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Germinated brown rice alters Aβ(1-42) aggregation and modulates Alzheimer's disease-related genes in differentiated human SH-SY5Y cells

The pathogenesis of Alzheimer’s disease involves complex etiological factors, of which the deposition of beta-amyloid (Aβ) protein and oxidative stress have been strongly implicated. We explored the effects of H2O2, which is a precursor for highly reactive hydroxyl radicals, on neurotoxicity and gen...

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Main Authors: Azmi, Nur Hanisah, Ismail, Maznah, Ismail, Norsharina, Imam, Mustapha Umar, Mohamed Alitheen, Noorjahan Banu, Abdullah, Maizaton Atmadini
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2015
Online Access:http://psasir.upm.edu.my/id/eprint/37599/1/37599.pdf
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spelling oai:psasir.upm.edu.my:37599 http://psasir.upm.edu.my/id/eprint/37599/ Germinated brown rice alters Aβ(1-42) aggregation and modulates Alzheimer's disease-related genes in differentiated human SH-SY5Y cells Azmi, Nur Hanisah Ismail, Maznah Ismail, Norsharina Imam, Mustapha Umar Mohamed Alitheen, Noorjahan Banu Abdullah, Maizaton Atmadini The pathogenesis of Alzheimer’s disease involves complex etiological factors, of which the deposition of beta-amyloid (Aβ) protein and oxidative stress have been strongly implicated. We explored the effects of H2O2, which is a precursor for highly reactive hydroxyl radicals, on neurotoxicity and genes related to AD on neuronal cells. Candidate bioactive compounds responsible for the effects were quantified using HPLC-DAD. Additionally, the effects of germinated brown rice (GBR) on the morphology of Aβ(1-42) were assessed by Transmission Electron Microscopy and its regulatory effects on gene expressions were explored. The results showed that GBR extract had several phenolic compounds and γ-oryzanol and altered the structure of Aβ(1-42) suggesting an antiamyloidogenic effect. GBR was also able to attenuate the oxidative effects of H2O2 as implied by reduced LDH release and intracellular ROS generation. Furthermore, gene expression analyses showed that the neuroprotective effects of GBR were partly mediated through transcriptional regulation of multiple genes including Presenilins, APP, BACE1, BACE2, ADAM10, Neprilysin, and LRP1. Our findings showed that GBR exhibited neuroprotective properties via transcriptional regulation of APP metabolism with potential impact on Aβ aggregation. These findings can have important implications for the management of neurodegenerative diseases like AD and are worth exploring further. Hindawi Publishing Corporation 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37599/1/37599.pdf Azmi, Nur Hanisah and Ismail, Maznah and Ismail, Norsharina and Imam, Mustapha Umar and Mohamed Alitheen, Noorjahan Banu and Abdullah, Maizaton Atmadini (2015) Germinated brown rice alters Aβ(1-42) aggregation and modulates Alzheimer's disease-related genes in differentiated human SH-SY5Y cells. Evidence-Based Complementary and Alternative Medicine, 2015. art. no. 153684. pp. 1-12. ISSN 1741-427X; ESSN: 1741-4288 http://www.hindawi.com/journals/ecam/2015/153684/abs/ 10.1155/2015/153684
institution UPM IR
collection UPM IR
language English
description The pathogenesis of Alzheimer’s disease involves complex etiological factors, of which the deposition of beta-amyloid (Aβ) protein and oxidative stress have been strongly implicated. We explored the effects of H2O2, which is a precursor for highly reactive hydroxyl radicals, on neurotoxicity and genes related to AD on neuronal cells. Candidate bioactive compounds responsible for the effects were quantified using HPLC-DAD. Additionally, the effects of germinated brown rice (GBR) on the morphology of Aβ(1-42) were assessed by Transmission Electron Microscopy and its regulatory effects on gene expressions were explored. The results showed that GBR extract had several phenolic compounds and γ-oryzanol and altered the structure of Aβ(1-42) suggesting an antiamyloidogenic effect. GBR was also able to attenuate the oxidative effects of H2O2 as implied by reduced LDH release and intracellular ROS generation. Furthermore, gene expression analyses showed that the neuroprotective effects of GBR were partly mediated through transcriptional regulation of multiple genes including Presenilins, APP, BACE1, BACE2, ADAM10, Neprilysin, and LRP1. Our findings showed that GBR exhibited neuroprotective properties via transcriptional regulation of APP metabolism with potential impact on Aβ aggregation. These findings can have important implications for the management of neurodegenerative diseases like AD and are worth exploring further.
format Article
author Azmi, Nur Hanisah
Ismail, Maznah
Ismail, Norsharina
Imam, Mustapha Umar
Mohamed Alitheen, Noorjahan Banu
Abdullah, Maizaton Atmadini
spellingShingle Azmi, Nur Hanisah
Ismail, Maznah
Ismail, Norsharina
Imam, Mustapha Umar
Mohamed Alitheen, Noorjahan Banu
Abdullah, Maizaton Atmadini
Germinated brown rice alters Aβ(1-42) aggregation and modulates Alzheimer's disease-related genes in differentiated human SH-SY5Y cells
author_facet Azmi, Nur Hanisah
Ismail, Maznah
Ismail, Norsharina
Imam, Mustapha Umar
Mohamed Alitheen, Noorjahan Banu
Abdullah, Maizaton Atmadini
author_sort Azmi, Nur Hanisah
title Germinated brown rice alters Aβ(1-42) aggregation and modulates Alzheimer's disease-related genes in differentiated human SH-SY5Y cells
title_short Germinated brown rice alters Aβ(1-42) aggregation and modulates Alzheimer's disease-related genes in differentiated human SH-SY5Y cells
title_full Germinated brown rice alters Aβ(1-42) aggregation and modulates Alzheimer's disease-related genes in differentiated human SH-SY5Y cells
title_fullStr Germinated brown rice alters Aβ(1-42) aggregation and modulates Alzheimer's disease-related genes in differentiated human SH-SY5Y cells
title_full_unstemmed Germinated brown rice alters Aβ(1-42) aggregation and modulates Alzheimer's disease-related genes in differentiated human SH-SY5Y cells
title_sort germinated brown rice alters aβ(1-42) aggregation and modulates alzheimer's disease-related genes in differentiated human sh-sy5y cells
publisher Hindawi Publishing Corporation
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/37599/1/37599.pdf
_version_ 1782721283332505600
score 12.935284

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