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Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs)

An embryonic stem cell (ESC) is a good tool to generate neurons in vitro and can be used to mimic neural development in vivo. It has been widely used in research to examine the role of cell signalling during neuronal development, test the effects of drugs on neurons, and generate a large population...

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Main Authors: Liyang, Gao, Abdullah, Syahrilnizam, Rosli, Rozita, Nordin, Norshariza
Format: Article
Published: Penerbit Universiti Sains Malaysia 2014
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id oai:psasir.upm.edu.my:34638
record_format eprints
spelling oai:psasir.upm.edu.my:34638 http://psasir.upm.edu.my/id/eprint/34638/ Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs) Liyang, Gao Abdullah, Syahrilnizam Rosli, Rozita Nordin, Norshariza An embryonic stem cell (ESC) is a good tool to generate neurons in vitro and can be used to mimic neural development in vivo. It has been widely used in research to examine the role of cell signalling during neuronal development, test the effects of drugs on neurons, and generate a large population of functional neurons. So far, a number of protocols have been established to promote the differentiation of ESCs, such as direct and indirect differentiation. One of the widely used protocols to generate neurons is through the spontaneous formation of multicellular aggregates known as embryonic bodies (EBs). However, for some, it is not clear why EB protocol could be the protocol of choice. EB also is known to mimic an early embryo; hence, knowing the similarities between EB and an early embryo is essential, particularly the information on the players that promote the formation of EBs or the aggregation of ESCs. This review paper focuses on these issues and discusses further the generation of neural cells from EBs using a well-known protocol, the 4-/4+ protocol. Penerbit Universiti Sains Malaysia 2014 Article PeerReviewed Liyang, Gao and Abdullah, Syahrilnizam and Rosli, Rozita and Nordin, Norshariza (2014) Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs). Malaysian Journal of Medical Sciences, 21 (5). pp. 8-16. ISSN 1394-195X; ESSN: 2180-4303 http://www.mjms.usm.my/default.asp?tag=14&kod_volume=77
institution UPM IR
collection UPM IR
description An embryonic stem cell (ESC) is a good tool to generate neurons in vitro and can be used to mimic neural development in vivo. It has been widely used in research to examine the role of cell signalling during neuronal development, test the effects of drugs on neurons, and generate a large population of functional neurons. So far, a number of protocols have been established to promote the differentiation of ESCs, such as direct and indirect differentiation. One of the widely used protocols to generate neurons is through the spontaneous formation of multicellular aggregates known as embryonic bodies (EBs). However, for some, it is not clear why EB protocol could be the protocol of choice. EB also is known to mimic an early embryo; hence, knowing the similarities between EB and an early embryo is essential, particularly the information on the players that promote the formation of EBs or the aggregation of ESCs. This review paper focuses on these issues and discusses further the generation of neural cells from EBs using a well-known protocol, the 4-/4+ protocol.
format Article
author Liyang, Gao
Abdullah, Syahrilnizam
Rosli, Rozita
Nordin, Norshariza
spellingShingle Liyang, Gao
Abdullah, Syahrilnizam
Rosli, Rozita
Nordin, Norshariza
Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs)
author_facet Liyang, Gao
Abdullah, Syahrilnizam
Rosli, Rozita
Nordin, Norshariza
author_sort Liyang, Gao
title Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs)
title_short Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs)
title_full Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs)
title_fullStr Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs)
title_full_unstemmed Neural commitment of embryonic stem cells through the formation of embryoid bodies (EBs)
title_sort neural commitment of embryonic stem cells through the formation of embryoid bodies (ebs)
publisher Penerbit Universiti Sains Malaysia
publishDate 2014
_version_ 1819295706935984128
score 13.4562235

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