A three-dimensional collagen construct to model lipopolysaccharide-induced activation of BV2 microglia
Background: We report a novel method of culturing microglia in three dimension (3D) using collagen as a substrate. By culturing microglia within a matrix, we aim to emulate the physical state of microglia embedded within parenchyma. Methods: BV2 microglia cell suspensions were prepared with type I c...
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