A three-dimensional collagen construct to model lipopolysaccharide-induced activation of BV2 microglia

Background: We report a novel method of culturing microglia in three dimension (3D) using collagen as a substrate. By culturing microglia within a matrix, we aim to emulate the physical state of microglia embedded within parenchyma. Methods: BV2 microglia cell suspensions were prepared with type I c...

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Autores Principales: Haw, Randy Tatt Yhew, Tong, Chih Kong, Yew, Andrew, Lee, Han Chung, Phillips, James B., Vidyadaran, Sharmili
Formato: Artículo
Lenguaje:English
Publicado: BioMed Central 2014
Acceso en línea:http://psasir.upm.edu.my/id/eprint/36302/1/A%20three.pdf
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