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Transcriptional profiling of feline infectious peritonitis virus infection in CRFK cells and in PBMCs from FIP diagnosed cats

Feline Infectious Peritonitis (FIP) is a lethal systemic disease, caused by the FIP Virus (FIPV); a virulent mutant of Feline Enteric Coronavirus (FECV). Currently, the viruses virulence determinants and host gene expressions during FIPV infection are not fully understood. RNA sequencing of Crandel...

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Main Authors: Harun, Mohammad Syamsul Reza, Choong, Oi Kuan, Selvarajah, Gayathri Thevi, Tan, Sheau Wei, Arshad, Siti Suri, Bejo, Mohd Hair, Omar, Abdul Rahman
Format: Article
Language:English
Published: BioMed Central 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29885/1/Transcriptional%20profiling%20of%20feline%20infectious%20peritonitis%20virus%20infection%20in%20CRFK%20cells%20and%20in%20PBMCs%20from%20FIP%20diagnosed%20cats.pdf
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spelling oai:psasir.upm.edu.my:29885 http://psasir.upm.edu.my/id/eprint/29885/ Transcriptional profiling of feline infectious peritonitis virus infection in CRFK cells and in PBMCs from FIP diagnosed cats Harun, Mohammad Syamsul Reza Choong, Oi Kuan Selvarajah, Gayathri Thevi Tan, Sheau Wei Arshad, Siti Suri Bejo, Mohd Hair Omar, Abdul Rahman Feline Infectious Peritonitis (FIP) is a lethal systemic disease, caused by the FIP Virus (FIPV); a virulent mutant of Feline Enteric Coronavirus (FECV). Currently, the viruses virulence determinants and host gene expressions during FIPV infection are not fully understood. RNA sequencing of Crandell Rees Feline Kidney (CRFK) cells, infected with FIPV strain 79–1146 at 3 hours post infection (h.p.i), were sequenced using the Illumina next generation sequencing approach. Bioinformatic’s analysis, based on Felis catus 2X annotated shotgun reference genome, using CLC bio Genome Workbench mapped both control and infected cell reads to 18899 genes out of 19046 annotated genes. Kal’s Z test statistical analysis was used to analyse the differentially expressed genes from the infected CRFK cells. Real time RT-qPCR was developed for further transcriptional profiling of three genes (PD-1, PD-L1 and A3H) in infected CRFK cells and Peripheral Blood Mononuclear Cells (PBMCs) from healthy and FIP-diseased cats. BioMed Central 2013-11-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29885/1/Transcriptional%20profiling%20of%20feline%20infectious%20peritonitis%20virus%20infection%20in%20CRFK%20cells%20and%20in%20PBMCs%20from%20FIP%20diagnosed%20cats.pdf Harun, Mohammad Syamsul Reza and Choong, Oi Kuan and Selvarajah, Gayathri Thevi and Tan, Sheau Wei and Arshad, Siti Suri and Bejo, Mohd Hair and Omar, Abdul Rahman (2013) Transcriptional profiling of feline infectious peritonitis virus infection in CRFK cells and in PBMCs from FIP diagnosed cats. Virology Journal, 10. art. no. 329. pp. 1-9. ISSN 1743-422X 10.1186/1743-422X-10-329
institution UPM IR
collection UPM IR
language English
description Feline Infectious Peritonitis (FIP) is a lethal systemic disease, caused by the FIP Virus (FIPV); a virulent mutant of Feline Enteric Coronavirus (FECV). Currently, the viruses virulence determinants and host gene expressions during FIPV infection are not fully understood. RNA sequencing of Crandell Rees Feline Kidney (CRFK) cells, infected with FIPV strain 79–1146 at 3 hours post infection (h.p.i), were sequenced using the Illumina next generation sequencing approach. Bioinformatic’s analysis, based on Felis catus 2X annotated shotgun reference genome, using CLC bio Genome Workbench mapped both control and infected cell reads to 18899 genes out of 19046 annotated genes. Kal’s Z test statistical analysis was used to analyse the differentially expressed genes from the infected CRFK cells. Real time RT-qPCR was developed for further transcriptional profiling of three genes (PD-1, PD-L1 and A3H) in infected CRFK cells and Peripheral Blood Mononuclear Cells (PBMCs) from healthy and FIP-diseased cats.
format Article
author Harun, Mohammad Syamsul Reza
Choong, Oi Kuan
Selvarajah, Gayathri Thevi
Tan, Sheau Wei
Arshad, Siti Suri
Bejo, Mohd Hair
Omar, Abdul Rahman
spellingShingle Harun, Mohammad Syamsul Reza
Choong, Oi Kuan
Selvarajah, Gayathri Thevi
Tan, Sheau Wei
Arshad, Siti Suri
Bejo, Mohd Hair
Omar, Abdul Rahman
Transcriptional profiling of feline infectious peritonitis virus infection in CRFK cells and in PBMCs from FIP diagnosed cats
author_facet Harun, Mohammad Syamsul Reza
Choong, Oi Kuan
Selvarajah, Gayathri Thevi
Tan, Sheau Wei
Arshad, Siti Suri
Bejo, Mohd Hair
Omar, Abdul Rahman
author_sort Harun, Mohammad Syamsul Reza
title Transcriptional profiling of feline infectious peritonitis virus infection in CRFK cells and in PBMCs from FIP diagnosed cats
title_short Transcriptional profiling of feline infectious peritonitis virus infection in CRFK cells and in PBMCs from FIP diagnosed cats
title_full Transcriptional profiling of feline infectious peritonitis virus infection in CRFK cells and in PBMCs from FIP diagnosed cats
title_fullStr Transcriptional profiling of feline infectious peritonitis virus infection in CRFK cells and in PBMCs from FIP diagnosed cats
title_full_unstemmed Transcriptional profiling of feline infectious peritonitis virus infection in CRFK cells and in PBMCs from FIP diagnosed cats
title_sort transcriptional profiling of feline infectious peritonitis virus infection in crfk cells and in pbmcs from fip diagnosed cats
publisher BioMed Central
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29885/1/Transcriptional%20profiling%20of%20feline%20infectious%20peritonitis%20virus%20infection%20in%20CRFK%20cells%20and%20in%20PBMCs%20from%20FIP%20diagnosed%20cats.pdf
_version_ 1819295269340053504
score 13.4562235

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