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Alteration in lymphocyte responses, cytokine and chemokine profiles in chickens infected with genotype VII and VIII velogenic Newcastle disease virus

Newcastle disease (ND) is a highly contagious avian disease and one of the major causes of economic losses in the poultry industry. The emergence of virulent NDV genotypes and repeated outbreaks of NDV in vaccinated chickens have raised the need for fundamental studies on the virus–host interactions...

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Principais autores: Rasoli, Mehdi, Yeap, Swee Keong, Tan, Sheau Wei, Moeini, Hassan, Ideris, Aini, Bejo, Mohd Hair, Mohamed Alitheen, Noorjahan Banu, Kaiser, Pete, Omar, Abdul Rahman
Formato: Atigo
Idioma:English
Publicado em: Elsevier 2014
Acesso em linha:http://psasir.upm.edu.my/id/eprint/36368/1/Alteration%20in%20lymphocyte%20responses.pdf
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spelling oai:psasir.upm.edu.my:36368 http://psasir.upm.edu.my/id/eprint/36368/ Alteration in lymphocyte responses, cytokine and chemokine profiles in chickens infected with genotype VII and VIII velogenic Newcastle disease virus Rasoli, Mehdi Yeap, Swee Keong Tan, Sheau Wei Moeini, Hassan Ideris, Aini Bejo, Mohd Hair Mohamed Alitheen, Noorjahan Banu Kaiser, Pete Omar, Abdul Rahman Newcastle disease (ND) is a highly contagious avian disease and one of the major causes of economic losses in the poultry industry. The emergence of virulent NDV genotypes and repeated outbreaks of NDV in vaccinated chickens have raised the need for fundamental studies on the virus–host interactions. In this study, the profiles of B and T lymphocytes and macrophages and differential expression of 26 immune-related genes in the spleen of specific-pathogen-free (SPF) chickens, infected with either the velogenic genotype VII NDV strain IBS002 or the genotype VIII NDV strain AF2240, were evaluated. A significant reduction in T lymphocyte population and an increase in the infiltration of IgM+ B cells and KUL01+ macrophages were detected in the infected spleens at 1, 3 and 4 days post-infection (dpi) (P < 0.05). The gene expression profiles showed an up-regulation of CCLi3, CXCLi1, CXCLi2 (IL-8), IFN-γ, IL-12α, IL-18, IL-1β, IL-6, iNOS, TLR7, MHCI, IL-17F and TNFSF13B (P < 0.05). However, these two genotypes showed different cytokine expression patterns and viral load. IBS002 showed higher viral load than AF2240 in spleen at 3 and 4 dpi and caused a more rapid up-regulation of CXCLi2, IFN-γ, IL-12α, IL-18, IL-1β, iNOS and IL-10 at 3 dpi. Meanwhile, the expression levels of CCLI3, CXCLi1, IFN-γ, IL-12α, IL-1β and iNOS genes were significantly higher in AF2240 at 4 dpi. In addition, the expression levels of IL-10 were significantly higher in the IBS002-infected chickens at 3 and 4 dpi. Hence, infection with velogenic genotype VII and VIII NDV induced different viral load and production of cytokines and chemokines associated with inflammatory reactions. Elsevier 2014-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36368/1/Alteration%20in%20lymphocyte%20responses.pdf Rasoli, Mehdi and Yeap, Swee Keong and Tan, Sheau Wei and Moeini, Hassan and Ideris, Aini and Bejo, Mohd Hair and Mohamed Alitheen, Noorjahan Banu and Kaiser, Pete and Omar, Abdul Rahman (2014) Alteration in lymphocyte responses, cytokine and chemokine profiles in chickens infected with genotype VII and VIII velogenic Newcastle disease virus. Comparative Immunology, Microbiology and Infectious Diseases, 37 (1). pp. 11-21. ISSN 0147-9571; ESSN: 1878-1667 10.1016/j.cimid.2013.10.003
institution UPM IR
