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Detection of diarrheagenic Escherichia coli isolated using molecular approaches.

Escherichia coli strains are among the major bacterial causes of diarrheal illness. There are now seven classes of diarrheagenic E. coli (DEC), namely enteropathogenic E. coli (EPEC), enterohaemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), enteroaggregative...

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Main Authors: Abdullah Al-Haj, Nagi Ahmed, Shamsudin, Mariana Nor, Abdul Rahim, Raha, Ishak, Zamri
Format: Article
Language:English
English
Published: Academic Journals 2008
Online Access:http://psasir.upm.edu.my/id/eprint/13931/1/Detection%20of%20diarrheagenic%20Escherichia%20coli%20isolated%20using%20molecular%20approaches.pdf
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spelling oai:psasir.upm.edu.my:13931 http://psasir.upm.edu.my/id/eprint/13931/ Detection of diarrheagenic Escherichia coli isolated using molecular approaches. Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri Escherichia coli strains are among the major bacterial causes of diarrheal illness. There are now seven classes of diarrheagenic E. coli (DEC), namely enteropathogenic E. coli (EPEC), enterohaemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), enteroaggregative E. coli (EAEC), diarrhea-associated hemolytic E. coli (DHEC) and Cytolethal Distending Toxin (CDT)-producing E. coli. Due to the need for costly and labor-intensive diagnostic procedures, identification of DEC is difficult at standard laboratories. Therefore, Polymerase Chain Reaction (PCR) or dot blot has been used for genetic detection of DEC of 25 E. coli isolates from different sources. Amplification of eae (277 bp), bfp (266 bp), stx1 (154 bp), EAST (94 bp), stx2 (698 bp) and elt (450 bp) genes of a single product in separate reactions was produced. PCR showed ability to amplify and detected genes of the most common important categories of diarrheagenic E. coli isolates of different sources, it is possible implementation of this technique to diagnosis water, foodborne outbreaks related to E. coli. Dots blot and sequence analysis used to confirm the results of PCR. Academic Journals 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13931/1/Detection%20of%20diarrheagenic%20Escherichia%20coli%20isolated%20using%20molecular%20approaches.pdf Abdullah Al-Haj, Nagi Ahmed and Shamsudin, Mariana Nor and Abdul Rahim, Raha and Ishak, Zamri (2008) Detection of diarrheagenic Escherichia coli isolated using molecular approaches. Research Journal of Microbiology, 3 (5). pp. 359-367. ISSN 1816-4935 10.3923/jm.2008.359.367 English
institution UPM IR
collection UPM IR
language English
English
description Escherichia coli strains are among the major bacterial causes of diarrheal illness. There are now seven classes of diarrheagenic E. coli (DEC), namely enteropathogenic E. coli (EPEC), enterohaemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), enteroaggregative E. coli (EAEC), diarrhea-associated hemolytic E. coli (DHEC) and Cytolethal Distending Toxin (CDT)-producing E. coli. Due to the need for costly and labor-intensive diagnostic procedures, identification of DEC is difficult at standard laboratories. Therefore, Polymerase Chain Reaction (PCR) or dot blot has been used for genetic detection of DEC of 25 E. coli isolates from different sources. Amplification of eae (277 bp), bfp (266 bp), stx1 (154 bp), EAST (94 bp), stx2 (698 bp) and elt (450 bp) genes of a single product in separate reactions was produced. PCR showed ability to amplify and detected genes of the most common important categories of diarrheagenic E. coli isolates of different sources, it is possible implementation of this technique to diagnosis water, foodborne outbreaks related to E. coli. Dots blot and sequence analysis used to confirm the results of PCR.
format Article
author Abdullah Al-Haj, Nagi Ahmed
Shamsudin, Mariana Nor
Abdul Rahim, Raha
Ishak, Zamri
spellingShingle Abdullah Al-Haj, Nagi Ahmed
Shamsudin, Mariana Nor
Abdul Rahim, Raha
Ishak, Zamri
Detection of diarrheagenic Escherichia coli isolated using molecular approaches.
author_facet Abdullah Al-Haj, Nagi Ahmed
Shamsudin, Mariana Nor
Abdul Rahim, Raha
Ishak, Zamri
author_sort Abdullah Al-Haj, Nagi Ahmed
title Detection of diarrheagenic Escherichia coli isolated using molecular approaches.
title_short Detection of diarrheagenic Escherichia coli isolated using molecular approaches.
title_full Detection of diarrheagenic Escherichia coli isolated using molecular approaches.
title_fullStr Detection of diarrheagenic Escherichia coli isolated using molecular approaches.
title_full_unstemmed Detection of diarrheagenic Escherichia coli isolated using molecular approaches.
title_sort detection of diarrheagenic escherichia coli isolated using molecular approaches.
publisher Academic Journals
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/13931/1/Detection%20of%20diarrheagenic%20Escherichia%20coli%20isolated%20using%20molecular%20approaches.pdf
_version_ 1819293761988984832
score 13.4562235

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