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Reduction of molybdate to molybdenum blue by Enterobacter sp. strain DRY13.

Extensive use of metals in various industrial applications has caused substantial environmental pollution. Molybdenum-reducing bacteria isolated from soils can be used to remove molybdenum from contaminated environments. In this work we have isolated a local bacterium with the capability to reduce s...

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Main Authors: Abd. Shukor , Mohd. Yunus, Shamaan, Nor Aripin, Syed, Mohd. Arif
Format: Article
Language:English
English
Published: Wiley-VCH 2009
Subjects:
Molybdate.
Molybdenum - Analysis.
Molybdenum - Environmental aspects.
Online Access:http://psasir.upm.edu.my/id/eprint/16463/1/Reduction%20of%20molybdate%20to%20molybdenum%20blue%20by%20Enterobacter%20sp.pdf
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spelling oai:psasir.upm.edu.my:16463 http://psasir.upm.edu.my/id/eprint/16463/ Reduction of molybdate to molybdenum blue by Enterobacter sp. strain DRY13. Abd. Shukor , Mohd. Yunus Shamaan, Nor Aripin Syed, Mohd. Arif Extensive use of metals in various industrial applications has caused substantial environmental pollution. Molybdenum-reducing bacteria isolated from soils can be used to remove molybdenum from contaminated environments. In this work we have isolated a local bacterium with the capability to reduce soluble molybdate to the insoluble molybdenum blue. We studied several factors that would optimize molybdate reduction. Electron donor sources such as glucose, sucrose, lactose, maltose and fructose (in decreasing efficiency) supported molybdate reduction after 24 h of incubation with optimum glucose concentration for molybdate reduction at 1.5% (w/v). The optimum pH, phosphate and molybdate concentrations, and temperature for molybdate reduction were pH 6.5, 5.0, 25 to 50 mM and 37 degrees C, respectively. The Mo-blue produced by cellular reduction exhibited a unique absorption spectrum with a maximum peak at 865 nm and a shoulder at 700 nm. Metal ions such as chromium, cadmium, copper, silver and mercury caused approximately 73, 71, 81, 77 and 78% inhibition of the molybdenum-reducing activity, respectively. All of the respiratory inhibitors tested namely rotenone, azide, cyanide and antimycin A did not show any inhibition to the molybdenum-reducing activity suggesting components of the electron transport system are not responsible for the reducing activity. The isolate was tentatively identified as Enterobacter sp. strain Dr.Y13 based on carbon utilization profiles using Biolog GN plates and partial 16S rDNA molecular phylogeny. Wiley-VCH 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16463/1/Reduction%20of%20molybdate%20to%20molybdenum%20blue%20by%20Enterobacter%20sp.pdf Abd. Shukor , Mohd. Yunus and Shamaan, Nor Aripin and Syed, Mohd. Arif (2009) Reduction of molybdate to molybdenum blue by Enterobacter sp. strain DRY13. Journal of Basic Microbiology, 49 (1). pp. 43-54. ISSN 0233-111X Molybdate. Molybdenum - Analysis. Molybdenum - Environmental aspects. English
institution UPM IR
collection UPM IR
language English
English
topic Molybdate.
Molybdenum - Analysis.
Molybdenum - Environmental aspects.
spellingShingle Molybdate.
Molybdenum - Analysis.
Molybdenum - Environmental aspects.
Abd. Shukor , Mohd. Yunus
Shamaan, Nor Aripin
Syed, Mohd. Arif
Reduction of molybdate to molybdenum blue by Enterobacter sp. strain DRY13.
description Extensive use of metals in various industrial applications has caused substantial environmental pollution. Molybdenum-reducing bacteria isolated from soils can be used to remove molybdenum from contaminated environments. In this work we have isolated a local bacterium with the capability to reduce soluble molybdate to the insoluble molybdenum blue. We studied several factors that would optimize molybdate reduction. Electron donor sources such as glucose, sucrose, lactose, maltose and fructose (in decreasing efficiency) supported molybdate reduction after 24 h of incubation with optimum glucose concentration for molybdate reduction at 1.5% (w/v). The optimum pH, phosphate and molybdate concentrations, and temperature for molybdate reduction were pH 6.5, 5.0, 25 to 50 mM and 37 degrees C, respectively. The Mo-blue produced by cellular reduction exhibited a unique absorption spectrum with a maximum peak at 865 nm and a shoulder at 700 nm. Metal ions such as chromium, cadmium, copper, silver and mercury caused approximately 73, 71, 81, 77 and 78% inhibition of the molybdenum-reducing activity, respectively. All of the respiratory inhibitors tested namely rotenone, azide, cyanide and antimycin A did not show any inhibition to the molybdenum-reducing activity suggesting components of the electron transport system are not responsible for the reducing activity. The isolate was tentatively identified as Enterobacter sp. strain Dr.Y13 based on carbon utilization profiles using Biolog GN plates and partial 16S rDNA molecular phylogeny.
format Article
author Abd. Shukor , Mohd. Yunus
Shamaan, Nor Aripin
Syed, Mohd. Arif
author_facet Abd. Shukor , Mohd. Yunus
Shamaan, Nor Aripin
Syed, Mohd. Arif
author_sort Abd. Shukor , Mohd. Yunus
title Reduction of molybdate to molybdenum blue by Enterobacter sp. strain DRY13.
title_short Reduction of molybdate to molybdenum blue by Enterobacter sp. strain DRY13.
title_full Reduction of molybdate to molybdenum blue by Enterobacter sp. strain DRY13.
title_fullStr Reduction of molybdate to molybdenum blue by Enterobacter sp. strain DRY13.
title_full_unstemmed Reduction of molybdate to molybdenum blue by Enterobacter sp. strain DRY13.
title_sort reduction of molybdate to molybdenum blue by enterobacter sp. strain dry13.
publisher Wiley-VCH
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/16463/1/Reduction%20of%20molybdate%20to%20molybdenum%20blue%20by%20Enterobacter%20sp.pdf
_version_ 1819294031332507648
score 13.4562235

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