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Molecular differentiation of infectious Bursal Disease Virus IBDV) strains.

This invention relates to the detection and distinguishing Infectious Bursal Disease Virus (IBDV) strains by a fluorescent probe based real-time polymerase chain reaction in chicken or other birds. More particularly, this invention relates to distinguishing different Infectious Bursal Diseases Virus...

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Egile Nagusiak: Omar, Abdul Rahman, Bejo, Mohd Hair, Ideris, Aini, Kong, Lih Ling
Formatua: Patent
Hizkuntza:English
Argitaratua: 2012
Etiketak: Etiketa erantsi
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id oai:psasir.upm.edu.my:20567
record_format eprints
spelling oai:psasir.upm.edu.my:20567 http://psasir.upm.edu.my/id/eprint/20567/ Molecular differentiation of infectious Bursal Disease Virus IBDV) strains. Omar, Abdul Rahman Bejo, Mohd Hair Ideris, Aini Kong, Lih Ling This invention relates to the detection and distinguishing Infectious Bursal Disease Virus (IBDV) strains by a fluorescent probe based real-time polymerase chain reaction in chicken or other birds. More particularly, this invention relates to distinguishing different Infectious Bursal Diseases Virus (IBDV) strains in chicken and other bird sample by novel subtype specific primers and fluorescent probe based on one-tube duplex Real-time Polymerase Chain Reaction (PCR) method. The PCR conditions are optimized in order to obtain optimum PCR parameters on the ingredients and profile using samples containing IBDV RNA in a Taqman probe based duplex real-time PCR. Hence, for differentiation of very virulent from vaccine strains of IBDV by using Primer FWDC (nucleotide position: 2084 to 2102) and RVSC (nucleotide position: 2178 to 2197) were designed from the conserved region of VP4 of both very virulent and classical strains, respectively, to generate a 114 bp amplicon. A dual-labeled fluorescent probe FAM 5-TAMRA 3, (nucleotide position: 2112 to 2133), was designed with the sequence specific to aligned very virulent IBDV strains (Probe 1), and a second dual-labeled fluorescent probe HEX 5 - TAMRA 3, (nucleotide position: 2112 to 2133), was designed with the sequence specific to aligned classical IBDV strains (Probe 2). 2012-08-15 Patent NonPeerReviewed Abdul Rahman Omar (2012) Molecular differentiation of infectious Bursal Disease Virus IBDV) strains. UNSPECIFIED. English
institution UPM IR
collection UPM IR
language English
description This invention relates to the detection and distinguishing Infectious Bursal Disease Virus (IBDV) strains by a fluorescent probe based real-time polymerase chain reaction in chicken or other birds. More particularly, this invention relates to distinguishing different Infectious Bursal Diseases Virus (IBDV) strains in chicken and other bird sample by novel subtype specific primers and fluorescent probe based on one-tube duplex Real-time Polymerase Chain Reaction (PCR) method. The PCR conditions are optimized in order to obtain optimum PCR parameters on the ingredients and profile using samples containing IBDV RNA in a Taqman probe based duplex real-time PCR. Hence, for differentiation of very virulent from vaccine strains of IBDV by using Primer FWDC (nucleotide position: 2084 to 2102) and RVSC (nucleotide position: 2178 to 2197) were designed from the conserved region of VP4 of both very virulent and classical strains, respectively, to generate a 114 bp amplicon. A dual-labeled fluorescent probe FAM 5-TAMRA 3, (nucleotide position: 2112 to 2133), was designed with the sequence specific to aligned very virulent IBDV strains (Probe 1), and a second dual-labeled fluorescent probe HEX 5 - TAMRA 3, (nucleotide position: 2112 to 2133), was designed with the sequence specific to aligned classical IBDV strains (Probe 2).
format Patent
author Omar, Abdul Rahman
Bejo, Mohd Hair
Ideris, Aini
Kong, Lih Ling
spellingShingle Omar, Abdul Rahman
Bejo, Mohd Hair
Ideris, Aini
Kong, Lih Ling
Molecular differentiation of infectious Bursal Disease Virus IBDV) strains.
author_facet Omar, Abdul Rahman
Bejo, Mohd Hair
Ideris, Aini
Kong, Lih Ling
author_sort Omar, Abdul Rahman
title Molecular differentiation of infectious Bursal Disease Virus IBDV) strains.
title_short Molecular differentiation of infectious Bursal Disease Virus IBDV) strains.
title_full Molecular differentiation of infectious Bursal Disease Virus IBDV) strains.
title_fullStr Molecular differentiation of infectious Bursal Disease Virus IBDV) strains.
title_full_unstemmed Molecular differentiation of infectious Bursal Disease Virus IBDV) strains.
title_sort molecular differentiation of infectious bursal disease virus ibdv) strains.
publishDate 2012
_version_ 1819294402494857216
score 13.4562235

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