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Optimal conditions for hepatitis B core antigen production in shaked flask fermentation

The effects of various environmental factors such as pH (5, 6, 7, 8 and 9), temperature (30, 37 and 40°C) and rotational speed (150, 200 and 250 rpm) on the growth and the hepatitis B core antigen (HBcAg) production of Escherichia coli W3110IQ were examined in the present study. The highest growth r...

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Main Authors: Tey, Beng Ti, Yong, Kok Hoe, Ong, Hong Puay, Ling, Tau Chuan, Ong, Swee Tin, Tan, Yan Peng, Ariff, Arbakariya, Tan, Wen Siang
Format: Article
Language:English
Published: Korean Society for Biotechnology and Bioengineering 2004
Online Access:http://psasir.upm.edu.my/id/eprint/34502/1/Optimal%20conditions%20for%20hepatitis%20B%20core%20antigen%20production%20in%20shaked%20flask%20fermentation.pdf
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spelling oai:psasir.upm.edu.my:34502 http://psasir.upm.edu.my/id/eprint/34502/ Optimal conditions for hepatitis B core antigen production in shaked flask fermentation Tey, Beng Ti Yong, Kok Hoe Ong, Hong Puay Ling, Tau Chuan Ong, Swee Tin Tan, Yan Peng Ariff, Arbakariya Tan, Wen Siang The effects of various environmental factors such as pH (5, 6, 7, 8 and 9), temperature (30, 37 and 40°C) and rotational speed (150, 200 and 250 rpm) on the growth and the hepatitis B core antigen (HBcAg) production of Escherichia coli W3110IQ were examined in the present study. The highest growth rate is achieved at PH 7, 37°C and at a rotational speed of 250 rpm which is 0.927 h−1. The effect of pH on cell growth is more substantial compared to other parameters; it recorded a 123% different between the highest growth rate (0.927 h−1) at pH 7 and lowest growth at pH 5. The highest protein yield is achieved at pH 9, rotational speed of 250 rpm and 40°C. The yield of protein at pH 7 is 154% higher compared to the lowest yield achieved at pH 5. There is about 28% different of the protein yield for theE. coli cultivated at 250 rpm compared to that at 150 rpm which has the lowest HBcAg yield. The yield of protein at 40°C is 38% higher compared to the lowest yield achieved, at 30°C. Korean Society for Biotechnology and Bioengineering 2004 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34502/1/Optimal%20conditions%20for%20hepatitis%20B%20core%20antigen%20production%20in%20shaked%20flask%20fermentation.pdf Tey, Beng Ti and Yong, Kok Hoe and Ong, Hong Puay and Ling, Tau Chuan and Ong, Swee Tin and Tan, Yan Peng and Ariff, Arbakariya and Tan, Wen Siang (2004) Optimal conditions for hepatitis B core antigen production in shaked flask fermentation. Biotechnology and Bioprocess Engineering, 9 (5). pp. 374-378. ISSN 1226-8372; ESSN: 1976-3816 http://link.springer.com/article/10.1007/BF02933060?view=classic 10.1007/BF02933060
institution UPM IR
collection UPM IR
language English
description The effects of various environmental factors such as pH (5, 6, 7, 8 and 9), temperature (30, 37 and 40°C) and rotational speed (150, 200 and 250 rpm) on the growth and the hepatitis B core antigen (HBcAg) production of Escherichia coli W3110IQ were examined in the present study. The highest growth rate is achieved at PH 7, 37°C and at a rotational speed of 250 rpm which is 0.927 h−1. The effect of pH on cell growth is more substantial compared to other parameters; it recorded a 123% different between the highest growth rate (0.927 h−1) at pH 7 and lowest growth at pH 5. The highest protein yield is achieved at pH 9, rotational speed of 250 rpm and 40°C. The yield of protein at pH 7 is 154% higher compared to the lowest yield achieved at pH 5. There is about 28% different of the protein yield for theE. coli cultivated at 250 rpm compared to that at 150 rpm which has the lowest HBcAg yield. The yield of protein at 40°C is 38% higher compared to the lowest yield achieved, at 30°C.
format Article
author Tey, Beng Ti
Yong, Kok Hoe
Ong, Hong Puay
Ling, Tau Chuan
Ong, Swee Tin
Tan, Yan Peng
Ariff, Arbakariya
Tan, Wen Siang
spellingShingle Tey, Beng Ti
Yong, Kok Hoe
Ong, Hong Puay
Ling, Tau Chuan
Ong, Swee Tin
Tan, Yan Peng
Ariff, Arbakariya
Tan, Wen Siang
Optimal conditions for hepatitis B core antigen production in shaked flask fermentation
author_facet Tey, Beng Ti
Yong, Kok Hoe
Ong, Hong Puay
Ling, Tau Chuan
Ong, Swee Tin
Tan, Yan Peng
Ariff, Arbakariya
Tan, Wen Siang
author_sort Tey, Beng Ti
title Optimal conditions for hepatitis B core antigen production in shaked flask fermentation
title_short Optimal conditions for hepatitis B core antigen production in shaked flask fermentation
title_full Optimal conditions for hepatitis B core antigen production in shaked flask fermentation
title_fullStr Optimal conditions for hepatitis B core antigen production in shaked flask fermentation
title_full_unstemmed Optimal conditions for hepatitis B core antigen production in shaked flask fermentation
title_sort optimal conditions for hepatitis b core antigen production in shaked flask fermentation
publisher Korean Society for Biotechnology and Bioengineering
publishDate 2004
url http://psasir.upm.edu.my/id/eprint/34502/1/Optimal%20conditions%20for%20hepatitis%20B%20core%20antigen%20production%20in%20shaked%20flask%20fermentation.pdf
_version_ 1782720950958030848
score 12.935284

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