Staff View: Investigation of mortality in Macrobrachium rosenbergii larvae associated with hatchery management at Universiti Putra Malaysia Marine Science Research Centre, Port Dickson, Malaysia

Investigation of mortality in Macrobrachium rosenbergii larvae associated with hatchery management at Universiti Putra Malaysia Marine Science Research Centre, Port Dickson, Malaysia

Since the middle of year 2011, high mortalities in Macrobrachium rosenbergii larvae at Universiti Putra Malaysia Marine Science Research Centre, Port Dickson, Malaysia were regularly observed, which had caused severe economic losses. This research was carried out to investigate the biological and no...

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Main Authors:	Roslim, Noorashimah, Mohd Daud, Hassan
Format:	Conference or Workshop Item
Language:	English
Published:	2012
Online Access:	http://psasir.upm.edu.my/id/eprint/26826/1/PROCEEDING%2045.pdf
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spelling	oai:psasir.upm.edu.my:26826 http://psasir.upm.edu.my/id/eprint/26826/ Investigation of mortality in Macrobrachium rosenbergii larvae associated with hatchery management at Universiti Putra Malaysia Marine Science Research Centre, Port Dickson, Malaysia Roslim, Noorashimah Mohd Daud, Hassan Since the middle of year 2011, high mortalities in Macrobrachium rosenbergii larvae at Universiti Putra Malaysia Marine Science Research Centre, Port Dickson, Malaysia were regularly observed, which had caused severe economic losses. This research was carried out to investigate the biological and non-biological factors in the hatchery that contributed to disease development in order to postulate the relationship of the factors involved in larvae mortality and to examine the appropriate culture management approach to reduce mortality. Six experimental trials were carried out, namely physical examination of water quality parameters in the hatchery, microbiological studies of culture water, larvae, feed (including Artemia nauplii, egg custard, and blood cockles), and parasitological and mycological studies of culture water and larvae in hatcheries. The samples were taken twice a week. Physical quality of water was checked twice a day, in morning and evening using portable hand-held water quality kit. For bacteriology study, the isolation of bacteria were done using TSA and TCBS agar and identification using commercial test kits, BD BBL Crystal™ and API 20E™. Methylene blue stain was used for routine parasitology and mycology studies. The water quality in all tanks were within the recommended range, where the temperature was between 28-31°C for larvae and 27-32°C for brood stock. The pH for brood stock culture was between 7–7.2 while for the larvae it was between 7.2–7.4. The salinity ranged between 12–16 ppt for larvae and 3–5 ppt for brood stock. Two main bacteria were isolated during this study, which were Aeromonas hydrophila type II and Bukholderia cepaciae. A. hydrophila type II, B. cepaciae and Enterobacter spp. were normal flora of water and also opportunistic bacteria but could cause septicaemic diseases. No parasite or fungus was detected. In conclusion, early mortality in M. rosenbergii prawn larvae was closely related to hatchery management i.e. the use of green water system, feed preparation and sanitary procedures. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/26826/1/PROCEEDING%2045.pdf Roslim, Noorashimah and Mohd Daud, Hassan (2012) Investigation of mortality in Macrobrachium rosenbergii larvae associated with hatchery management at Universiti Putra Malaysia Marine Science Research Centre, Port Dickson, Malaysia. In: 7th Seminar in Veterinary Sciences, 27 Feb.-2 Mar. 2012, Faculty of Veterinary Medicine, Universiti Putra Malaysia. .
institution	UPM IR
collection	UPM IR
language	English
description	Since the middle of year 2011, high mortalities in Macrobrachium rosenbergii larvae at Universiti Putra Malaysia Marine Science Research Centre, Port Dickson, Malaysia were regularly observed, which had caused severe economic losses. This research was carried out to investigate the biological and non-biological factors in the hatchery that contributed to disease development in order to postulate the relationship of the factors involved in larvae mortality and to examine the appropriate culture management approach to reduce mortality. Six experimental trials were carried out, namely physical examination of water quality parameters in the hatchery, microbiological studies of culture water, larvae, feed (including Artemia nauplii, egg custard, and blood cockles), and parasitological and mycological studies of culture water and larvae in hatcheries. The samples were taken twice a week. Physical quality of water was checked twice a day, in morning and evening using portable hand-held water quality kit. For bacteriology study, the isolation of bacteria were done using TSA and TCBS agar and identification using commercial test kits, BD BBL Crystal™ and API 20E™. Methylene blue stain was used for routine parasitology and mycology studies. The water quality in all tanks were within the recommended range, where the temperature was between 28-31°C for larvae and 27-32°C for brood stock. The pH for brood stock culture was between 7–7.2 while for the larvae it was between 7.2–7.4. The salinity ranged between 12–16 ppt for larvae and 3–5 ppt for brood stock. Two main bacteria were isolated during this study, which were Aeromonas hydrophila type II and Bukholderia cepaciae. A. hydrophila type II, B. cepaciae and Enterobacter spp. were normal flora of water and also opportunistic bacteria but could cause septicaemic diseases. No parasite or fungus was detected. In conclusion, early mortality in M. rosenbergii prawn larvae was closely related to hatchery management i.e. the use of green water system, feed preparation and sanitary procedures.
format	Conference or Workshop Item
author	Roslim, Noorashimah Mohd Daud, Hassan
spellingShingle	Roslim, Noorashimah Mohd Daud, Hassan Investigation of mortality in Macrobrachium rosenbergii larvae associated with hatchery management at Universiti Putra Malaysia Marine Science Research Centre, Port Dickson, Malaysia
author_facet	Roslim, Noorashimah Mohd Daud, Hassan
author_sort	Roslim, Noorashimah
title	Investigation of mortality in Macrobrachium rosenbergii larvae associated with hatchery management at Universiti Putra Malaysia Marine Science Research Centre, Port Dickson, Malaysia
title_short	Investigation of mortality in Macrobrachium rosenbergii larvae associated with hatchery management at Universiti Putra Malaysia Marine Science Research Centre, Port Dickson, Malaysia
title_full	Investigation of mortality in Macrobrachium rosenbergii larvae associated with hatchery management at Universiti Putra Malaysia Marine Science Research Centre, Port Dickson, Malaysia
title_fullStr	Investigation of mortality in Macrobrachium rosenbergii larvae associated with hatchery management at Universiti Putra Malaysia Marine Science Research Centre, Port Dickson, Malaysia
title_full_unstemmed	Investigation of mortality in Macrobrachium rosenbergii larvae associated with hatchery management at Universiti Putra Malaysia Marine Science Research Centre, Port Dickson, Malaysia
title_sort	investigation of mortality in macrobrachium rosenbergii larvae associated with hatchery management at universiti putra malaysia marine science research centre, port dickson, malaysia
publishDate	2012
url	http://psasir.upm.edu.my/id/eprint/26826/1/PROCEEDING%2045.pdf
_version_	1819294979465412608
score	13.4562235

Investigation of mortality in Macrobrachium rosenbergii larvae associated with hatchery management at Universiti Putra Malaysia Marine Science Research Centre, Port Dickson, Malaysia

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