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Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source

This study presents the effect of carbon to nitrogen ratio (C/N) (mol/mol) on the cell growth and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation by Comamonas sp. EB172 in 2 L fermenters using volatile fatty acids (VFA) as the carbon source. This VFA was supplemented with ammonium sulphate...

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Main Authors: Zakaria @ Mamat, Mohd Rafein, Ariffin, Hidayah, Abd. Aziz, Suraini, Hassan, Mohd Ali, Shirai, Yoshihito
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2013
Online Access:http://psasir.upm.edu.my/id/eprint/28068/1/28068.pdf
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spelling oai:psasir.upm.edu.my:28068 http://psasir.upm.edu.my/id/eprint/28068/ Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source Zakaria @ Mamat, Mohd Rafein Ariffin, Hidayah Abd. Aziz, Suraini Hassan, Mohd Ali Shirai, Yoshihito This study presents the effect of carbon to nitrogen ratio (C/N) (mol/mol) on the cell growth and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation by Comamonas sp. EB172 in 2 L fermenters using volatile fatty acids (VFA) as the carbon source. This VFA was supplemented with ammonium sulphate and yeast extract in the feeding solution to achieve C/N (mol/mol) 5, 15, 25, and 34.4, respectively. By extrapolating the C/N and the source of nitrogen, the properties of the polymers can be regulated. The number average molecular weight (M n) of P(3HB-co-3HV) copolymer reached the highest at 838 × 10 3 Da with polydispersity index (PDI) value of 1.8, when the culture broth was supplemented with yeast extract (C/N 34.4). Tensile strength and Young's modulus of the copolymer containing 6-8 mol% 3HV were in the ranges of 13-14.4 MPa and 0.26-0.34 GPa, respectively, comparable to those of polyethylene (PE). Thus, Comamonas sp. EB172 has shown promising bacterial isolates producing polyhydroxyalkanoates from renewable carbon materials. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28068/1/28068.pdf Zakaria @ Mamat, Mohd Rafein and Ariffin, Hidayah and Abd. Aziz, Suraini and Hassan, Mohd Ali and Shirai, Yoshihito (2013) Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source. BioMed Research International, 2013. art. no. 237806. pp. 1-7. ISSN 2314-6133; ESSN: 2314-6141 http://www.hindawi.com/journals/bmri/2013/237806/abs/ 10.1155/2013/237806
institution UPM IR
collection UPM IR
language English
description This study presents the effect of carbon to nitrogen ratio (C/N) (mol/mol) on the cell growth and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation by Comamonas sp. EB172 in 2 L fermenters using volatile fatty acids (VFA) as the carbon source. This VFA was supplemented with ammonium sulphate and yeast extract in the feeding solution to achieve C/N (mol/mol) 5, 15, 25, and 34.4, respectively. By extrapolating the C/N and the source of nitrogen, the properties of the polymers can be regulated. The number average molecular weight (M n) of P(3HB-co-3HV) copolymer reached the highest at 838 × 10 3 Da with polydispersity index (PDI) value of 1.8, when the culture broth was supplemented with yeast extract (C/N 34.4). Tensile strength and Young's modulus of the copolymer containing 6-8 mol% 3HV were in the ranges of 13-14.4 MPa and 0.26-0.34 GPa, respectively, comparable to those of polyethylene (PE). Thus, Comamonas sp. EB172 has shown promising bacterial isolates producing polyhydroxyalkanoates from renewable carbon materials.
format Article
author Zakaria @ Mamat, Mohd Rafein
Ariffin, Hidayah
Abd. Aziz, Suraini
Hassan, Mohd Ali
Shirai, Yoshihito
spellingShingle Zakaria @ Mamat, Mohd Rafein
Ariffin, Hidayah
Abd. Aziz, Suraini
Hassan, Mohd Ali
Shirai, Yoshihito
Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source
author_facet Zakaria @ Mamat, Mohd Rafein
Ariffin, Hidayah
Abd. Aziz, Suraini
Hassan, Mohd Ali
Shirai, Yoshihito
author_sort Zakaria @ Mamat, Mohd Rafein
title Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source
title_short Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source
title_full Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source
title_fullStr Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source
title_full_unstemmed Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source
title_sort improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by comamonas sp. eb172 utilizing volatile fatty acids by regulating the nitrogen source
publisher Hindawi Publishing Corporation
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/28068/1/28068.pdf
_version_ 1819295094189064192
score 13.4562235

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