Omslagsbillede

The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling

Viral-mediated oncolysis is a promising cancer therapeutic approach offering an increased efficacy with less toxicity than the current therapies. The complexity of solid tumor microenvironments includes regions of hypoxia. In these regions, the transcription factor, hypoxia inducible factor (HIF), i...

Fuld beskrivelse

Saved in:
Bibliografiske detaljer
Main Authors: Ch'ng, Wei Choong, Stanbridge, Eric J., Yusoff, Khatijah, Shafee, Norazizah
Format: Article
Sprog:English
Udgivet: Mary Ann Liebert 2013
Online adgang:http://psasir.upm.edu.my/id/eprint/28045/1/28045.pdf
Tags: Tilføj Tag
Ingen Tags, Vær først til at tagge denne postø!
id oai:psasir.upm.edu.my:28045
record_format eprints
spelling oai:psasir.upm.edu.my:28045 http://psasir.upm.edu.my/id/eprint/28045/ The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling Ch'ng, Wei Choong Stanbridge, Eric J. Yusoff, Khatijah Shafee, Norazizah Viral-mediated oncolysis is a promising cancer therapeutic approach offering an increased efficacy with less toxicity than the current therapies. The complexity of solid tumor microenvironments includes regions of hypoxia. In these regions, the transcription factor, hypoxia inducible factor (HIF), is active and regulates expression of many genes that contribute to aggressive malignancy, radio-, and chemo-resistance. To investigate the oncolytic efficacy of a highly virulent (velogenic) Newcastle disease virus (NDV) in the presence or absence of HIF-2α, renal cell carcinoma (RCC) cell lines with defective or reconstituted wild-type (wt) von Hippel-Lindau (VHL) activity were used. We show that these RCC cells responded to NDV by producing only interferon (IFN)-β, but not IFN-α, and are associated with increased STAT1 phosphorylation. Restoration of wt VHL expression enhanced NDV-induced IFN-β production, leading to prolonged STAT1 phosphorylation and increased cell death. Hypoxia augmented NDV oncolytic activity regardless of the cells' HIF-2α levels. These results highlight the potential of oncolytic NDV as a potent therapeutic agent in the killing of hypoxic cancer cells. Mary Ann Liebert 2013-07 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28045/1/28045.pdf Ch'ng, Wei Choong and Stanbridge, Eric J. and Yusoff, Khatijah and Shafee, Norazizah (2013) The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling. Journal of Interferon & Cytokine Research, 33 (7). pp. 346-354. ISSN 1079-9907; ESSN: 1557-7465 http://online.liebertpub.com/doi/abs/10.1089/jir.2012.0095 10.1089/jir.2012.0095
institution UPM IR
collection UPM IR
language English
description Viral-mediated oncolysis is a promising cancer therapeutic approach offering an increased efficacy with less toxicity than the current therapies. The complexity of solid tumor microenvironments includes regions of hypoxia. In these regions, the transcription factor, hypoxia inducible factor (HIF), is active and regulates expression of many genes that contribute to aggressive malignancy, radio-, and chemo-resistance. To investigate the oncolytic efficacy of a highly virulent (velogenic) Newcastle disease virus (NDV) in the presence or absence of HIF-2α, renal cell carcinoma (RCC) cell lines with defective or reconstituted wild-type (wt) von Hippel-Lindau (VHL) activity were used. We show that these RCC cells responded to NDV by producing only interferon (IFN)-β, but not IFN-α, and are associated with increased STAT1 phosphorylation. Restoration of wt VHL expression enhanced NDV-induced IFN-β production, leading to prolonged STAT1 phosphorylation and increased cell death. Hypoxia augmented NDV oncolytic activity regardless of the cells' HIF-2α levels. These results highlight the potential of oncolytic NDV as a potent therapeutic agent in the killing of hypoxic cancer cells.
format Article
author Ch'ng, Wei Choong
Stanbridge, Eric J.
Yusoff, Khatijah
Shafee, Norazizah
spellingShingle Ch'ng, Wei Choong
Stanbridge, Eric J.
Yusoff, Khatijah
Shafee, Norazizah
The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling
author_facet Ch'ng, Wei Choong
Stanbridge, Eric J.
Yusoff, Khatijah
Shafee, Norazizah
author_sort Ch'ng, Wei Choong
title The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling
title_short The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling
title_full The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling
title_fullStr The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling
title_full_unstemmed The oncolytic activity of Newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von Hippel-Lindau and interferon-β signaling
title_sort oncolytic activity of newcastle disease virus in clear cell renal carcinoma cells in normoxic and hypoxic conditions: the interplay between von hippel-lindau and interferon-β signaling
publisher Mary Ann Liebert
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/28045/1/28045.pdf
_version_ 1819295091989151744
score 13.4562235

Lignende værker