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Bioconversion of oil empty fruit bunch by Aspergillus niger EB4 under solid-state cultivation

Oil palm empty fruit bunch (OPEFB) is an abundant lignocellulosic waste material generated from the palm oil industry. In this study, a locally isolated strain Aspergillus niger EB4 was cultivated on a pre-treated OPEFB as substrate to produce cellulase in the solid-state fermentation (SSF) process....

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Hlavní autoři: Samsu Baharuddin, Azhari, Abd Razak, Nor Asma, Abdul Rahman, Nor'aini, Budiatman, Satiawihardja, Shirai, Yoshihito, Hassan, Mohd Ali
Médium: Článek
Jazyk:English
Vydáno: Universiti Putra Malaysia Press 2009
On-line přístup:http://psasir.upm.edu.my/id/eprint/13404/1/09%20Pages%20143-151.pdf
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spelling oai:psasir.upm.edu.my:13404 http://psasir.upm.edu.my/id/eprint/13404/ Bioconversion of oil empty fruit bunch by Aspergillus niger EB4 under solid-state cultivation Samsu Baharuddin, Azhari Abd Razak, Nor Asma Abdul Rahman, Nor'aini Budiatman, Satiawihardja Shirai, Yoshihito Hassan, Mohd Ali Oil palm empty fruit bunch (OPEFB) is an abundant lignocellulosic waste material generated from the palm oil industry. In this study, a locally isolated strain Aspergillus niger EB4 was cultivated on a pre-treated OPEFB as substrate to produce cellulase in the solid-state fermentation (SSF) process. The cellulase recovered was then subjected to a saccharification process. The strain was grown on the pre-treated OPEFB in a 250 ml Erlenmeyer flask and a 192 L tray cabinet bioreactor, at 80% moisture content and incubated for nine days under a static condition. The activities of the crude cellulase extract in the tray bioreactor were 19.02 ± 0.85, 6.36 ± 0.38 and 4.56 ± 0.26 U/g for β-glucosidase, CMCase and FPase, respectively on day 6 of fermentation. These results were similar to the results obtained from the flask experiment. The results demonstrated the feasibility of solid substrate fermentation of the OPEFB in both flask and tray cabinet bioreactor for the cellulase production. The enzymatic hydrolysis of OPEFB at 5% (w/v) was performed by utilizing the partially purified and crude cellulase incubated at 40°C for seven days. The partially purified cellullases hydrolyzed the pre-treated OPEFB and released 7.7 g/l of reducing sugar which corresponded to a 15% conversion. Universiti Putra Malaysia Press 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13404/1/09%20Pages%20143-151.pdf Samsu Baharuddin, Azhari and Abd Razak, Nor Asma and Abdul Rahman, Nor'aini and Budiatman, Satiawihardja and Shirai, Yoshihito and Hassan, Mohd Ali (2009) Bioconversion of oil empty fruit bunch by Aspergillus niger EB4 under solid-state cultivation. Pertanika Journal of Tropical Agricultural Science, 32 (2). pp. 143-151. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2032%20(2)%20Aug.%202009/09%20Pages%20143-151.pdf
institution UPM IR
collection UPM IR
language English
description Oil palm empty fruit bunch (OPEFB) is an abundant lignocellulosic waste material generated from the palm oil industry. In this study, a locally isolated strain Aspergillus niger EB4 was cultivated on a pre-treated OPEFB as substrate to produce cellulase in the solid-state fermentation (SSF) process. The cellulase recovered was then subjected to a saccharification process. The strain was grown on the pre-treated OPEFB in a 250 ml Erlenmeyer flask and a 192 L tray cabinet bioreactor, at 80% moisture content and incubated for nine days under a static condition. The activities of the crude cellulase extract in the tray bioreactor were 19.02 ± 0.85, 6.36 ± 0.38 and 4.56 ± 0.26 U/g for β-glucosidase, CMCase and FPase, respectively on day 6 of fermentation. These results were similar to the results obtained from the flask experiment. The results demonstrated the feasibility of solid substrate fermentation of the OPEFB in both flask and tray cabinet bioreactor for the cellulase production. The enzymatic hydrolysis of OPEFB at 5% (w/v) was performed by utilizing the partially purified and crude cellulase incubated at 40°C for seven days. The partially purified cellullases hydrolyzed the pre-treated OPEFB and released 7.7 g/l of reducing sugar which corresponded to a 15% conversion.
format Article
author Samsu Baharuddin, Azhari
Abd Razak, Nor Asma
Abdul Rahman, Nor'aini
Budiatman, Satiawihardja
Shirai, Yoshihito
Hassan, Mohd Ali
spellingShingle Samsu Baharuddin, Azhari
Abd Razak, Nor Asma
Abdul Rahman, Nor'aini
Budiatman, Satiawihardja
Shirai, Yoshihito
Hassan, Mohd Ali
Bioconversion of oil empty fruit bunch by Aspergillus niger EB4 under solid-state cultivation
author_facet Samsu Baharuddin, Azhari
Abd Razak, Nor Asma
Abdul Rahman, Nor'aini
Budiatman, Satiawihardja
Shirai, Yoshihito
Hassan, Mohd Ali
author_sort Samsu Baharuddin, Azhari
title Bioconversion of oil empty fruit bunch by Aspergillus niger EB4 under solid-state cultivation
title_short Bioconversion of oil empty fruit bunch by Aspergillus niger EB4 under solid-state cultivation
title_full Bioconversion of oil empty fruit bunch by Aspergillus niger EB4 under solid-state cultivation
title_fullStr Bioconversion of oil empty fruit bunch by Aspergillus niger EB4 under solid-state cultivation
title_full_unstemmed Bioconversion of oil empty fruit bunch by Aspergillus niger EB4 under solid-state cultivation
title_sort bioconversion of oil empty fruit bunch by aspergillus niger eb4 under solid-state cultivation
publisher Universiti Putra Malaysia Press
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/13404/1/09%20Pages%20143-151.pdf
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score 13.4562235

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