collection UPM IR
language English
description Newcastle disease (ND) is a highly contagious avian disease and one of the major causes of economic losses in the poultry industry. The emergence of virulent NDV genotypes and repeated outbreaks of NDV in vaccinated chickens have raised the need for fundamental studies on the virus–host interactions. In this study, the profiles of B and T lymphocytes and macrophages and differential expression of 26 immune-related genes in the spleen of specific-pathogen-free (SPF) chickens, infected with either the velogenic genotype VII NDV strain IBS002 or the genotype VIII NDV strain AF2240, were evaluated. A significant reduction in T lymphocyte population and an increase in the infiltration of IgM+ B cells and KUL01+ macrophages were detected in the infected spleens at 1, 3 and 4 days post-infection (dpi) (P < 0.05). The gene expression profiles showed an up-regulation of CCLi3, CXCLi1, CXCLi2 (IL-8), IFN-γ, IL-12α, IL-18, IL-1β, IL-6, iNOS, TLR7, MHCI, IL-17F and TNFSF13B (P < 0.05). However, these two genotypes showed different cytokine expression patterns and viral load. IBS002 showed higher viral load than AF2240 in spleen at 3 and 4 dpi and caused a more rapid up-regulation of CXCLi2, IFN-γ, IL-12α, IL-18, IL-1β, iNOS and IL-10 at 3 dpi. Meanwhile, the expression levels of CCLI3, CXCLi1, IFN-γ, IL-12α, IL-1β and iNOS genes were significantly higher in AF2240 at 4 dpi. In addition, the expression levels of IL-10 were significantly higher in the IBS002-infected chickens at 3 and 4 dpi. Hence, infection with velogenic genotype VII and VIII NDV induced different viral load and production of cytokines and chemokines associated with inflammatory reactions.
format Article
author Rasoli, Mehdi
Yeap, Swee Keong
Tan, Sheau Wei
Moeini, Hassan
Ideris, Aini
Bejo, Mohd Hair
Mohamed Alitheen, Noorjahan Banu
Kaiser, Pete
Omar, Abdul Rahman
spellingShingle Rasoli, Mehdi
Yeap, Swee Keong
Tan, Sheau Wei
Moeini, Hassan
Ideris, Aini
Bejo, Mohd Hair
Mohamed Alitheen, Noorjahan Banu
Kaiser, Pete
Omar, Abdul Rahman
Alteration in lymphocyte responses, cytokine and chemokine profiles in chickens infected with genotype VII and VIII velogenic Newcastle disease virus
author_facet Rasoli, Mehdi
Yeap, Swee Keong
Tan, Sheau Wei
Moeini, Hassan
Ideris, Aini
Bejo, Mohd Hair
Mohamed Alitheen, Noorjahan Banu
Kaiser, Pete
Omar, Abdul Rahman
author_sort Rasoli, Mehdi
title Alteration in lymphocyte responses, cytokine and chemokine profiles in chickens infected with genotype VII and VIII velogenic Newcastle disease virus
title_short Alteration in lymphocyte responses, cytokine and chemokine profiles in chickens infected with genotype VII and VIII velogenic Newcastle disease virus
title_full Alteration in lymphocyte responses, cytokine and chemokine profiles in chickens infected with genotype VII and VIII velogenic Newcastle disease virus
title_fullStr Alteration in lymphocyte responses, cytokine and chemokine profiles in chickens infected with genotype VII and VIII velogenic Newcastle disease virus
title_full_unstemmed Alteration in lymphocyte responses, cytokine and chemokine profiles in chickens infected with genotype VII and VIII velogenic Newcastle disease virus
title_sort alteration in lymphocyte responses, cytokine and chemokine profiles in chickens infected with genotype vii and viii velogenic newcastle disease virus
publisher Elsevier
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/36368/1/Alteration%20in%20lymphocyte%20responses.pdf
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score 13.4562235

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