Synthesis, characterization, and structural properties of intracellular copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB 172 from renewable resource
Microbial copolymer was produced by a local isolate, Comamonas sp. EB 172, using mixed organic acids such as acetic, propionic, and butyric acids as carbon sources in pH-stat fed-batch fermentation. Maximum polymer production (6.59 g/L) was achieved at 50 h of fermentation when 73.64 g/L mixed acids...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis
2010
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/16956/1/Synthesis%2C%20characterization%20and%20structural%20properties%20of%20intracellular%20copolyester%20poly%283-hydroxybutyrate-co-%203-%20hydroxyvalerate%29%20produced%20by%20Comamonas%20sp.%20EB%20172%20from%20renewable%20resource..pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Microbial copolymer was produced by a local isolate, Comamonas sp. EB 172, using mixed organic acids such as acetic, propionic, and butyric acids as carbon sources in pH-stat fed-batch fermentation. Maximum polymer production (6.59 g/L) was achieved at 50 h of fermentation when 73.64 g/L mixed acids, generated from the acidogenic fermentation of palm oil mill wastewater, were used. Accumulation of polymer in the cell was 70% (wt/wt), which was observed under transmission electron microscope. The morphological, chemical, thermal, and mechanical properties of the solvent-extracted biopolymer were determined by various techniques (SEM, GC, 13C NMR, FT-IR, TGA, and tensile testing). The copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) consisted of 87 mol% β-hydroxybutyric acid (HB) and 13 mol% β-hydroxyvaleric acid (HV). With chemical properties similar to commercial PHBV and mechanical strength of around 30 MPa and 8% elongation at break, the biopolymer offers potential for industrial applications. |
|---|