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A method for improving the recovery of fluorescent aromatic compounds (FACs) from fish bile and a kit thereof

The present invention relates to a method and a kit for improving the recovery of fluorescent aromatic compounds (FACs) from fish bile. The method comprising: preparing a volume of bile sample from a fish; adding a volume of enzyme combination and a volume of distilled water to the volume of bile in...

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Główni autorzy: Varnamkhasti, Ali Karami, Christianus, Annie, Shamsuddin, Zulkifli, Ishak, Zamri
Format: Patent
Wydane: 2011
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id oai:psasir.upm.edu.my:33119
record_format eprints
spelling oai:psasir.upm.edu.my:33119 http://psasir.upm.edu.my/id/eprint/33119/ A method for improving the recovery of fluorescent aromatic compounds (FACs) from fish bile and a kit thereof Varnamkhasti, Ali Karami Christianus, Annie Shamsuddin, Zulkifli Ishak, Zamri The present invention relates to a method and a kit for improving the recovery of fluorescent aromatic compounds (FACs) from fish bile. The method comprising: preparing a volume of bile sample from a fish; adding a volume of enzyme combination and a volume of distilled water to the volume of bile in order to obtain a mixture; incubating the mixture; adding a volume of acetone to the mixture; centrifuging the mixture and obtaining a supernatant and a pellet; and recovering the biliary FACs in the supernatant using high performance liquid chromatography with fluorescent detection (HPLC/FL). To increase accuracy of the method, pellets resulted from the centrifugation were resuspended in distilled water and the above-mentioned procedure was repeated but using different ratios of distilled water, enzyme combination, and acetone. The highly accurate result is required for accurate estimation of PAHs concentrations in aquatic environments. In addition, early estimation of low concentrations of PAHs in fish products through fish bile ensures the extra safe consumption of fish products by human and farm animals. 2011-12-09 Patent NonPeerReviewed Annie Christianus (2011) A method for improving the recovery of fluorescent aromatic compounds (FACs) from fish bile and a kit thereof. PI2011005989.
institution UPM IR
collection UPM IR
description The present invention relates to a method and a kit for improving the recovery of fluorescent aromatic compounds (FACs) from fish bile. The method comprising: preparing a volume of bile sample from a fish; adding a volume of enzyme combination and a volume of distilled water to the volume of bile in order to obtain a mixture; incubating the mixture; adding a volume of acetone to the mixture; centrifuging the mixture and obtaining a supernatant and a pellet; and recovering the biliary FACs in the supernatant using high performance liquid chromatography with fluorescent detection (HPLC/FL). To increase accuracy of the method, pellets resulted from the centrifugation were resuspended in distilled water and the above-mentioned procedure was repeated but using different ratios of distilled water, enzyme combination, and acetone. The highly accurate result is required for accurate estimation of PAHs concentrations in aquatic environments. In addition, early estimation of low concentrations of PAHs in fish products through fish bile ensures the extra safe consumption of fish products by human and farm animals.
format Patent
author Varnamkhasti, Ali Karami
Christianus, Annie
Shamsuddin, Zulkifli
Ishak, Zamri
spellingShingle Varnamkhasti, Ali Karami
Christianus, Annie
Shamsuddin, Zulkifli
Ishak, Zamri
A method for improving the recovery of fluorescent aromatic compounds (FACs) from fish bile and a kit thereof
author_facet Varnamkhasti, Ali Karami
Christianus, Annie
Shamsuddin, Zulkifli
Ishak, Zamri
author_sort Varnamkhasti, Ali Karami
title A method for improving the recovery of fluorescent aromatic compounds (FACs) from fish bile and a kit thereof
title_short A method for improving the recovery of fluorescent aromatic compounds (FACs) from fish bile and a kit thereof
title_full A method for improving the recovery of fluorescent aromatic compounds (FACs) from fish bile and a kit thereof
title_fullStr A method for improving the recovery of fluorescent aromatic compounds (FACs) from fish bile and a kit thereof
title_full_unstemmed A method for improving the recovery of fluorescent aromatic compounds (FACs) from fish bile and a kit thereof
title_sort method for improving the recovery of fluorescent aromatic compounds (facs) from fish bile and a kit thereof
publishDate 2011
_version_ 1819295567222669312
score 13.4562235

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