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Polymerase chain reaction and cloning of Burkholderia pseudomallei putative genes.

Total of 23 putative Open Read Farms from B. pseudomallei strain D286 was successfully cloned and the nucleotide sequence analysis of the putative genes showed the homologue (98-100%) to strain K96243. The high similarity in gene sequences between these strains is confirmed for presence of the neces...

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Autors principals: Abdullah Al-Haj, Nagi Ahmed, Lai, L. Suang, Shamsudin, Mariana Nor, Abdullah, Rasedee, Mohamed, Rahmah, Sekawi, Zamberi
Format: Article
Idioma:English
English
Publicat: Medwell Publishing 2009
Accés en línia:http://psasir.upm.edu.my/id/eprint/16240/1/Polymerase%20chain%20reaction%20and%20cloning%20of%20Burkholderia%20pseudomallei%20putative%20genes.pdf
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spelling oai:psasir.upm.edu.my:16240 http://psasir.upm.edu.my/id/eprint/16240/ Polymerase chain reaction and cloning of Burkholderia pseudomallei putative genes. Abdullah Al-Haj, Nagi Ahmed Lai, L. Suang Shamsudin, Mariana Nor Abdullah, Rasedee Mohamed, Rahmah Sekawi, Zamberi Total of 23 putative Open Read Farms from B. pseudomallei strain D286 was successfully cloned and the nucleotide sequence analysis of the putative genes showed the homologue (98-100%) to strain K96243. The high similarity in gene sequences between these strains is confirmed for presence of the necessary ORF for LPS biosynthesis through PCR amplification the application of the ORFs in the PCR amplification and expression method. The findings of this study have contributed to some information on the molecular bases of the LPS biosynthesis genes in B. pseudomallei specifically for strain D286. PCR amplification, a specific pair of primer for each ORFs was proving specific for amplification of genes in B. pseudomallei strain D286. The PCR mixture with addition of DMSO, formamide and glycerol could ease the PCR optimization where different pairs of primers were involved. The specific primer pairs with the PCR mixture could be used in developing a PCR diagnosis of melioidosis. Medwell Publishing 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16240/1/Polymerase%20chain%20reaction%20and%20cloning%20of%20Burkholderia%20pseudomallei%20putative%20genes.pdf Abdullah Al-Haj, Nagi Ahmed and Lai, L. Suang and Shamsudin, Mariana Nor and Abdullah, Rasedee and Mohamed, Rahmah and Sekawi, Zamberi (2009) Polymerase chain reaction and cloning of Burkholderia pseudomallei putative genes. Research Journal of Medical Sciences, 3 (3). pp. 104-110. ISSN 1815-9346; ESSN : 1993-6095 rjmsci.2009.104.110 English
institution UPM IR
collection UPM IR
language English
English
description Total of 23 putative Open Read Farms from B. pseudomallei strain D286 was successfully cloned and the nucleotide sequence analysis of the putative genes showed the homologue (98-100%) to strain K96243. The high similarity in gene sequences between these strains is confirmed for presence of the necessary ORF for LPS biosynthesis through PCR amplification the application of the ORFs in the PCR amplification and expression method. The findings of this study have contributed to some information on the molecular bases of the LPS biosynthesis genes in B. pseudomallei specifically for strain D286. PCR amplification, a specific pair of primer for each ORFs was proving specific for amplification of genes in B. pseudomallei strain D286. The PCR mixture with addition of DMSO, formamide and glycerol could ease the PCR optimization where different pairs of primers were involved. The specific primer pairs with the PCR mixture could be used in developing a PCR diagnosis of melioidosis.
format Article
author Abdullah Al-Haj, Nagi Ahmed
Lai, L. Suang
Shamsudin, Mariana Nor
Abdullah, Rasedee
Mohamed, Rahmah
Sekawi, Zamberi
spellingShingle Abdullah Al-Haj, Nagi Ahmed
Lai, L. Suang
Shamsudin, Mariana Nor
Abdullah, Rasedee
Mohamed, Rahmah
Sekawi, Zamberi
Polymerase chain reaction and cloning of Burkholderia pseudomallei putative genes.
author_facet Abdullah Al-Haj, Nagi Ahmed
Lai, L. Suang
Shamsudin, Mariana Nor
Abdullah, Rasedee
Mohamed, Rahmah
Sekawi, Zamberi
author_sort Abdullah Al-Haj, Nagi Ahmed
title Polymerase chain reaction and cloning of Burkholderia pseudomallei putative genes.
title_short Polymerase chain reaction and cloning of Burkholderia pseudomallei putative genes.
title_full Polymerase chain reaction and cloning of Burkholderia pseudomallei putative genes.
title_fullStr Polymerase chain reaction and cloning of Burkholderia pseudomallei putative genes.
title_full_unstemmed Polymerase chain reaction and cloning of Burkholderia pseudomallei putative genes.
title_sort polymerase chain reaction and cloning of burkholderia pseudomallei putative genes.
publisher Medwell Publishing
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/16240/1/Polymerase%20chain%20reaction%20and%20cloning%20of%20Burkholderia%20pseudomallei%20putative%20genes.pdf
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score 13.4562235

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