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Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia

Aeromonas infections are becoming a major risk factor in commercial aquaculture and it has been reported that a wide variety of fish and shellfish species are susceptible to this infection. In this study, 3 isolates of Aeromonas hydrophila were isolated from giant freshwater prawn (Macrobrachium ros...

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Main Authors: Sabili, Abdolnabi, Md Yasin, Ina Salwany, Mohd. Daud, Hassan, Shamsudin, Mariana Nor, Abdelhadi, Yasser
Format: Article
Language:English
Published: Iranian Fisheries Science Research Institute 2015
Online Access:http://psasir.upm.edu.my/id/eprint/34838/1/Pathogenicity%20of%20Aeromonas%20hydrophila%20in%20giant%20freshwater%20prawn.pdf
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spelling oai:psasir.upm.edu.my:34838 http://psasir.upm.edu.my/id/eprint/34838/ Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia Sabili, Abdolnabi Md Yasin, Ina Salwany Mohd. Daud, Hassan Shamsudin, Mariana Nor Abdelhadi, Yasser Aeromonas infections are becoming a major risk factor in commercial aquaculture and it has been reported that a wide variety of fish and shellfish species are susceptible to this infection. In this study, 3 isolates of Aeromonas hydrophila were isolated from giant freshwater prawn (Macrobrachium rosenbergii) cultured in Kuala Pilah Simbilan Nigri in East Malaysia. Conventional and rapid identification systems (API 20E strips) were used for preliminary identification based on the biochemical characters of the isolated bacteria. On the other hand, polymerase chain reaction (PCR) using the universal primer; 16S rRNA, was done as an accurate and confirmatory identification. The virulence of A. hydrophila was determined using a pathogenicity test via I/M injection. The results revealed that the isolated bacteria were identified as A. hydrophila that revealed a high degree of similarity (98%) to the NCBI or Genbank database. Based on pathogenicity test results, LD50 was determined as 1×106 CFU/50µl, while 1×107 CFU/50µl induced 100% mortality in the experimentally injected prawns. Histopathological changes were found in several organs including gill, hepatopancreas and heart. Those changes were mainly, melanisation, tissue erosion and necrosis, infiltration and hyperplasia of gill lamellae and mild or massive haemocyte reaction in the infected organs. Iranian Fisheries Science Research Institute 2015 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34838/1/Pathogenicity%20of%20Aeromonas%20hydrophila%20in%20giant%20freshwater%20prawn.pdf Sabili, Abdolnabi and Md Yasin, Ina Salwany and Mohd. Daud, Hassan and Shamsudin, Mariana Nor and Abdelhadi, Yasser (2015) Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia. Iranian Journal of Fisheries Sciences , 14 (1). pp. 232-245. ISSN 1562-2916 http://www.jifro.ir/browse.php?a_id=1857&sid=1&slc_lang=en
institution UPM IR
collection UPM IR
language English
description Aeromonas infections are becoming a major risk factor in commercial aquaculture and it has been reported that a wide variety of fish and shellfish species are susceptible to this infection. In this study, 3 isolates of Aeromonas hydrophila were isolated from giant freshwater prawn (Macrobrachium rosenbergii) cultured in Kuala Pilah Simbilan Nigri in East Malaysia. Conventional and rapid identification systems (API 20E strips) were used for preliminary identification based on the biochemical characters of the isolated bacteria. On the other hand, polymerase chain reaction (PCR) using the universal primer; 16S rRNA, was done as an accurate and confirmatory identification. The virulence of A. hydrophila was determined using a pathogenicity test via I/M injection. The results revealed that the isolated bacteria were identified as A. hydrophila that revealed a high degree of similarity (98%) to the NCBI or Genbank database. Based on pathogenicity test results, LD50 was determined as 1×106 CFU/50µl, while 1×107 CFU/50µl induced 100% mortality in the experimentally injected prawns. Histopathological changes were found in several organs including gill, hepatopancreas and heart. Those changes were mainly, melanisation, tissue erosion and necrosis, infiltration and hyperplasia of gill lamellae and mild or massive haemocyte reaction in the infected organs.
format Article
author Sabili, Abdolnabi
Md Yasin, Ina Salwany
Mohd. Daud, Hassan
Shamsudin, Mariana Nor
Abdelhadi, Yasser
spellingShingle Sabili, Abdolnabi
Md Yasin, Ina Salwany
Mohd. Daud, Hassan
Shamsudin, Mariana Nor
Abdelhadi, Yasser
Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia
author_facet Sabili, Abdolnabi
Md Yasin, Ina Salwany
Mohd. Daud, Hassan
Shamsudin, Mariana Nor
Abdelhadi, Yasser
author_sort Sabili, Abdolnabi
title Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia
title_short Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia
title_full Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia
title_fullStr Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia
title_full_unstemmed Pathogenicity of Aeromonas hydrophila in giant freshwater prawn; Macrobrachium rosenbergii, cultured in East Malaysia
title_sort pathogenicity of aeromonas hydrophila in giant freshwater prawn; macrobrachium rosenbergii, cultured in east malaysia
publisher Iranian Fisheries Science Research Institute
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/34838/1/Pathogenicity%20of%20Aeromonas%20hydrophila%20in%20giant%20freshwater%20prawn.pdf
_version_ 1819295726227685376
score 13.4562235

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