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Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells.

Background: Mesenchymal stem cells (MSC) have great potential in regenerative medicine, immunotherapy and gene therapy due to their unique properties of self-renewal, high plasticity, immune modulation and ease for genetic modification. However, production of MSC at sufficient clinical scale remains...

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Main Authors: Ramasamy, Rajesh, Tong, C. K., Yip, W. K., Vellasamy, S., Tan, B. C., Seow, Heng Fong
Format: Article
Language:English
English
Published: Wiley-Blackwell 2012
Online Access:http://psasir.upm.edu.my/id/eprint/24714/1/Basic%20fibroblast%20growth%20factor%20modulates%20cell%20cycle%20of%20human%20umbilical%20cord.pdf
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spelling oai:psasir.upm.edu.my:24714 http://psasir.upm.edu.my/id/eprint/24714/ Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells. Ramasamy, Rajesh Tong, C. K. Yip, W. K. Vellasamy, S. Tan, B. C. Seow, Heng Fong Background: Mesenchymal stem cells (MSC) have great potential in regenerative medicine, immunotherapy and gene therapy due to their unique properties of self-renewal, high plasticity, immune modulation and ease for genetic modification. However, production of MSC at sufficient clinical scale remains an issue as in vitro generation of MSC inadequately fulfils the demand with respect to patients. Objectives: This study has aimed to establish optimum conditions to generate and characterize MSC from human umbilical cord (UC-MSC). Materials and methods: To optimize MSC population growth, basic fibroblast growth factor (bFGF) was utilized in culture media. Effects of bFGF on expansion kinetics, cell cycle, survival of UC-MSC, cytokine secretion, expression of early stem-cell markers and immunomodulation were investigated. Results: bFGF supplementation profoundly enhanced UC-MSC proliferation by reducing population doubling time without altering immunophenotype and immunomodulatory function of UC-MSC. However, cell cycle studies revealed that bFGF drove the cells into the cell cycle, as a higher proportion of cells resided in S phase and progressed into M phase. Consistent with this, bFGF was shown to promote expression of cyclin D proteins and their relevant kinases to drive UC-MSC to transverse cell cycle check points, thus, committing the cells to DNA synthesis. Furthermore, supplementation with bFGF changed the cytokine profiles of the cells and reduced their apoptotic level. Conclusion: Our study showed that bFGF supplementation of UC-MSC culture enhanced the cells' growth kinetics without compromising their nature. Wiley-Blackwell 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24714/1/Basic%20fibroblast%20growth%20factor%20modulates%20cell%20cycle%20of%20human%20umbilical%20cord.pdf Ramasamy, Rajesh and Tong, C. K. and Yip, W. K. and Vellasamy, S. and Tan, B. C. and Seow, Heng Fong (2012) Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells. Cell Proliferation , 45 (2). pp. 132-139. ISSN 0960-7722; ESSN:1365-2184 http://as.wiley.com/ 10.1111/j.1365-2184.2012.00808.x English
institution UPM IR
collection UPM IR
language English
English
description Background: Mesenchymal stem cells (MSC) have great potential in regenerative medicine, immunotherapy and gene therapy due to their unique properties of self-renewal, high plasticity, immune modulation and ease for genetic modification. However, production of MSC at sufficient clinical scale remains an issue as in vitro generation of MSC inadequately fulfils the demand with respect to patients. Objectives: This study has aimed to establish optimum conditions to generate and characterize MSC from human umbilical cord (UC-MSC). Materials and methods: To optimize MSC population growth, basic fibroblast growth factor (bFGF) was utilized in culture media. Effects of bFGF on expansion kinetics, cell cycle, survival of UC-MSC, cytokine secretion, expression of early stem-cell markers and immunomodulation were investigated. Results: bFGF supplementation profoundly enhanced UC-MSC proliferation by reducing population doubling time without altering immunophenotype and immunomodulatory function of UC-MSC. However, cell cycle studies revealed that bFGF drove the cells into the cell cycle, as a higher proportion of cells resided in S phase and progressed into M phase. Consistent with this, bFGF was shown to promote expression of cyclin D proteins and their relevant kinases to drive UC-MSC to transverse cell cycle check points, thus, committing the cells to DNA synthesis. Furthermore, supplementation with bFGF changed the cytokine profiles of the cells and reduced their apoptotic level. Conclusion: Our study showed that bFGF supplementation of UC-MSC culture enhanced the cells' growth kinetics without compromising their nature.
format Article
author Ramasamy, Rajesh
Tong, C. K.
Yip, W. K.
Vellasamy, S.
Tan, B. C.
Seow, Heng Fong
spellingShingle Ramasamy, Rajesh
Tong, C. K.
Yip, W. K.
Vellasamy, S.
Tan, B. C.
Seow, Heng Fong
Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells.
author_facet Ramasamy, Rajesh
Tong, C. K.
Yip, W. K.
Vellasamy, S.
Tan, B. C.
Seow, Heng Fong
author_sort Ramasamy, Rajesh
title Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells.
title_short Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells.
title_full Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells.
title_fullStr Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells.
title_full_unstemmed Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells.
title_sort basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells.
publisher Wiley-Blackwell
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/24714/1/Basic%20fibroblast%20growth%20factor%20modulates%20cell%20cycle%20of%20human%20umbilical%20cord.pdf
_version_ 1819294786802155520
score 13.4562235

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