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Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system.

In this works, the polymer–salt aqueous two phase system (ATPS) which is polyethylene–glycol (PEG) with sodium citrate was constructed to purify the enzyme CGTase from fermentation broth. Impacts of parameters such as phase composition, tie-line length (TLL), volume ratio (VR), crude sample loading,...

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Main Authors: Ng, Hui Suan, Tan, Chin Ping, Chen, Soo Kien, Mokhtar, Mohd Noriznan, Ariff, Arbakariya, Ling, Tau Chuan
Format: Article
Language:English
English
Published: Elsevier 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22298/1/Primary%20capture%20of%20cyclodextrin%20glycosyltransferase%20derived%20from%20Bacillus%20cereus%20by%20aqueous%20two%20phase%20system.pdf
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spelling oai:psasir.upm.edu.my:22298 http://psasir.upm.edu.my/id/eprint/22298/ Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system. Ng, Hui Suan Tan, Chin Ping Chen, Soo Kien Mokhtar, Mohd Noriznan Ariff, Arbakariya Ling, Tau Chuan In this works, the polymer–salt aqueous two phase system (ATPS) which is polyethylene–glycol (PEG) with sodium citrate was constructed to purify the enzyme CGTase from fermentation broth. Impacts of parameters such as phase composition, tie-line length (TLL), volume ratio (VR), crude sample loading, pH and the addition of sodium chloride (NaCl) on the partition behavior of cyclodextrin glycosyltransferase (CGTase) were investigated. The study exhibited that the optimum system for the purification of the enzyme CGTase was achieved on the 19.0% PEG and 11.5% citrate system with TLL of 38.89% (w/w), VR of 2.0, with additional 4% (w/w) NaCl and 20% crude load at pH 7.0. CGTase from Bacillus sp. was partially purified by the ATPS up to 16.3-fold with a yield of 70%. Elsevier 2011-10-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22298/1/Primary%20capture%20of%20cyclodextrin%20glycosyltransferase%20derived%20from%20Bacillus%20cereus%20by%20aqueous%20two%20phase%20system.pdf Ng, Hui Suan and Tan, Chin Ping and Chen, Soo Kien and Mokhtar, Mohd Noriznan and Ariff, Arbakariya and Ling, Tau Chuan (2011) Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system. Separation and Purification Technology, 81 (3). pp. 318-324. ISSN 1383-5866 10.1016/j.seppur.2011.07.039 English
institution UPM IR
collection UPM IR
language English
English
description In this works, the polymer–salt aqueous two phase system (ATPS) which is polyethylene–glycol (PEG) with sodium citrate was constructed to purify the enzyme CGTase from fermentation broth. Impacts of parameters such as phase composition, tie-line length (TLL), volume ratio (VR), crude sample loading, pH and the addition of sodium chloride (NaCl) on the partition behavior of cyclodextrin glycosyltransferase (CGTase) were investigated. The study exhibited that the optimum system for the purification of the enzyme CGTase was achieved on the 19.0% PEG and 11.5% citrate system with TLL of 38.89% (w/w), VR of 2.0, with additional 4% (w/w) NaCl and 20% crude load at pH 7.0. CGTase from Bacillus sp. was partially purified by the ATPS up to 16.3-fold with a yield of 70%.
format Article
author Ng, Hui Suan
Tan, Chin Ping
Chen, Soo Kien
Mokhtar, Mohd Noriznan
Ariff, Arbakariya
Ling, Tau Chuan
spellingShingle Ng, Hui Suan
Tan, Chin Ping
Chen, Soo Kien
Mokhtar, Mohd Noriznan
Ariff, Arbakariya
Ling, Tau Chuan
Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system.
author_facet Ng, Hui Suan
Tan, Chin Ping
Chen, Soo Kien
Mokhtar, Mohd Noriznan
Ariff, Arbakariya
Ling, Tau Chuan
author_sort Ng, Hui Suan
title Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system.
title_short Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system.
title_full Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system.
title_fullStr Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system.
title_full_unstemmed Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system.
title_sort primary capture of cyclodextrin glycosyltransferase derived from bacillus cereus by aqueous two phase system.
publisher Elsevier
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/22298/1/Primary%20capture%20of%20cyclodextrin%20glycosyltransferase%20derived%20from%20Bacillus%20cereus%20by%20aqueous%20two%20phase%20system.pdf
_version_ 1819294555000799232
score 13.4562235

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