chen, Hui Cheng
Syed Hussain, Sharifah Salmah
Idrus, Ruszymah
Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model
author_facet Mohd Yunus, Mohd Heikal
Saim, Aminuddin
Jahendran, Jeevanan
Chen, Hui Cheng
Syed Hussain, Sharifah Salmah
Idrus, Ruszymah
author_sort Mohd Yunus, Mohd Heikal
title Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model
title_short Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model
title_full Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model
title_fullStr Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model
title_full_unstemmed Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model
title_sort autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model
publisher S. Karger
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/13337/1/Autologous%20implantation%20of%20bilayered%20tissue.pdf
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score 13.4562235

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