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Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia

Apart from occasional reports of clinical disease affecting horses, there is no information about Trypanosoma evansi in horses in Peninsula Malaysia. Thus, a cross-sectional study was conducted in eight states in Peninsula Malaysia to determine the active presence of T. evansi in horses. A total of...

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Main Authors: Elshafie, E. I., Abdullah Sani, Rehana, Hassan, Latiffah, Kumar Sharma, Reuben Sunil, Fateh Mohamed, Bashir Ahmad, Abubakar, I. A.
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Published: Malaysian Society of Parasitology and Tropical Medicine 2013
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spelling oai:psasir.upm.edu.my:29926 http://psasir.upm.edu.my/id/eprint/29926/ Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia Elshafie, E. I. Abdullah Sani, Rehana Hassan, Latiffah Kumar Sharma, Reuben Sunil Fateh Mohamed, Bashir Ahmad Abubakar, I. A. Apart from occasional reports of clinical disease affecting horses, there is no information about Trypanosoma evansi in horses in Peninsula Malaysia. Thus, a cross-sectional study was conducted in eight states in Peninsula Malaysia to determine the active presence of T. evansi in horses. A total of 527 blood samples were obtained and examined by haematocrit centrifugation technique (HCT), Giemsa-stained thin blood smear (GSS), morphometric measurements, polymerase chain reaction (PCR) and cloning of PCR products. The results showed an overall parasitological prevalence of 0.57% (3/527, CI: 1.6-0.19%) with both HCT and GSS. Morphometric study revealed the mean total length of the trypanosomes including the free flagellum was 27.94 ± 2.63 µm. PCR successfully amplified a trypanosome specific 257 bp in 1.14% of samples (6/527, CI: 2.4-0.52%) and was confirmed by nucleotide sequences. The mean packed cell volume (PCV) for the positive cases detected by HCT was lower (23% ± 7.00) compared to the positive cases detected by PCR alone in the state of Terengganu (35% ± 4.73). In conclusion, this study showed T. evansi infection occurred in low frequency in horses in Peninsula Malaysia, and anaemia coincided with parasitaemic animals. PCR is considered as a sensitive diagnostic tool when parasitaemia is undetectable. The slight lengthier mean of parasite and anaemia may indicate a virulent strain of T. evansi circulating throughout the country. Thus, it’s highly recommended to shed light on host-parasite relationship for better epidemiological understanding. Malaysian Society of Parasitology and Tropical Medicine 2013 Article PeerReviewed Elshafie, E. I. and Abdullah Sani, Rehana and Hassan, Latiffah and Kumar Sharma, Reuben Sunil and Fateh Mohamed, Bashir Ahmad and Abubakar, I. A. (2013) Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia. Tropical Biomedicine, 30 (3). pp. 444-450. ISSN 0127-5720 http://www.msptm.org/journal27.html
institution UPM IR
collection UPM IR
description Apart from occasional reports of clinical disease affecting horses, there is no information about Trypanosoma evansi in horses in Peninsula Malaysia. Thus, a cross-sectional study was conducted in eight states in Peninsula Malaysia to determine the active presence of T. evansi in horses. A total of 527 blood samples were obtained and examined by haematocrit centrifugation technique (HCT), Giemsa-stained thin blood smear (GSS), morphometric measurements, polymerase chain reaction (PCR) and cloning of PCR products. The results showed an overall parasitological prevalence of 0.57% (3/527, CI: 1.6-0.19%) with both HCT and GSS. Morphometric study revealed the mean total length of the trypanosomes including the free flagellum was 27.94 ± 2.63 µm. PCR successfully amplified a trypanosome specific 257 bp in 1.14% of samples (6/527, CI: 2.4-0.52%) and was confirmed by nucleotide sequences. The mean packed cell volume (PCV) for the positive cases detected by HCT was lower (23% ± 7.00) compared to the positive cases detected by PCR alone in the state of Terengganu (35% ± 4.73). In conclusion, this study showed T. evansi infection occurred in low frequency in horses in Peninsula Malaysia, and anaemia coincided with parasitaemic animals. PCR is considered as a sensitive diagnostic tool when parasitaemia is undetectable. The slight lengthier mean of parasite and anaemia may indicate a virulent strain of T. evansi circulating throughout the country. Thus, it’s highly recommended to shed light on host-parasite relationship for better epidemiological understanding.
format Article
author Elshafie, E. I.
Abdullah Sani, Rehana
Hassan, Latiffah
Kumar Sharma, Reuben Sunil
Fateh Mohamed, Bashir Ahmad
Abubakar, I. A.
spellingShingle Elshafie, E. I.
Abdullah Sani, Rehana
Hassan, Latiffah
Kumar Sharma, Reuben Sunil
Fateh Mohamed, Bashir Ahmad
Abubakar, I. A.
Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia
author_facet Elshafie, E. I.
Abdullah Sani, Rehana
Hassan, Latiffah
Kumar Sharma, Reuben Sunil
Fateh Mohamed, Bashir Ahmad
Abubakar, I. A.
author_sort Elshafie, E. I.
title Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia
title_short Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia
title_full Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia
title_fullStr Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia
title_full_unstemmed Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia
title_sort active infection and morphometric study of trypanosoma evansi among horses in peninsula malaysia
publisher Malaysian Society of Parasitology and Tropical Medicine
publishDate 2013
_version_ 1819295273220833280
score 13.4562235

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