Cover Image

Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878)

Acetylcholinesterase (AChE) is generally used as an inhibitive assay for insecticides. A lesser-known property of AChE is its inhibition by heavy metals. In this work, we evaluate an AChE from silver catfish brain (Pangasius sp.), wasted from aquaculture industry, as an inhibitive assay for heavy me...

Full description

Saved in:
Bibliographic Details
Main Authors: Aidil, Mohd S., Sabullah, Mohd Khalidan, Halmi, Mohd Izuan Effendi, Sulaiman, Rosni, Shukor, Mohd Shukri, Abd. Shukor, Mohd. Yunus, Shaharuddin, Noor Azmi, Syed, Mohd Arif, Amir Hamzah, Amir Syahir
Format: Article
Language:English
Published: Parlar Scientific Publications 2013
Online Access:http://psasir.upm.edu.my/id/eprint/28046/1/280461.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:psasir.upm.edu.my:28046
record_format eprints
spelling oai:psasir.upm.edu.my:28046 http://psasir.upm.edu.my/id/eprint/28046/ Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878) Aidil, Mohd S. Sabullah, Mohd Khalidan Halmi, Mohd Izuan Effendi Sulaiman, Rosni Shukor, Mohd Shukri Abd. Shukor, Mohd. Yunus Shaharuddin, Noor Azmi Syed, Mohd Arif Amir Hamzah, Amir Syahir Acetylcholinesterase (AChE) is generally used as an inhibitive assay for insecticides. A lesser-known property of AChE is its inhibition by heavy metals. In this work, we evaluate an AChE from silver catfish brain (Pangasius sp.), wasted from aquaculture industry, as an inhibitive assay for heavy metals. We discovered that the AChE was completely inhibited by Cr6+, Ag 2+, Cd2+, Hg2+, Cu2+, Zn 2+ and Pb2+ during an initial screening. When testing at various concentrations, the above heavy metals exhibited exponential decay type inhibition curves. The calculated IC50 for Hg2+, Cu 2+, Ag , Cr6+, Cd2+, Pb2+, and Zn2+were 0.071, 0.088, 0.088, 0.87, 0.913, 1.1, and 1.572 mg/L, respectively. The Limit of Detection LOD and Limit of Quantification LOQ values of the heavy metals are within the range of the Maximum Permissibility Limit MPL allowed by the Malaysian Department of Environment DOE for Class-Ill rivers. The IC50 values for these heavy metals are comparable, and some are lower than those of the currently known assays, such as immobilised urease, Daphnia magna, Microtox™, rainbow trout, papain and bromelain assays. Field trial works on an industrial drainage site had shown that the developed assay is applicable in detecting heavy metals in the water. The assay can be carried out in less than 30 min under ambient temperature. Parlar Scientific Publications 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28046/1/280461.pdf Aidil, Mohd S. and Sabullah, Mohd Khalidan and Halmi, Mohd Izuan Effendi and Sulaiman, Rosni and Shukor, Mohd Shukri and Abd. Shukor, Mohd. Yunus and Shaharuddin, Noor Azmi and Syed, Mohd Arif and Amir Hamzah, Amir Syahir (2013) Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878). Fresenius Environmental Bulletin, 22 (12). pp. 3572-3576. ISSN 1018-4619
institution UPM IR
collection UPM IR
language English
description Acetylcholinesterase (AChE) is generally used as an inhibitive assay for insecticides. A lesser-known property of AChE is its inhibition by heavy metals. In this work, we evaluate an AChE from silver catfish brain (Pangasius sp.), wasted from aquaculture industry, as an inhibitive assay for heavy metals. We discovered that the AChE was completely inhibited by Cr6+, Ag 2+, Cd2+, Hg2+, Cu2+, Zn 2+ and Pb2+ during an initial screening. When testing at various concentrations, the above heavy metals exhibited exponential decay type inhibition curves. The calculated IC50 for Hg2+, Cu 2+, Ag , Cr6+, Cd2+, Pb2+, and Zn2+were 0.071, 0.088, 0.088, 0.87, 0.913, 1.1, and 1.572 mg/L, respectively. The Limit of Detection LOD and Limit of Quantification LOQ values of the heavy metals are within the range of the Maximum Permissibility Limit MPL allowed by the Malaysian Department of Environment DOE for Class-Ill rivers. The IC50 values for these heavy metals are comparable, and some are lower than those of the currently known assays, such as immobilised urease, Daphnia magna, Microtox™, rainbow trout, papain and bromelain assays. Field trial works on an industrial drainage site had shown that the developed assay is applicable in detecting heavy metals in the water. The assay can be carried out in less than 30 min under ambient temperature.
format Article
author Aidil, Mohd S.
Sabullah, Mohd Khalidan
Halmi, Mohd Izuan Effendi
Sulaiman, Rosni
Shukor, Mohd Shukri
Abd. Shukor, Mohd. Yunus
Shaharuddin, Noor Azmi
Syed, Mohd Arif
Amir Hamzah, Amir Syahir
spellingShingle Aidil, Mohd S.
Sabullah, Mohd Khalidan
Halmi, Mohd Izuan Effendi
Sulaiman, Rosni
Shukor, Mohd Shukri
Abd. Shukor, Mohd. Yunus
Shaharuddin, Noor Azmi
Syed, Mohd Arif
Amir Hamzah, Amir Syahir
Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878)
author_facet Aidil, Mohd S.
Sabullah, Mohd Khalidan
Halmi, Mohd Izuan Effendi
Sulaiman, Rosni
Shukor, Mohd Shukri
Abd. Shukor, Mohd. Yunus
Shaharuddin, Noor Azmi
Syed, Mohd Arif
Amir Hamzah, Amir Syahir
author_sort Aidil, Mohd S.
title Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878)
title_short Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878)
title_full Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878)
title_fullStr Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878)
title_full_unstemmed Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878)
title_sort assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from pangasius hypophthalmus (sauvage, 1878)
publisher Parlar Scientific Publications
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/28046/1/280461.pdf
_version_ 1819295092085620736
score 13.4562235

Similar Items