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'Heat-treatment aqueous two phase system' for purification of serine protease from kesinai (Streblus asper) leaves

A ‘Heat treatment aqueous two phase system’ was employed for the first time to purify serine protease from kesinai (Streblus asper) leaves. In this study, introduction of heat treatment procedure in serine protease purification was investigated. In addition, the effects of different molecular weight...

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Hoofdauteurs: Amid, Mehrnoush, Mustafa, Shuhaimi, Abdul Manap, Mohd Yazid
Formaat: Artikel
Taal:English
Gepubliceerd in: MDPI 2011
Online toegang:http://psasir.upm.edu.my/id/eprint/22364/1/Heat-treatment%20aqueous%20two%20phase%20system%20for%20purification%20of%20serine%20protease%20from%20Kesinai%20%28Streblus%20asper%29%20leaves.pdf
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spelling oai:psasir.upm.edu.my:22364 http://psasir.upm.edu.my/id/eprint/22364/ 'Heat-treatment aqueous two phase system' for purification of serine protease from kesinai (Streblus asper) leaves Amid, Mehrnoush Mustafa, Shuhaimi Abdul Manap, Mohd Yazid A ‘Heat treatment aqueous two phase system’ was employed for the first time to purify serine protease from kesinai (Streblus asper) leaves. In this study, introduction of heat treatment procedure in serine protease purification was investigated. In addition, the effects of different molecular weights of polyethylene glycol (PEG 4000, 6000 and 8000) at concentrations of 8, 16 and 21% (w/w) as well as salts (Na-citrate, MgSO4 and K2HPO4) at concentrations of 12, 15, 18% (w/w) on serine protease partition behavior were studied. Optimum conditions for serine protease purification were achieved in the PEG-rich phase with composition of 16% PEG6000-15% MgSO4. Also, thermal treatment of kesinai leaves at 55 °C for 15 min resulted in higher purity and recovery yield compared to the non-heat treatment sample. Furthermore, this study investigated the effects of various concentrations of NaCl addition (2, 4, 6 and 8% w/w) and different pH (4, 7 and 9) on the optimization of the system to obtain high yields of the enzyme. The recovery of serine protease was significantly enhanced in the presence of 4% (w/w) of NaCl at pH 7.0. Based on this system, the purification factor was increased 14.4 fold and achieved a high yield of 96.7%. MDPI 2011-12-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22364/1/Heat-treatment%20aqueous%20two%20phase%20system%20for%20purification%20of%20serine%20protease%20from%20Kesinai%20%28Streblus%20asper%29%20leaves.pdf Amid, Mehrnoush and Mustafa, Shuhaimi and Abdul Manap, Mohd Yazid (2011) 'Heat-treatment aqueous two phase system' for purification of serine protease from kesinai (Streblus asper) leaves. Molecules, 16 (12). pp. 10202-10213. ISSN 1420-3049 http://www.mdpi.com/1420-3049/16/12/10202 10.3390/molecules161210202
institution UPM IR
collection UPM IR
language English
description A ‘Heat treatment aqueous two phase system’ was employed for the first time to purify serine protease from kesinai (Streblus asper) leaves. In this study, introduction of heat treatment procedure in serine protease purification was investigated. In addition, the effects of different molecular weights of polyethylene glycol (PEG 4000, 6000 and 8000) at concentrations of 8, 16 and 21% (w/w) as well as salts (Na-citrate, MgSO4 and K2HPO4) at concentrations of 12, 15, 18% (w/w) on serine protease partition behavior were studied. Optimum conditions for serine protease purification were achieved in the PEG-rich phase with composition of 16% PEG6000-15% MgSO4. Also, thermal treatment of kesinai leaves at 55 °C for 15 min resulted in higher purity and recovery yield compared to the non-heat treatment sample. Furthermore, this study investigated the effects of various concentrations of NaCl addition (2, 4, 6 and 8% w/w) and different pH (4, 7 and 9) on the optimization of the system to obtain high yields of the enzyme. The recovery of serine protease was significantly enhanced in the presence of 4% (w/w) of NaCl at pH 7.0. Based on this system, the purification factor was increased 14.4 fold and achieved a high yield of 96.7%.
format Article
author Amid, Mehrnoush
Mustafa, Shuhaimi
Abdul Manap, Mohd Yazid
spellingShingle Amid, Mehrnoush
Mustafa, Shuhaimi
Abdul Manap, Mohd Yazid
'Heat-treatment aqueous two phase system' for purification of serine protease from kesinai (Streblus asper) leaves
author_facet Amid, Mehrnoush
Mustafa, Shuhaimi
Abdul Manap, Mohd Yazid
author_sort Amid, Mehrnoush
title 'Heat-treatment aqueous two phase system' for purification of serine protease from kesinai (Streblus asper) leaves
title_short 'Heat-treatment aqueous two phase system' for purification of serine protease from kesinai (Streblus asper) leaves
title_full 'Heat-treatment aqueous two phase system' for purification of serine protease from kesinai (Streblus asper) leaves
title_fullStr 'Heat-treatment aqueous two phase system' for purification of serine protease from kesinai (Streblus asper) leaves
title_full_unstemmed 'Heat-treatment aqueous two phase system' for purification of serine protease from kesinai (Streblus asper) leaves
title_sort 'heat-treatment aqueous two phase system' for purification of serine protease from kesinai (streblus asper) leaves
publisher MDPI
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/22364/1/Heat-treatment%20aqueous%20two%20phase%20system%20for%20purification%20of%20serine%20protease%20from%20Kesinai%20%28Streblus%20asper%29%20leaves.pdf
_version_ 1819294561716928512
score 13.4562235

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