Antioxidant activity-guided separation of coumarins and lignan from Melicope glabra (Rutaceae)
The ethyl acetate and methanol bark extracts of Melicope glabra were evaluated for their antioxidant capacities by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and β-carotene bleaching/linoleic acid system. Both extracts exhibited strong inhibition against the DPPH radical (...
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2013
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oai:psasir.upm.edu.my:29557 http://psasir.upm.edu.my/id/eprint/29557/ Antioxidant activity-guided separation of coumarins and lignan from Melicope glabra (Rutaceae) Kassim, Nur Kartinee Rahmani, Mawardi Ismail, Amin Md.Sukari, Mohd. Aspollah Ee, Gwendoline Cheng Lian Md Nasir, Nadiah Awang, Khalijah The ethyl acetate and methanol bark extracts of Melicope glabra were evaluated for their antioxidant capacities by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and β-carotene bleaching/linoleic acid system. Both extracts exhibited strong inhibition against the DPPH radical (IC50 values of 24.81 and 13.01 μg ml(-1), respectively) and strong antioxidant activity in β-carotene bleaching assay. Both samples were found to have high phenolic content with values of 39 and 44 mg GAE/g as indicated by Follin-Ciocalteau's reagent. Antioxidant TLC assay-guided isolation on the methanol extract led to the isolation of a new pyranocoumarin, glabranin (1), umbelliferone (2), scopoletin (3) and sesamin (4), and their structures were determined by spectroscopy. Compounds (1-3) showed significant activities on DPPH free radical with the IC50 of 240.20, 810.02 and 413.19 μg ml(-1), respectively. However, in β-carotene bleaching assay, sesamin (4) showed higher inhibitory activity (1 mg ml(-1), 95%) than glabranin (1) (1 mg ml(-1), 74%), whilst umbelliferone (2) and scopoletin (3) were slightly pro-oxidant. Elsevier Science Limited 2013-08-15 Article PeerReviewed Kassim, Nur Kartinee and Rahmani, Mawardi and Ismail, Amin and Md.Sukari, Mohd. Aspollah and Ee, Gwendoline Cheng Lian and Md Nasir, Nadiah and Awang, Khalijah (2013) Antioxidant activity-guided separation of coumarins and lignan from Melicope glabra (Rutaceae). Food Chemistry, 139 (1-4). pp. 87-92. ISSN 0308-8146; ESSN: 1873-7072 http://www.sciencedirect.com/science/journal/03088146/139/1-4 10.1016/j.foodchem.2013.01.108 |
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The ethyl acetate and methanol bark extracts of Melicope glabra were evaluated for their antioxidant capacities by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and β-carotene bleaching/linoleic acid system. Both extracts exhibited strong inhibition against the DPPH radical (IC50 values of 24.81 and 13.01 μg ml(-1), respectively) and strong antioxidant activity in β-carotene bleaching assay. Both samples were found to have high phenolic content with values of 39 and 44 mg GAE/g as indicated by Follin-Ciocalteau's reagent. Antioxidant TLC assay-guided isolation on the methanol extract led to the isolation of a new pyranocoumarin, glabranin (1), umbelliferone (2), scopoletin (3) and sesamin (4), and their structures were determined by spectroscopy. Compounds (1-3) showed significant activities on DPPH free radical with the IC50 of 240.20, 810.02 and 413.19 μg ml(-1), respectively. However, in β-carotene bleaching assay, sesamin (4) showed higher inhibitory activity (1 mg ml(-1), 95%) than glabranin (1) (1 mg ml(-1), 74%), whilst umbelliferone (2) and scopoletin (3) were slightly pro-oxidant. |
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Article |
| author |
Kassim, Nur Kartinee Rahmani, Mawardi Ismail, Amin Md.Sukari, Mohd. Aspollah Ee, Gwendoline Cheng Lian Md Nasir, Nadiah Awang, Khalijah |
| spellingShingle |
Kassim, Nur Kartinee Rahmani, Mawardi Ismail, Amin Md.Sukari, Mohd. Aspollah Ee, Gwendoline Cheng Lian Md Nasir, Nadiah Awang, Khalijah Antioxidant activity-guided separation of coumarins and lignan from Melicope glabra (Rutaceae) |
| author_facet |
Kassim, Nur Kartinee Rahmani, Mawardi Ismail, Amin Md.Sukari, Mohd. Aspollah Ee, Gwendoline Cheng Lian Md Nasir, Nadiah Awang, Khalijah |
| author_sort |
Kassim, Nur Kartinee |
| title |
Antioxidant activity-guided separation of coumarins and lignan from Melicope glabra (Rutaceae) |
| title_short |
Antioxidant activity-guided separation of coumarins and lignan from Melicope glabra (Rutaceae) |
| title_full |
Antioxidant activity-guided separation of coumarins and lignan from Melicope glabra (Rutaceae) |
| title_fullStr |
Antioxidant activity-guided separation of coumarins and lignan from Melicope glabra (Rutaceae) |
| title_full_unstemmed |
Antioxidant activity-guided separation of coumarins and lignan from Melicope glabra (Rutaceae) |
| title_sort |
antioxidant activity-guided separation of coumarins and lignan from melicope glabra (rutaceae) |
| publisher |
Elsevier Science Limited |
| publishDate |
2013 |
| _version_ |
1819295238049497088 |
| score |
13.4562235 |