Cover Image

Comparison between phage-ELISA and phage dot-blot assay methods for the detection of hepatitis B surface antigen and its antibodies in human serum

A modified phage-enzyme link immunosorbent assay (phage-ELISA) and a phage dot-blot assay specific for hepatitis B surface antigen (HBsAg) and its antibody were developed by using phage display technology. The phage-ELISA and phage dot-blot assays enabled to detect HBsAg and anti-HBsAg in human sera...

Full description

Saved in:
Bibliographic Details
Main Authors: Tan, Geok Hun, Tan, Wen Siang
Format: Article
Language:English
Published: Lifescience Global 2014
Online Access:http://psasir.upm.edu.my/id/eprint/36834/1/Comparison%20between%20phage-ELISA%20and%20phage%20dot-blot%20assay%20methods%20for%20the%20detection%20of%20hepatitis%20B%20surface%20antigen%20and%20its%20antibodies%20in%20human%20serum.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:psasir.upm.edu.my:36834
record_format eprints
spelling oai:psasir.upm.edu.my:36834 http://psasir.upm.edu.my/id/eprint/36834/ Comparison between phage-ELISA and phage dot-blot assay methods for the detection of hepatitis B surface antigen and its antibodies in human serum Tan, Geok Hun Tan, Wen Siang A modified phage-enzyme link immunosorbent assay (phage-ELISA) and a phage dot-blot assay specific for hepatitis B surface antigen (HBsAg) and its antibody were developed by using phage display technology. The phage-ELISA and phage dot-blot assays enabled to detect HBsAg and anti-HBsAg in human sera, and compatible to commercial detection kit. The fusion phages were immobilized onto microtiter plate wells and nitrocellulose membrane sheets, then blocked with 10% milk diluent, and added with human serum at dilution of 1:5000. The absorbance at 405 nm was determined once the colour changes formed. The same human serum also applied on the commercial diagnostic kit for comparison. The statistical analysis was carried out using ANOVA and T Test (LSD) for variable comparison between phage-ELISA and phage dot-blot assays. Based on these studies, the phage-ELISA was found to be more sensitive compared to phage dot-blot assay as the detection of HBsAg in human sera was about 80% as compared to 51.7% by using phage dot-blot assay. Meanwhile, the sensitivity for detection of anti-HBsAg by using phage-ELISA was slightly higher which showed about 83.3%. However, the sensitivity of the assay was dropped almost half when using phage dot-blot assay. Therefore, they are practical to be used as a reliable alternative way for the detection HBsAg and its antibody in human sera. Lifescience Global 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36834/1/Comparison%20between%20phage-ELISA%20and%20phage%20dot-blot%20assay%20methods%20for%20the%20detection%20of%20hepatitis%20B%20surface%20antigen%20and%20its%20antibodies%20in%20human%20serum.pdf Tan, Geok Hun and Tan, Wen Siang (2014) Comparison between phage-ELISA and phage dot-blot assay methods for the detection of hepatitis B surface antigen and its antibodies in human serum. International Journal of Biotechnology for Wellness Industries, 3 (4). pp. 128-135. ISSN 1927-3037 http://www.lifescienceglobal.com/pms/index.php/ijbwi/article/view/2653
institution UPM IR
collection UPM IR
language English
description A modified phage-enzyme link immunosorbent assay (phage-ELISA) and a phage dot-blot assay specific for hepatitis B surface antigen (HBsAg) and its antibody were developed by using phage display technology. The phage-ELISA and phage dot-blot assays enabled to detect HBsAg and anti-HBsAg in human sera, and compatible to commercial detection kit. The fusion phages were immobilized onto microtiter plate wells and nitrocellulose membrane sheets, then blocked with 10% milk diluent, and added with human serum at dilution of 1:5000. The absorbance at 405 nm was determined once the colour changes formed. The same human serum also applied on the commercial diagnostic kit for comparison. The statistical analysis was carried out using ANOVA and T Test (LSD) for variable comparison between phage-ELISA and phage dot-blot assays. Based on these studies, the phage-ELISA was found to be more sensitive compared to phage dot-blot assay as the detection of HBsAg in human sera was about 80% as compared to 51.7% by using phage dot-blot assay. Meanwhile, the sensitivity for detection of anti-HBsAg by using phage-ELISA was slightly higher which showed about 83.3%. However, the sensitivity of the assay was dropped almost half when using phage dot-blot assay. Therefore, they are practical to be used as a reliable alternative way for the detection HBsAg and its antibody in human sera.
format Article
author Tan, Geok Hun
Tan, Wen Siang
spellingShingle Tan, Geok Hun
Tan, Wen Siang
Comparison between phage-ELISA and phage dot-blot assay methods for the detection of hepatitis B surface antigen and its antibodies in human serum
author_facet Tan, Geok Hun
Tan, Wen Siang
author_sort Tan, Geok Hun
title Comparison between phage-ELISA and phage dot-blot assay methods for the detection of hepatitis B surface antigen and its antibodies in human serum
title_short Comparison between phage-ELISA and phage dot-blot assay methods for the detection of hepatitis B surface antigen and its antibodies in human serum
title_full Comparison between phage-ELISA and phage dot-blot assay methods for the detection of hepatitis B surface antigen and its antibodies in human serum
title_fullStr Comparison between phage-ELISA and phage dot-blot assay methods for the detection of hepatitis B surface antigen and its antibodies in human serum
title_full_unstemmed Comparison between phage-ELISA and phage dot-blot assay methods for the detection of hepatitis B surface antigen and its antibodies in human serum
title_sort comparison between phage-elisa and phage dot-blot assay methods for the detection of hepatitis b surface antigen and its antibodies in human serum
publisher Lifescience Global
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/36834/1/Comparison%20between%20phage-ELISA%20and%20phage%20dot-blot%20assay%20methods%20for%20the%20detection%20of%20hepatitis%20B%20surface%20antigen%20and%20its%20antibodies%20in%20human%20serum.pdf
_version_ 1819295921641357312
score 13.4562235

Similar Items