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Characterisation of inhibitory activity of metabolites produced by six strains of Lactobacillus plantarum.

Antibiotics have been used as therapeutic agents and growth promoters in animal feeds since 1950, However, the excessive use of antibiotics has led to resistance in bacterial pathogens and re-occurrence of eradicated diseases, This situation has urged for the search of an alternative to antibiotics...

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Główni autorzy: Foo, Hooi Ling, Karunakaramoorthy, Anuradha, Loh, Teck Chwen, Abdul Rahim, Raha, Nor Shamsudin, Mariana, Bejo, Mohd Hair
Format: Conference or Workshop Item
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English
Dostęp online:http://psasir.upm.edu.my/id/eprint/27250/1/ID%2027250.pdf
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spelling oai:psasir.upm.edu.my:27250 http://psasir.upm.edu.my/id/eprint/27250/ Characterisation of inhibitory activity of metabolites produced by six strains of Lactobacillus plantarum. Foo, Hooi Ling Karunakaramoorthy, Anuradha Loh, Teck Chwen Abdul Rahim, Raha Nor Shamsudin, Mariana Bejo, Mohd Hair Antibiotics have been used as therapeutic agents and growth promoters in animal feeds since 1950, However, the excessive use of antibiotics has led to resistance in bacterial pathogens and re-occurrence of eradicated diseases, This situation has urged for the search of an alternative to antibiotics in livestock production, LAB as probiotic is known to produce a wide variety of antibacterial substances; as well as inhibitory metabolites, Therefore, the general objective of this study is to characterize metabolite produced by six strains of locally isolated Lactobacillus plantarum, The results of stability test showed that metabolites were able to tolerate a wide range of pH, stable at moderate temperature but able to retain activity at different storage temperatures. Inhibitory test was conducted by agar well diffusion assay on selected aquaculture pathogens for 63 combinations of metabolites subsequently (1; UL4, 2; TL1, 3; RI11,4; RG14, 5; RS5 and 6; RG11). Based on total scores, the best combinations were 12456, 123456, 12356 and 23456, The versatile characteristics of the studied metabolites reveal the potential of their application in livestock, aquaculture and food industries respectively. Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/27250/1/ID%2027250.pdf Foo, Hooi Ling and Karunakaramoorthy, Anuradha and Loh, Teck Chwen and Abdul Rahim, Raha and Nor Shamsudin, Mariana and Bejo, Mohd Hair Characterisation of inhibitory activity of metabolites produced by six strains of Lactobacillus plantarum. In: The 7th International Symposium on LAB & Health, 29-31 May 2012, Woci, China. . English
institution UPM IR
collection UPM IR
language English
English
description Antibiotics have been used as therapeutic agents and growth promoters in animal feeds since 1950, However, the excessive use of antibiotics has led to resistance in bacterial pathogens and re-occurrence of eradicated diseases, This situation has urged for the search of an alternative to antibiotics in livestock production, LAB as probiotic is known to produce a wide variety of antibacterial substances; as well as inhibitory metabolites, Therefore, the general objective of this study is to characterize metabolite produced by six strains of locally isolated Lactobacillus plantarum, The results of stability test showed that metabolites were able to tolerate a wide range of pH, stable at moderate temperature but able to retain activity at different storage temperatures. Inhibitory test was conducted by agar well diffusion assay on selected aquaculture pathogens for 63 combinations of metabolites subsequently (1; UL4, 2; TL1, 3; RI11,4; RG14, 5; RS5 and 6; RG11). Based on total scores, the best combinations were 12456, 123456, 12356 and 23456, The versatile characteristics of the studied metabolites reveal the potential of their application in livestock, aquaculture and food industries respectively.
format Conference or Workshop Item
author Foo, Hooi Ling
Karunakaramoorthy, Anuradha
Loh, Teck Chwen
Abdul Rahim, Raha
Nor Shamsudin, Mariana
Bejo, Mohd Hair
spellingShingle Foo, Hooi Ling
Karunakaramoorthy, Anuradha
Loh, Teck Chwen
Abdul Rahim, Raha
Nor Shamsudin, Mariana
Bejo, Mohd Hair
Characterisation of inhibitory activity of metabolites produced by six strains of Lactobacillus plantarum.
author_facet Foo, Hooi Ling
Karunakaramoorthy, Anuradha
Loh, Teck Chwen
Abdul Rahim, Raha
Nor Shamsudin, Mariana
Bejo, Mohd Hair
author_sort Foo, Hooi Ling
title Characterisation of inhibitory activity of metabolites produced by six strains of Lactobacillus plantarum.
title_short Characterisation of inhibitory activity of metabolites produced by six strains of Lactobacillus plantarum.
title_full Characterisation of inhibitory activity of metabolites produced by six strains of Lactobacillus plantarum.
title_fullStr Characterisation of inhibitory activity of metabolites produced by six strains of Lactobacillus plantarum.
title_full_unstemmed Characterisation of inhibitory activity of metabolites produced by six strains of Lactobacillus plantarum.
title_sort characterisation of inhibitory activity of metabolites produced by six strains of lactobacillus plantarum.
url http://psasir.upm.edu.my/id/eprint/27250/1/ID%2027250.pdf
_version_ 1819295018830004224
score 13.4562235

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