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Effect of retention time on biohydrogen production by microbial consortia immbolised in polydimethylsiloxane

An investigation on biohydrogen production from palm oil mill effluent (POME) was conducted in a continuous stirred tank reactor seeded with polydimethyl-siloxane (PDMS) immobilised mixed cultures at adjusted retention time. The hydrogen-producing bacteria obtained from an anaerobic digester used fo...

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Main Authors: Ismail, Isnazunita, Hassan, Mohd Ali, Abdul Rahman, Nor'aini, Chen, Sau Soon
Format: Article
Language:English
Published: Academic Journals 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22361/1/Effect%20of%20retention%20time%20on%20biohydrogen%20production%20by%20microbial%20consortia%20immbolised%20in%20polydimethylsiloxane.pdf
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spelling oai:psasir.upm.edu.my:22361 http://psasir.upm.edu.my/id/eprint/22361/ Effect of retention time on biohydrogen production by microbial consortia immbolised in polydimethylsiloxane Ismail, Isnazunita Hassan, Mohd Ali Abdul Rahman, Nor'aini Chen, Sau Soon An investigation on biohydrogen production from palm oil mill effluent (POME) was conducted in a continuous stirred tank reactor seeded with polydimethyl-siloxane (PDMS) immobilised mixed cultures at adjusted retention time. The hydrogen-producing bacteria obtained from an anaerobic digester used for treating POME were acclimatised and immobilised in PDMS. The immobilised cultures were assessed for their effectiveness in generating hydrogen in a continuous system. In this study, the PDMS cultures were fed with raw POME at hydraulic retention times of 6, 4 and 2 days and operated at controlled pH and temperature of 5.5 and 55°C, respectively. At hydraulic retention time (HRT) 2 days, the average hydrogen production rate per unit volume of POME was 2.1 NL/L/d. Hydrogen constituted up to 43% of the total gas produced and methane was not detected throughout the 150 days of continuous operation. The soluble carbohydrate degradation efficiency was highest at 81.2% during HRT 4 days and the concentration of soluble metabolites produced, followed the order of acetic > butyric > ethanol > propionic acid. The microbial diversity of the immobilised consortia determined by denaturing gradient gel electrophoresis (DGGE) changed at different HRTs; with increasing dominant species phylogenetically related to Clostridaceae. Academic Journals 2011-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22361/1/Effect%20of%20retention%20time%20on%20biohydrogen%20production%20by%20microbial%20consortia%20immbolised%20in%20polydimethylsiloxane.pdf Ismail, Isnazunita and Hassan, Mohd Ali and Abdul Rahman, Nor'aini and Chen, Sau Soon (2011) Effect of retention time on biohydrogen production by microbial consortia immbolised in polydimethylsiloxane. African Journal of Biotechnology, 10 (4 ). art. no. C7081B027236. pp. 601-609. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/C7081B027236
institution UPM IR
collection UPM IR
language English
description An investigation on biohydrogen production from palm oil mill effluent (POME) was conducted in a continuous stirred tank reactor seeded with polydimethyl-siloxane (PDMS) immobilised mixed cultures at adjusted retention time. The hydrogen-producing bacteria obtained from an anaerobic digester used for treating POME were acclimatised and immobilised in PDMS. The immobilised cultures were assessed for their effectiveness in generating hydrogen in a continuous system. In this study, the PDMS cultures were fed with raw POME at hydraulic retention times of 6, 4 and 2 days and operated at controlled pH and temperature of 5.5 and 55°C, respectively. At hydraulic retention time (HRT) 2 days, the average hydrogen production rate per unit volume of POME was 2.1 NL/L/d. Hydrogen constituted up to 43% of the total gas produced and methane was not detected throughout the 150 days of continuous operation. The soluble carbohydrate degradation efficiency was highest at 81.2% during HRT 4 days and the concentration of soluble metabolites produced, followed the order of acetic > butyric > ethanol > propionic acid. The microbial diversity of the immobilised consortia determined by denaturing gradient gel electrophoresis (DGGE) changed at different HRTs; with increasing dominant species phylogenetically related to Clostridaceae.
format Article
author Ismail, Isnazunita
Hassan, Mohd Ali
Abdul Rahman, Nor'aini
Chen, Sau Soon
spellingShingle Ismail, Isnazunita
Hassan, Mohd Ali
Abdul Rahman, Nor'aini
Chen, Sau Soon
Effect of retention time on biohydrogen production by microbial consortia immbolised in polydimethylsiloxane
author_facet Ismail, Isnazunita
Hassan, Mohd Ali
Abdul Rahman, Nor'aini
Chen, Sau Soon
author_sort Ismail, Isnazunita
title Effect of retention time on biohydrogen production by microbial consortia immbolised in polydimethylsiloxane
title_short Effect of retention time on biohydrogen production by microbial consortia immbolised in polydimethylsiloxane
title_full Effect of retention time on biohydrogen production by microbial consortia immbolised in polydimethylsiloxane
title_fullStr Effect of retention time on biohydrogen production by microbial consortia immbolised in polydimethylsiloxane
title_full_unstemmed Effect of retention time on biohydrogen production by microbial consortia immbolised in polydimethylsiloxane
title_sort effect of retention time on biohydrogen production by microbial consortia immbolised in polydimethylsiloxane
publisher Academic Journals
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/22361/1/Effect%20of%20retention%20time%20on%20biohydrogen%20production%20by%20microbial%20consortia%20immbolised%20in%20polydimethylsiloxane.pdf
_version_ 1819294561425424384
score 13.4562235

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