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Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss

Mitochondrial DNA mutations play an important role in causing sensorineural hearing loss. The purpose of this study was to determine the association of the mitochondrial genes RNR1, MT-TL1, and ND1 as well as the nuclear genes GJB2 and GJB6 with audiological examinations in nonfamilial Iranians with...

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Main Authors: Balali, Maryam, Kamalidehghan, Behnam, Farhadi, Mohammad, Ahmadipour, Fatemeh, Ashkezari, Mahmoud Dehghani, Hemami, Mohsen Rezaei, Arabzadeh, Hossein, Falah, Masoumeh, Goh, Yong Meng, Houshmand, Massoud
Format: Article
Language:English
Published: Dove Medical Press 2016
Online Access:http://psasir.upm.edu.my/id/eprint/16708/1/16708.pdf
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spelling oai:psasir.upm.edu.my:16708 http://psasir.upm.edu.my/id/eprint/16708/ Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss Balali, Maryam Kamalidehghan, Behnam Farhadi, Mohammad Ahmadipour, Fatemeh Ashkezari, Mahmoud Dehghani Hemami, Mohsen Rezaei Arabzadeh, Hossein Falah, Masoumeh Goh, Yong Meng Houshmand, Massoud Mitochondrial DNA mutations play an important role in causing sensorineural hearing loss. The purpose of this study was to determine the association of the mitochondrial genes RNR1, MT-TL1, and ND1 as well as the nuclear genes GJB2 and GJB6 with audiological examinations in nonfamilial Iranians with cochlear implants, using polymerase chain reaction, DNA sequencing, and RNA secondary structure analysis. We found that there were no novel mutations in the mitochondrial gene 12S rRNA (MT-RNR1) in patients with and without GJB2 mutation (GJB2+ and GJB2-, respectively), but a total of six polymorphisms were found. No mutations were observed in tRNALeu(UUR) (MT-TL1). Furthermore, eight polymorphisms were found in the mitochondrial ND1 gene. Additionally, no mutations were observed in the nuclear GJB6 gene in patients in the GJB2- and GJB2+ groups. The speech intelligibility rating and category of auditory perception tests were statistically assessed in patients in the GJB2- and GJB2+ groups. The results indicated that there was a significant difference (P<0.05) between the categories of auditory perception score in the GJB2- group compared to that in the GJB2+ group. Successful cochlear implantation was observed among individuals with GJB2 mutations (GJB2+) and mitochondrial polymorphisms compared to those without GJB2 mutations (GJB2-). In conclusion, the outcome of this study suggests that variation in the mitochondrial and nuclear genes may influence the penetrance of deafness. Therefore, further genetic and functional studies are required to help patients in making the best choice for cochlear implants. Dove Medical Press 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16708/1/16708.pdf Balali, Maryam and Kamalidehghan, Behnam and Farhadi, Mohammad and Ahmadipour, Fatemeh and Ashkezari, Mahmoud Dehghani and Hemami, Mohsen Rezaei and Arabzadeh, Hossein and Falah, Masoumeh and Goh, Yong Meng and Houshmand, Massoud (2016) Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss. Therapeutics and Clinical Risk Management, 12. pp. 117-128. ISSN 1176-6336; ESSN: 1178-203X https://www.dovepress.com/association-of-nuclear-and-mitochondrial-genes-with-audiological-exami-peer-reviewed-article-TCRM 10.2147/TCRM.S90581
institution UPM IR
collection UPM IR
language English
description Mitochondrial DNA mutations play an important role in causing sensorineural hearing loss. The purpose of this study was to determine the association of the mitochondrial genes RNR1, MT-TL1, and ND1 as well as the nuclear genes GJB2 and GJB6 with audiological examinations in nonfamilial Iranians with cochlear implants, using polymerase chain reaction, DNA sequencing, and RNA secondary structure analysis. We found that there were no novel mutations in the mitochondrial gene 12S rRNA (MT-RNR1) in patients with and without GJB2 mutation (GJB2+ and GJB2-, respectively), but a total of six polymorphisms were found. No mutations were observed in tRNALeu(UUR) (MT-TL1). Furthermore, eight polymorphisms were found in the mitochondrial ND1 gene. Additionally, no mutations were observed in the nuclear GJB6 gene in patients in the GJB2- and GJB2+ groups. The speech intelligibility rating and category of auditory perception tests were statistically assessed in patients in the GJB2- and GJB2+ groups. The results indicated that there was a significant difference (P<0.05) between the categories of auditory perception score in the GJB2- group compared to that in the GJB2+ group. Successful cochlear implantation was observed among individuals with GJB2 mutations (GJB2+) and mitochondrial polymorphisms compared to those without GJB2 mutations (GJB2-). In conclusion, the outcome of this study suggests that variation in the mitochondrial and nuclear genes may influence the penetrance of deafness. Therefore, further genetic and functional studies are required to help patients in making the best choice for cochlear implants.
format Article
author Balali, Maryam
Kamalidehghan, Behnam
Farhadi, Mohammad
Ahmadipour, Fatemeh
Ashkezari, Mahmoud Dehghani
Hemami, Mohsen Rezaei
Arabzadeh, Hossein
Falah, Masoumeh
Goh, Yong Meng
Houshmand, Massoud
spellingShingle Balali, Maryam
Kamalidehghan, Behnam
Farhadi, Mohammad
Ahmadipour, Fatemeh
Ashkezari, Mahmoud Dehghani
Hemami, Mohsen Rezaei
Arabzadeh, Hossein
Falah, Masoumeh
Goh, Yong Meng
Houshmand, Massoud
Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss
author_facet Balali, Maryam
Kamalidehghan, Behnam
Farhadi, Mohammad
Ahmadipour, Fatemeh
Ashkezari, Mahmoud Dehghani
Hemami, Mohsen Rezaei
Arabzadeh, Hossein
Falah, Masoumeh
Goh, Yong Meng
Houshmand, Massoud
author_sort Balali, Maryam
title Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss
title_short Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss
title_full Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss
title_fullStr Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss
title_full_unstemmed Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss
title_sort association of nuclear and mitochondrial genes with audiological examinations in iranian patients with nonaminoglycoside antibiotics-induced hearing loss
publisher Dove Medical Press
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/16708/1/16708.pdf
_version_ 1819294057958998016
score 13.4562235

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