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Predominance and emergence of clones of hospital-acquired methicillin-resistant Staphylococcus aureus in Malaysia.

We define the epidemiology of predominant and sporadic methicillin- resistant Staphylococcus aureus (MRSA) strains in a central teaching and referral hospital in Kuala Lumpur, Malaysia. This is done on the basis of spa sequencing, multilocus sequence typing (MLST), staphylococcal cassette chromosome...

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Hauptverfasser: Ghaznavi-Rad , Ehsanollah, Shamsudin, Mariana Nor, Sekawi, Zamberi, Liew , Y. K., Aziz, Mohammad Nazri, Hamat, Rukman Awang, Othman, Norlijah, Chong, Pei Pei, Van Belkum, Alex, Ghasemzadeh Moghaddam, Hamed, Vasanthakumari Kumari , Neela
Format: Artikel
Sprache:English
English
Veröffentlicht: American Society for Microbiology 2010
Schlagworte:
Staphylococcus aureus.
Methicillin resistance.
Community-Acquired Infections.
Online Zugang:http://psasir.upm.edu.my/id/eprint/16293/1/Predominance%20and%20emergence%20of%20clones%20of%20hospital.pdf
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spelling oai:psasir.upm.edu.my:16293 http://psasir.upm.edu.my/id/eprint/16293/ Predominance and emergence of clones of hospital-acquired methicillin-resistant Staphylococcus aureus in Malaysia. Ghaznavi-Rad , Ehsanollah Shamsudin, Mariana Nor Sekawi, Zamberi Liew , Y. K. Aziz, Mohammad Nazri Hamat, Rukman Awang Othman, Norlijah Chong, Pei Pei Van Belkum, Alex Ghasemzadeh Moghaddam, Hamed Vasanthakumari Kumari , Neela We define the epidemiology of predominant and sporadic methicillin- resistant Staphylococcus aureus (MRSA) strains in a central teaching and referral hospital in Kuala Lumpur, Malaysia. This is done on the basis of spa sequencing, multilocus sequence typing (MLST), staphylococcal cassette chromosome mec (SCCmec) typing, and virulence gene profiling. During the period of study, the MRSA prevalence was 44.1%, and 389 MRSA strains were included. The prevalence of MRSA was found to be significantly higher in the patients of Indian ethnicity (P < 0.001). The majority (92.5%) of the isolates belonged to ST-239, spa type t037, and possessed the type III or IIIA SCCmec. The arginine catabolic mobile element (ACME) arcA gene was detected in three (1.05%) ST-239 isolates. We report the first identification of ACME arcA gene-positive ST-239. Apart from this predominant clone, six (1.5%) isolates of ST-22, with two related spa types (t032 and t4184) and a singleton (t3213), carrying type IVh SCCmec, were detected for the first time in Asia. A limited number of community-acquired (CA) MRSA strains were also detected. These included ST-188/t189 (2.1%), ST-1/t127 (2.3%), and ST-7/t091 (1%). Panton-Valentin leukocidin (PVL) was detected in all ST-1 and ST-188 strains and in 0.7% of the ST-239 isolates. The majority of the isolates carried agr I, except that ST-1 strains were agr III positive. Virulence genes seg and sei were seen only among ST-22 isolates. In conclusion, current results revealed the predominance of ST-239-SCCmec III/IIIA and the penetration of ST-22 with different virulence gene profiles. The emergence in Malaysia of novel clones of known epidemic and pathogenic potential should be taken seriously. American Society for Microbiology 2010-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16293/1/Predominance%20and%20emergence%20of%20clones%20of%20hospital.pdf Ghaznavi-Rad , Ehsanollah and Shamsudin, Mariana Nor and Sekawi, Zamberi and Liew , Y. K. and Aziz, Mohammad Nazri and Hamat, Rukman Awang and Othman, Norlijah and Chong, Pei Pei and Van Belkum, Alex and Ghasemzadeh Moghaddam, Hamed and Vasanthakumari Kumari , Neela (2010) Predominance and emergence of clones of hospital-acquired methicillin-resistant Staphylococcus aureus in Malaysia. Journal of Clinical Microbiology, 48 (3). pp. 867-872. ISSN 0095-1137, ESSN: 1098-660X http://www.asm.org/ Staphylococcus aureus. Methicillin resistance. Community-Acquired Infections. 10.1128/JCM.01112-09 English
