Buchumschlag

Potency and efficacy of a low pathogenic H5N2 inactivated vaccine against challenge with a Malaysian H5N1 highly pathogenic avian influenza virus

The potency and efficacy of an avian influenza (AI) H5N2 inactivated vaccine that was developed at Veterinary Research Institute, Ipoh was tested. The percentage sequence identity of the HA gene of the H5N2 vaccine virus to the challenge virus [A/chicken/Malaysia/5858/04 (H5N1)] was 88.2% by nucleot...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Syed Hassan, Sharifah, M. Noor, Suriani, Mohamed, Maizan, Ong, G. H., D., Azizah, K., Suzana, Omar, Abdul Rahman, Ideris, Aini
Format: Artikel
Sprache:English
Veröffentlicht: Veterinary Association Malaysia 2013
Online Zugang:http://psasir.upm.edu.my/id/eprint/32858/1/9.%20Potency%20and%20efficacy%20of%20a%20low%20pathogenic%20H5N2%20inactivated%20vaccine%20against%20challenge%20with%20a%20Malaysian%20H5N1%20highly%20pathogenic%20avian%20influenza%20virus.pdf
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
id oai:psasir.upm.edu.my:32858
record_format eprints
spelling oai:psasir.upm.edu.my:32858 http://psasir.upm.edu.my/id/eprint/32858/ Potency and efficacy of a low pathogenic H5N2 inactivated vaccine against challenge with a Malaysian H5N1 highly pathogenic avian influenza virus Syed Hassan, Sharifah M. Noor, Suriani Mohamed, Maizan Ong, G. H. D., Azizah K., Suzana Omar, Abdul Rahman Ideris, Aini The potency and efficacy of an avian influenza (AI) H5N2 inactivated vaccine that was developed at Veterinary Research Institute, Ipoh was tested. The percentage sequence identity of the HA gene of the H5N2 vaccine virus to the challenge virus [A/chicken/Malaysia/5858/04 (H5N1)] was 88.2% by nucleotide and 90% by amino acid sequences similarities, respectively. As for the HAI segment, the nucleotide sequence similarities were 88.3 % and by amino acid sequence 87.7%.For potency testing, the heterologous killed H5N2 AI vaccine, formulated as an oil emulsion was administered only once subcutaneously in twenty five two-week old commercial broiler chickens. The HI antibodies were not detectable at week 1 post vaccination. The HI GMT attained was 30, 63, 200, 54 and 32 by week 2, 3, 4, 5, and 6 post vaccinations. Efficacy study was conducted on ten SPF chickens at week 3 post vaccination. 60% of the birds (6/10) with HI titres ≥ 64 - 128 survived the challenged. H5N1 challenge virus was reisolated from all the birds with HI titre ≤ 32 that died, and each of the birds that survived with HI titres of 64 and 128, from the oropharynx and cloaca at day 3 post challenge. This vaccine protected 60% of chickens against mortality and did not prevent shedding after challenged with a HPAI H5N1 virus. Veterinary Association Malaysia 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/32858/1/9.%20Potency%20and%20efficacy%20of%20a%20low%20pathogenic%20H5N2%20inactivated%20vaccine%20against%20challenge%20with%20a%20Malaysian%20H5N1%20highly%20pathogenic%20avian%20influenza%20virus.pdf Syed Hassan, Sharifah and M. Noor, Suriani and Mohamed, Maizan and Ong, G. H. and D., Azizah and K., Suzana and Omar, Abdul Rahman and Ideris, Aini (2013) Potency and efficacy of a low pathogenic H5N2 inactivated vaccine against challenge with a Malaysian H5N1 highly pathogenic avian influenza virus. Jurnal Veterinar Malaysia, 25 (1&2). pp. 1-6. ISSN 9128-2506
institution UPM IR
collection UPM IR
language English
description The potency and efficacy of an avian influenza (AI) H5N2 inactivated vaccine that was developed at Veterinary Research Institute, Ipoh was tested. The percentage sequence identity of the HA gene of the H5N2 vaccine virus to the challenge virus [A/chicken/Malaysia/5858/04 (H5N1)] was 88.2% by nucleotide and 90% by amino acid sequences similarities, respectively. As for the HAI segment, the nucleotide sequence similarities were 88.3 % and by amino acid sequence 87.7%.For potency testing, the heterologous killed H5N2 AI vaccine, formulated as an oil emulsion was administered only once subcutaneously in twenty five two-week old commercial broiler chickens. The HI antibodies were not detectable at week 1 post vaccination. The HI GMT attained was 30, 63, 200, 54 and 32 by week 2, 3, 4, 5, and 6 post vaccinations. Efficacy study was conducted on ten SPF chickens at week 3 post vaccination. 60% of the birds (6/10) with HI titres ≥ 64 - 128 survived the challenged. H5N1 challenge virus was reisolated from all the birds with HI titre ≤ 32 that died, and each of the birds that survived with HI titres of 64 and 128, from the oropharynx and cloaca at day 3 post challenge. This vaccine protected 60% of chickens against mortality and did not prevent shedding after challenged with a HPAI H5N1 virus.
format Article
author Syed Hassan, Sharifah
M. Noor, Suriani
Mohamed, Maizan
Ong, G. H.
D., Azizah
K., Suzana
Omar, Abdul Rahman
Ideris, Aini
spellingShingle Syed Hassan, Sharifah
M. Noor, Suriani
Mohamed, Maizan
Ong, G. H.
D., Azizah
K., Suzana
Omar, Abdul Rahman
Ideris, Aini
Potency and efficacy of a low pathogenic H5N2 inactivated vaccine against challenge with a Malaysian H5N1 highly pathogenic avian influenza virus
author_facet Syed Hassan, Sharifah
M. Noor, Suriani
Mohamed, Maizan
Ong, G. H.
D., Azizah
K., Suzana
Omar, Abdul Rahman
Ideris, Aini
author_sort Syed Hassan, Sharifah
title Potency and efficacy of a low pathogenic H5N2 inactivated vaccine against challenge with a Malaysian H5N1 highly pathogenic avian influenza virus
title_short Potency and efficacy of a low pathogenic H5N2 inactivated vaccine against challenge with a Malaysian H5N1 highly pathogenic avian influenza virus
title_full Potency and efficacy of a low pathogenic H5N2 inactivated vaccine against challenge with a Malaysian H5N1 highly pathogenic avian influenza virus
title_fullStr Potency and efficacy of a low pathogenic H5N2 inactivated vaccine against challenge with a Malaysian H5N1 highly pathogenic avian influenza virus
title_full_unstemmed Potency and efficacy of a low pathogenic H5N2 inactivated vaccine against challenge with a Malaysian H5N1 highly pathogenic avian influenza virus
title_sort potency and efficacy of a low pathogenic h5n2 inactivated vaccine against challenge with a malaysian h5n1 highly pathogenic avian influenza virus
publisher Veterinary Association Malaysia
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/32858/1/9.%20Potency%20and%20efficacy%20of%20a%20low%20pathogenic%20H5N2%20inactivated%20vaccine%20against%20challenge%20with%20a%20Malaysian%20H5N1%20highly%20pathogenic%20avian%20influenza%20virus.pdf
_version_ 1819295543446208512
score 13.4562235

Ähnliche Einträge