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Reproductive pathological changes in mice associated with Brucella melitensis and its lipopolysaccharides

Brucella melitensis is the gram-negative, aerobic bacteria that cause brucellosis in goats. Brucellosis causes abortion in wild and domestic animals resulting in enormous financial losses. There is still lack of knowledge of host cell response towards the immunogen of Brucella melitensis and the res...

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Asıl Yazarlar: Nik, Norasiah, Saharee, Abdul Aziz, Abdullah, Faez Firdaus Jesse
Materyal Türü: Conference or Workshop Item
Dil:English
Baskı/Yayın Bilgisi: 2012
Online Erişim:http://psasir.upm.edu.my/id/eprint/26834/1/PROCEEDING%2048.pdf
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spelling oai:psasir.upm.edu.my:26834 http://psasir.upm.edu.my/id/eprint/26834/ Reproductive pathological changes in mice associated with Brucella melitensis and its lipopolysaccharides Nik, Norasiah Saharee, Abdul Aziz Abdullah, Faez Firdaus Jesse Brucella melitensis is the gram-negative, aerobic bacteria that cause brucellosis in goats. Brucellosis causes abortion in wild and domestic animals resulting in enormous financial losses. There is still lack of knowledge of host cell response towards the immunogen of Brucella melitensis and the response of mice towards the LPS immunogen. In this experiment 3 groups of male mice were used and each group was inoculated with 1 mL PBS (pH 7), 1 × 109 Brucella melitensis and 1 mL lipopolysaccharides from 109 colonies of Brucella melitensis intraperitoneally to examine the clinical signs like mobility, eye discharge, closed eyes, ruffled hair and responsiveness exhibited in the mice. All 3 groups of mice that were inoculated with immunogens were observed for histopathological changes in the male reproductive organs like testes, vas deferens and seminal vesicle. The duration of observation was 5 days. After the fifth day all the mice that were still alive were euthanised by cervical dislocation and the histopathology lesions examined. Mice that were inoculated with the Brucella melitensis showed severe clinical signs. Mice infected with Brucella melitensis started to die from sixth hour post-inoculation. For the LPS group, the mice showed less movement beginning at 2 hours post-inoculation but became normal again after 5 hours post-inoculation. The control group exhibited normal clinical signs. Histopathology results showed mice inoculated with Brucella melitensis had moderate to severe atrophy of the spermatocytes of the testes and degenerative necrosis of the pseudostratified epithelium of vas deferens, while mice that were inoculated with LPS showed mild to moderate atrophy of the spermatocytes of the testes and moderate to severe degenerative necrosis of the pseudostratified epithelium of vas deferens. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/26834/1/PROCEEDING%2048.pdf Nik, Norasiah and Saharee, Abdul Aziz and Abdullah, Faez Firdaus Jesse (2012) Reproductive pathological changes in mice associated with Brucella melitensis and its lipopolysaccharides. In: 7th Seminar in Veterinary Sciences, 27 Feb.-2 Mar. 2012, Faculty of Veterinary Medicine, Universiti Putra Malaysia. .
institution UPM IR
collection UPM IR
language English
description Brucella melitensis is the gram-negative, aerobic bacteria that cause brucellosis in goats. Brucellosis causes abortion in wild and domestic animals resulting in enormous financial losses. There is still lack of knowledge of host cell response towards the immunogen of Brucella melitensis and the response of mice towards the LPS immunogen. In this experiment 3 groups of male mice were used and each group was inoculated with 1 mL PBS (pH 7), 1 × 109 Brucella melitensis and 1 mL lipopolysaccharides from 109 colonies of Brucella melitensis intraperitoneally to examine the clinical signs like mobility, eye discharge, closed eyes, ruffled hair and responsiveness exhibited in the mice. All 3 groups of mice that were inoculated with immunogens were observed for histopathological changes in the male reproductive organs like testes, vas deferens and seminal vesicle. The duration of observation was 5 days. After the fifth day all the mice that were still alive were euthanised by cervical dislocation and the histopathology lesions examined. Mice that were inoculated with the Brucella melitensis showed severe clinical signs. Mice infected with Brucella melitensis started to die from sixth hour post-inoculation. For the LPS group, the mice showed less movement beginning at 2 hours post-inoculation but became normal again after 5 hours post-inoculation. The control group exhibited normal clinical signs. Histopathology results showed mice inoculated with Brucella melitensis had moderate to severe atrophy of the spermatocytes of the testes and degenerative necrosis of the pseudostratified epithelium of vas deferens, while mice that were inoculated with LPS showed mild to moderate atrophy of the spermatocytes of the testes and moderate to severe degenerative necrosis of the pseudostratified epithelium of vas deferens.
format Conference or Workshop Item
author Nik, Norasiah
Saharee, Abdul Aziz
Abdullah, Faez Firdaus Jesse
spellingShingle Nik, Norasiah
Saharee, Abdul Aziz
Abdullah, Faez Firdaus Jesse
Reproductive pathological changes in mice associated with Brucella melitensis and its lipopolysaccharides
author_facet Nik, Norasiah
Saharee, Abdul Aziz
Abdullah, Faez Firdaus Jesse
author_sort Nik, Norasiah
title Reproductive pathological changes in mice associated with Brucella melitensis and its lipopolysaccharides
title_short Reproductive pathological changes in mice associated with Brucella melitensis and its lipopolysaccharides
title_full Reproductive pathological changes in mice associated with Brucella melitensis and its lipopolysaccharides
title_fullStr Reproductive pathological changes in mice associated with Brucella melitensis and its lipopolysaccharides
title_full_unstemmed Reproductive pathological changes in mice associated with Brucella melitensis and its lipopolysaccharides
title_sort reproductive pathological changes in mice associated with brucella melitensis and its lipopolysaccharides
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/26834/1/PROCEEDING%2048.pdf
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score 13.4562235

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