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Clinical and reproductive pathological changes associated with Brucella melitensis and its lipopolysaccharides in female mice via oral inoculation

Brucella melitensis (B. melitensis) are Gram-negative, aerobic, facultative intracellular bacteria that cause brucellosis that usually leads to abortion in sheep and goats. Three groups of equal number of 24 healthy female mice were used as animal models. They were orally inoculated with 0.4 mL of p...

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Principais autores: Abdullah, Faez Firdaus Jesse, Adamu, Lawan, Adnan, Nur Hazirah, Osman, Abdinasir Yusuf, Mansor, Rozaihan, Haron, Abd Wahid, Saad, Mohd Zamri, Omar, Abdul Rahman, Saharee, Abdul Aziz
Formato: Atigo
Idioma:English
Publicado em: Science Publications 2013
Acesso em linha:http://psasir.upm.edu.my/id/eprint/29910/1/ajavsp.2013.104.111.pdf
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spelling oai:psasir.upm.edu.my:29910 http://psasir.upm.edu.my/id/eprint/29910/ Clinical and reproductive pathological changes associated with Brucella melitensis and its lipopolysaccharides in female mice via oral inoculation Abdullah, Faez Firdaus Jesse Adamu, Lawan Adnan, Nur Hazirah Osman, Abdinasir Yusuf Mansor, Rozaihan Haron, Abd Wahid Saad, Mohd Zamri Omar, Abdul Rahman Saharee, Abdul Aziz Brucella melitensis (B. melitensis) are Gram-negative, aerobic, facultative intracellular bacteria that cause brucellosis that usually leads to abortion in sheep and goats. Three groups of equal number of 24 healthy female mice were used as animal models. They were orally inoculated with 0.4 mL of phosphate Buffered Saline (PBS-Control group), 0.4 mL of 109 cfu of B. melitensis and 0.4 mL of Lipopolysaccharides (LPS) extracted from 109cfu of B. melitensis (both as treatment groups). Clinical signs exhibited by the mice were observed for 10 days, after which the survived mice were euthanized by cervical dislocation. Following that, post mortem was conducted and histopathological study of the reproductive organs was carried out. B. melitensis group showed mild clinical signs compared to LPS group which showed normal behaviours except for mild ruffled fur, 14 and 34 h post-inoculation, respectively. The control group (PBS) showed normal behaviours. Histopathology results revealed that both B. melitensis and LPS groups showed mild to moderate infiltration of inflammatory cells in the reproductive organs, along with normal to mild findings of necrosis. Mild to moderate haemorrhage were found in the mice of B. melitensis group, while LPS group showed normal to mild haemorrhage and moderate to severe congestion of the ovary. The study proved that mice infected orally with B. melitensis developed mild clinical signs whereas mice orally inoculated by its LPS showed normal behavior except for the mild ruffled fur. Moreover, both groups of mice inoculated with B. melitensis immunogens developed pathological changes in the reproductive organs. The LPS of B. melitensis could be a potential candidate for the development of vaccines. Science Publications 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29910/1/ajavsp.2013.104.111.pdf Abdullah, Faez Firdaus Jesse and Adamu, Lawan and Adnan, Nur Hazirah and Osman, Abdinasir Yusuf and Mansor, Rozaihan and Haron, Abd Wahid and Saad, Mohd Zamri and Omar, Abdul Rahman and Saharee, Abdul Aziz (2013) Clinical and reproductive pathological changes associated with Brucella melitensis and its lipopolysaccharides in female mice via oral inoculation. American Journal of Animal and Veterinary Sciences, 8 (3). pp. 104-111. ISSN 1557-4555; ESSN: 1557-4563 http://thescipub.com/abstract/10.3844/ajavsp.2013.104.111 10.3844/ajavsp.2013.104.111
institution UPM IR
collection UPM IR
language English
description Brucella melitensis (B. melitensis) are Gram-negative, aerobic, facultative intracellular bacteria that cause brucellosis that usually leads to abortion in sheep and goats. Three groups of equal number of 24 healthy female mice were used as animal models. They were orally inoculated with 0.4 mL of phosphate Buffered Saline (PBS-Control group), 0.4 mL of 109 cfu of B. melitensis and 0.4 mL of Lipopolysaccharides (LPS) extracted from 109cfu of B. melitensis (both as treatment groups). Clinical signs exhibited by the mice were observed for 10 days, after which the survived mice were euthanized by cervical dislocation. Following that, post mortem was conducted and histopathological study of the reproductive organs was carried out. B. melitensis group showed mild clinical signs compared to LPS group which showed normal behaviours except for mild ruffled fur, 14 and 34 h post-inoculation, respectively. The control group (PBS) showed normal behaviours. Histopathology results revealed that both B. melitensis and LPS groups showed mild to moderate infiltration of inflammatory cells in the reproductive organs, along with normal to mild findings of necrosis. Mild to moderate haemorrhage were found in the mice of B. melitensis group, while LPS group showed normal to mild haemorrhage and moderate to severe congestion of the ovary. The study proved that mice infected orally with B. melitensis developed mild clinical signs whereas mice orally inoculated by its LPS showed normal behavior except for the mild ruffled fur. Moreover, both groups of mice inoculated with B. melitensis immunogens developed pathological changes in the reproductive organs. The LPS of B. melitensis could be a potential candidate for the development of vaccines.
format Article
author Abdullah, Faez Firdaus Jesse
Adamu, Lawan
Adnan, Nur Hazirah
Osman, Abdinasir Yusuf
Mansor, Rozaihan
Haron, Abd Wahid
Saad, Mohd Zamri
Omar, Abdul Rahman
Saharee, Abdul Aziz
spellingShingle Abdullah, Faez Firdaus Jesse
Adamu, Lawan
Adnan, Nur Hazirah
Osman, Abdinasir Yusuf
Mansor, Rozaihan
Haron, Abd Wahid
Saad, Mohd Zamri
Omar, Abdul Rahman
Saharee, Abdul Aziz
Clinical and reproductive pathological changes associated with Brucella melitensis and its lipopolysaccharides in female mice via oral inoculation
author_facet Abdullah, Faez Firdaus Jesse
Adamu, Lawan
Adnan, Nur Hazirah
Osman, Abdinasir Yusuf
Mansor, Rozaihan
Haron, Abd Wahid
Saad, Mohd Zamri
Omar, Abdul Rahman
Saharee, Abdul Aziz
author_sort Abdullah, Faez Firdaus Jesse
title Clinical and reproductive pathological changes associated with Brucella melitensis and its lipopolysaccharides in female mice via oral inoculation
title_short Clinical and reproductive pathological changes associated with Brucella melitensis and its lipopolysaccharides in female mice via oral inoculation
title_full Clinical and reproductive pathological changes associated with Brucella melitensis and its lipopolysaccharides in female mice via oral inoculation
title_fullStr Clinical and reproductive pathological changes associated with Brucella melitensis and its lipopolysaccharides in female mice via oral inoculation
title_full_unstemmed Clinical and reproductive pathological changes associated with Brucella melitensis and its lipopolysaccharides in female mice via oral inoculation
title_sort clinical and reproductive pathological changes associated with brucella melitensis and its lipopolysaccharides in female mice via oral inoculation
publisher Science Publications
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29910/1/ajavsp.2013.104.111.pdf
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score 13.4562235

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