Modeling of crude oil biodegradation using two phase partitioning bioreactor
In this work, crude oil biodegradation has been optimized in a solid-liquid two phase partitioning bioreactor (TPPB) by applying a response surface methodology based d-optimal design. Three key factors including phase ratio, substrate concentration in solid organic phase, and sodium chloride concent...
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oai:psasir.upm.edu.my:34548 http://psasir.upm.edu.my/id/eprint/34548/ Modeling of crude oil biodegradation using two phase partitioning bioreactor Ahmadun, Fakhru'l-Razi Peyda, Mazyar Wan Ab Karim Ghani, Wan Azlina Zainal Abidin, Zurina Zakaria, Mohamad Pauzi Moeini, Hassan In this work, crude oil biodegradation has been optimized in a solid-liquid two phase partitioning bioreactor (TPPB) by applying a response surface methodology based d-optimal design. Three key factors including phase ratio, substrate concentration in solid organic phase, and sodium chloride concentration in aqueous phase were taken as independent variables, while the efficiency of the biodegradation of absorbed crude oil on polymer beads was considered to be the dependent variable. Commercial thermoplastic polyurethane (Desmopan®) was used as the solid phase in the TPPB. The designed experiments were carried out batch wise using a mixed acclimatized bacterial consortium. Optimum combinations of key factors with a statistically significant cubic model were used to maximize biodegradation in the TPPB. The validity of the model was successfully verified by the good agreement between the model-predicted and experimental results. When applying the optimum parameters, gas chromatography-mass spectrometry showed a significant reduction in n-alkanes and low molecular weight polycyclic aromatic hydrocarbons. This consequently highlights the practical applicability of TPPB in crude oil biodegradation. Wiley-Blackwell Publishing 2014 Article PeerReviewed Ahmadun, Fakhru'l-Razi and Peyda, Mazyar and Wan Ab Karim Ghani, Wan Azlina and Zainal Abidin, Zurina and Zakaria, Mohamad Pauzi and Moeini, Hassan (2014) Modeling of crude oil biodegradation using two phase partitioning bioreactor. Biotechnology Progress, 30 (4). pp. 797-805. ISSN 8756-7938; ESSN: 1520-6033 10.1002/btpr.1911 |
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In this work, crude oil biodegradation has been optimized in a solid-liquid two phase partitioning bioreactor (TPPB) by applying a response surface methodology based d-optimal design. Three key factors including phase ratio, substrate concentration in solid organic phase, and sodium chloride concentration in aqueous phase were taken as independent variables, while the efficiency of the biodegradation of absorbed crude oil on polymer beads was considered to be the dependent variable. Commercial thermoplastic polyurethane (Desmopan®) was used as the solid phase in the TPPB. The designed experiments were carried out batch wise using a mixed acclimatized bacterial consortium. Optimum combinations of key factors with a statistically significant cubic model were used to maximize biodegradation in the TPPB. The validity of the model was successfully verified by the good agreement between the model-predicted and experimental results. When applying the optimum parameters, gas chromatography-mass spectrometry showed a significant reduction in n-alkanes and low molecular weight polycyclic aromatic hydrocarbons. This consequently highlights the practical applicability of TPPB in crude oil biodegradation. |
| format |
Article |
| author |
Ahmadun, Fakhru'l-Razi Peyda, Mazyar Wan Ab Karim Ghani, Wan Azlina Zainal Abidin, Zurina Zakaria, Mohamad Pauzi Moeini, Hassan |
| spellingShingle |
Ahmadun, Fakhru'l-Razi Peyda, Mazyar Wan Ab Karim Ghani, Wan Azlina Zainal Abidin, Zurina Zakaria, Mohamad Pauzi Moeini, Hassan Modeling of crude oil biodegradation using two phase partitioning bioreactor |
| author_facet |
Ahmadun, Fakhru'l-Razi Peyda, Mazyar Wan Ab Karim Ghani, Wan Azlina Zainal Abidin, Zurina Zakaria, Mohamad Pauzi Moeini, Hassan |
| author_sort |
Ahmadun, Fakhru'l-Razi |
| title |
Modeling of crude oil biodegradation using two phase partitioning bioreactor |
| title_short |
Modeling of crude oil biodegradation using two phase partitioning bioreactor |
| title_full |
Modeling of crude oil biodegradation using two phase partitioning bioreactor |
| title_fullStr |
Modeling of crude oil biodegradation using two phase partitioning bioreactor |
| title_full_unstemmed |
Modeling of crude oil biodegradation using two phase partitioning bioreactor |
| title_sort |
modeling of crude oil biodegradation using two phase partitioning bioreactor |
| publisher |
Wiley-Blackwell Publishing |
| publishDate |
2014 |
| _version_ |
1819295698358632448 |
| score |
13.4562235 |