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Detection of pathogenic Leptospira from selected environment in Kelantan and Terengganu, Malaysia

Leptospirosis is recognized as one of the important zoonotic diseases in the world including Malaysia. A total of 145 soil and water samples were collected from selected National Service Training Centres (NSTC) in Kelantan and Terengganu. The samples were inoculated into modified semisolid Ellinghau...

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Main Authors: A. Rashid, Fairuz Ridzlan, Bahaman, Abdul Rani, Bejo, Siti Khairani, Mutalib, Abdul Rahim
Format: Article
Language:English
Published: Malaysian Society of Parasitology and Tropical Medicine 2010
Online Access:http://psasir.upm.edu.my/id/eprint/13936/1/Detection%20of%20pathogenic%20Leptospira%20from%20selected%20environment%20in%20Kelantan%20and%20Terengganu.pdf
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spelling oai:psasir.upm.edu.my:13936 http://psasir.upm.edu.my/id/eprint/13936/ Detection of pathogenic Leptospira from selected environment in Kelantan and Terengganu, Malaysia A. Rashid, Fairuz Ridzlan Bahaman, Abdul Rani Bejo, Siti Khairani Mutalib, Abdul Rahim Leptospirosis is recognized as one of the important zoonotic diseases in the world including Malaysia. A total of 145 soil and water samples were collected from selected National Service Training Centres (NSTC) in Kelantan and Terengganu. The samples were inoculated into modified semisolid Ellinghausen McCullough Johnson Harris (EMJH) medium, incubated at room temperature for 1 month and examined under the dark-field microscope. Positive growth of the leptospiral isolates were then confirmed with 8-Azaguanine Test, Polymerase Chain Reaction (PCR) assay and Microscopic Agglutination Test (MAT). Fifteen cultures (10.34%) exhibited positive growths which were seen under dark field microscope whilst only 20% (3/15) were confirmed as pathogenic species. based on 8-Azaguanine Test and PCR. Serological identification of the isolates with MAT showed that hebdomadis was the dominant serovar in Terengganu. Pathogenic leptospires can be detected in Malaysian environment and this has the potential to cause an outbreak. Therefore, precautionary steps against leptospirosis should be taken by camp authorities to ensure the safety of trainees. Malaysian Society of Parasitology and Tropical Medicine 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13936/1/Detection%20of%20pathogenic%20Leptospira%20from%20selected%20environment%20in%20Kelantan%20and%20Terengganu.pdf A. Rashid, Fairuz Ridzlan and Bahaman, Abdul Rani and Bejo, Siti Khairani and Mutalib, Abdul Rahim (2010) Detection of pathogenic Leptospira from selected environment in Kelantan and Terengganu, Malaysia. Tropical Biomedicine, 27 (3). pp. 632-638. ISSN 0127-5720 http://www.msptm.org/journal17.html
institution UPM IR
collection UPM IR
language English
description Leptospirosis is recognized as one of the important zoonotic diseases in the world including Malaysia. A total of 145 soil and water samples were collected from selected National Service Training Centres (NSTC) in Kelantan and Terengganu. The samples were inoculated into modified semisolid Ellinghausen McCullough Johnson Harris (EMJH) medium, incubated at room temperature for 1 month and examined under the dark-field microscope. Positive growth of the leptospiral isolates were then confirmed with 8-Azaguanine Test, Polymerase Chain Reaction (PCR) assay and Microscopic Agglutination Test (MAT). Fifteen cultures (10.34%) exhibited positive growths which were seen under dark field microscope whilst only 20% (3/15) were confirmed as pathogenic species. based on 8-Azaguanine Test and PCR. Serological identification of the isolates with MAT showed that hebdomadis was the dominant serovar in Terengganu. Pathogenic leptospires can be detected in Malaysian environment and this has the potential to cause an outbreak. Therefore, precautionary steps against leptospirosis should be taken by camp authorities to ensure the safety of trainees.
format Article
author A. Rashid, Fairuz Ridzlan
Bahaman, Abdul Rani
Bejo, Siti Khairani
Mutalib, Abdul Rahim
spellingShingle A. Rashid, Fairuz Ridzlan
Bahaman, Abdul Rani
Bejo, Siti Khairani
Mutalib, Abdul Rahim
Detection of pathogenic Leptospira from selected environment in Kelantan and Terengganu, Malaysia
author_facet A. Rashid, Fairuz Ridzlan
Bahaman, Abdul Rani
Bejo, Siti Khairani
Mutalib, Abdul Rahim
author_sort A. Rashid, Fairuz Ridzlan
title Detection of pathogenic Leptospira from selected environment in Kelantan and Terengganu, Malaysia
title_short Detection of pathogenic Leptospira from selected environment in Kelantan and Terengganu, Malaysia
title_full Detection of pathogenic Leptospira from selected environment in Kelantan and Terengganu, Malaysia
title_fullStr Detection of pathogenic Leptospira from selected environment in Kelantan and Terengganu, Malaysia
title_full_unstemmed Detection of pathogenic Leptospira from selected environment in Kelantan and Terengganu, Malaysia
title_sort detection of pathogenic leptospira from selected environment in kelantan and terengganu, malaysia
publisher Malaysian Society of Parasitology and Tropical Medicine
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/13936/1/Detection%20of%20pathogenic%20Leptospira%20from%20selected%20environment%20in%20Kelantan%20and%20Terengganu.pdf
_version_ 1819293762461892608
score 13.4562235

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