institution UPM IR
collection UPM IR
language English
English
topic Staphylococcus aureus.
Methicillin resistance.
Community-Acquired Infections.
spellingShingle Staphylococcus aureus.
Methicillin resistance.
Community-Acquired Infections.
Ghaznavi-Rad , Ehsanollah
Shamsudin, Mariana Nor
Sekawi, Zamberi
Liew , Y. K.
Aziz, Mohammad Nazri
Hamat, Rukman Awang
Othman, Norlijah
Chong, Pei Pei
Van Belkum, Alex
Ghasemzadeh Moghaddam, Hamed
Vasanthakumari Kumari , Neela
Predominance and emergence of clones of hospital-acquired methicillin-resistant Staphylococcus aureus in Malaysia.
description We define the epidemiology of predominant and sporadic methicillin- resistant Staphylococcus aureus (MRSA) strains in a central teaching and referral hospital in Kuala Lumpur, Malaysia. This is done on the basis of spa sequencing, multilocus sequence typing (MLST), staphylococcal cassette chromosome mec (SCCmec) typing, and virulence gene profiling. During the period of study, the MRSA prevalence was 44.1%, and 389 MRSA strains were included. The prevalence of MRSA was found to be significantly higher in the patients of Indian ethnicity (P < 0.001). The majority (92.5%) of the isolates belonged to ST-239, spa type t037, and possessed the type III or IIIA SCCmec. The arginine catabolic mobile element (ACME) arcA gene was detected in three (1.05%) ST-239 isolates. We report the first identification of ACME arcA gene-positive ST-239. Apart from this predominant clone, six (1.5%) isolates of ST-22, with two related spa types (t032 and t4184) and a singleton (t3213), carrying type IVh SCCmec, were detected for the first time in Asia. A limited number of community-acquired (CA) MRSA strains were also detected. These included ST-188/t189 (2.1%), ST-1/t127 (2.3%), and ST-7/t091 (1%). Panton-Valentin leukocidin (PVL) was detected in all ST-1 and ST-188 strains and in 0.7% of the ST-239 isolates. The majority of the isolates carried agr I, except that ST-1 strains were agr III positive. Virulence genes seg and sei were seen only among ST-22 isolates. In conclusion, current results revealed the predominance of ST-239-SCCmec III/IIIA and the penetration of ST-22 with different virulence gene profiles. The emergence in Malaysia of novel clones of known epidemic and pathogenic potential should be taken seriously.
format Article
author Ghaznavi-Rad , Ehsanollah
Shamsudin, Mariana Nor
Sekawi, Zamberi
Liew , Y. K.
Aziz, Mohammad Nazri
Hamat, Rukman Awang
Othman, Norlijah
Chong, Pei Pei
Van Belkum, Alex
Ghasemzadeh Moghaddam, Hamed
Vasanthakumari Kumari , Neela
author_facet Ghaznavi-Rad , Ehsanollah
Shamsudin, Mariana Nor
Sekawi, Zamberi
Liew , Y. K.
Aziz, Mohammad Nazri
Hamat, Rukman Awang
Othman, Norlijah
Chong, Pei Pei
Van Belkum, Alex
Ghasemzadeh Moghaddam, Hamed
Vasanthakumari Kumari , Neela
author_sort Ghaznavi-Rad , Ehsanollah
title Predominance and emergence of clones of hospital-acquired methicillin-resistant Staphylococcus aureus in Malaysia.
title_short Predominance and emergence of clones of hospital-acquired methicillin-resistant Staphylococcus aureus in Malaysia.
title_full Predominance and emergence of clones of hospital-acquired methicillin-resistant Staphylococcus aureus in Malaysia.
title_fullStr Predominance and emergence of clones of hospital-acquired methicillin-resistant Staphylococcus aureus in Malaysia.
title_full_unstemmed Predominance and emergence of clones of hospital-acquired methicillin-resistant Staphylococcus aureus in Malaysia.
title_sort predominance and emergence of clones of hospital-acquired methicillin-resistant staphylococcus aureus in malaysia.
publisher American Society for Microbiology
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/16293/1/Predominance%20and%20emergence%20of%20clones%20of%20hospital.pdf
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score 13.4562235

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