Imagen de Portada

5-Fluorouracil induce the expression of TLR4 on HCT116 colorectal cancer cell line expressing different variants of TLR4

Two common single nucleotide polymorphisms (SNPs) of the human TLR4 gene, namely Asp299Gly (D299G) and Thr399Ile (T399I), have been shown to impair the ability of certain individuals to respond properly to TLR4 ligands. 5-Fluorouracil (5-FU) is widely used for the treatment of patients with advanced...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores Principales: Davoodi, Homa, Hashemi, Seyed Reza, Seow, Heng Fong
Formato: Artículo
Lenguaje:English
Publicado: School of Pharmacy, Shaheed Beheshti Medical University 2013
Acceso en línea:http://psasir.upm.edu.my/id/eprint/29593/1/5.pdf
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:psasir.upm.edu.my:29593
record_format eprints
spelling oai:psasir.upm.edu.my:29593 http://psasir.upm.edu.my/id/eprint/29593/ 5-Fluorouracil induce the expression of TLR4 on HCT116 colorectal cancer cell line expressing different variants of TLR4 Davoodi, Homa Hashemi, Seyed Reza Seow, Heng Fong Two common single nucleotide polymorphisms (SNPs) of the human TLR4 gene, namely Asp299Gly (D299G) and Thr399Ile (T399I), have been shown to impair the ability of certain individuals to respond properly to TLR4 ligands. 5-Fluorouracil (5-FU) is widely used for the treatment of patients with advanced colon cancers. The present study examined the impact of two common polymorphisms of the TLR4 genes on the response of the HCT116 colorectal cancer cells to 5-FU. HCT116 was transfected with Flag-CMV1-TLR4 wild-type (WT) and D299G, T399I expression plasmids. The cytotoxic effect of 5-FU on transfected cells was assessed by MTT assay. FACS analysis was performed to show the effect of 5-FU and LPS on the expression of different variants of TLR4. The lowest IC50-value was measured in cells expressing the WT TLR4 and non-transfected cells were more resistance to the drug compared to the other cells. 5-FU significantly induced the expression of TLR4 protein in the presence and absence of LPS. 5-FU also induced HMGB1 secretion, Cas3 and PARP activity and these effects were stronger in cells expressing WT TLR4 than the other cells. In conclusion, 5-FU-induced TLR4 expression and LPS had synergistic effect with 5-FU to induced apoptosis in colorectal cancer cells. School of Pharmacy, Shaheed Beheshti Medical University 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29593/1/5.pdf Davoodi, Homa and Hashemi, Seyed Reza and Seow, Heng Fong (2013) 5-Fluorouracil induce the expression of TLR4 on HCT116 colorectal cancer cell line expressing different variants of TLR4. Iranian Journal of Pharmaceutical Research, 12 (2). pp. 453-460. ISSN 1735-0328; ESSN: 1726-6890 http://ijpr.sbmu.ac.ir/article_1308_55.html
institution UPM IR
collection UPM IR
language English
description Two common single nucleotide polymorphisms (SNPs) of the human TLR4 gene, namely Asp299Gly (D299G) and Thr399Ile (T399I), have been shown to impair the ability of certain individuals to respond properly to TLR4 ligands. 5-Fluorouracil (5-FU) is widely used for the treatment of patients with advanced colon cancers. The present study examined the impact of two common polymorphisms of the TLR4 genes on the response of the HCT116 colorectal cancer cells to 5-FU. HCT116 was transfected with Flag-CMV1-TLR4 wild-type (WT) and D299G, T399I expression plasmids. The cytotoxic effect of 5-FU on transfected cells was assessed by MTT assay. FACS analysis was performed to show the effect of 5-FU and LPS on the expression of different variants of TLR4. The lowest IC50-value was measured in cells expressing the WT TLR4 and non-transfected cells were more resistance to the drug compared to the other cells. 5-FU significantly induced the expression of TLR4 protein in the presence and absence of LPS. 5-FU also induced HMGB1 secretion, Cas3 and PARP activity and these effects were stronger in cells expressing WT TLR4 than the other cells. In conclusion, 5-FU-induced TLR4 expression and LPS had synergistic effect with 5-FU to induced apoptosis in colorectal cancer cells.
format Article
author Davoodi, Homa
Hashemi, Seyed Reza
Seow, Heng Fong
spellingShingle Davoodi, Homa
Hashemi, Seyed Reza
Seow, Heng Fong
5-Fluorouracil induce the expression of TLR4 on HCT116 colorectal cancer cell line expressing different variants of TLR4
author_facet Davoodi, Homa
Hashemi, Seyed Reza
Seow, Heng Fong
author_sort Davoodi, Homa
title 5-Fluorouracil induce the expression of TLR4 on HCT116 colorectal cancer cell line expressing different variants of TLR4
title_short 5-Fluorouracil induce the expression of TLR4 on HCT116 colorectal cancer cell line expressing different variants of TLR4
title_full 5-Fluorouracil induce the expression of TLR4 on HCT116 colorectal cancer cell line expressing different variants of TLR4
title_fullStr 5-Fluorouracil induce the expression of TLR4 on HCT116 colorectal cancer cell line expressing different variants of TLR4
title_full_unstemmed 5-Fluorouracil induce the expression of TLR4 on HCT116 colorectal cancer cell line expressing different variants of TLR4
title_sort 5-fluorouracil induce the expression of tlr4 on hct116 colorectal cancer cell line expressing different variants of tlr4
publisher School of Pharmacy, Shaheed Beheshti Medical University
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29593/1/5.pdf
_version_ 1819295241480437760
score 13.4562235

Ejemplares similares