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In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)

The current in vitro study was designed to investigate the anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A (BA), a chalcone derivative of known structure isolated from Boesenbergia rotunda. Human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29), non-small cell...

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Main Authors: Mohd Isa, Noorain, Abdelwahab, Siddig Ibrahim, Mohan, Syam, Abdul, Ahmad Bustaman, Sukari, Mohd Aspollah, Taha, Manal Mohamed Elhassan, Syam, Suvitha, Mohd Fauzi, Putri Narrima, Cheah, Shiau Chuen, Ahmad, Syahida, Mustafa, Mohammad Rais
Format: Article
Language:English
Published: Associacao Brasileira de Divulgacao Cientifica 2012
Online Access:http://psasir.upm.edu.my/id/eprint/40428/1/In%20vitro%20anti-inflammatory%2C%20cytotoxic%20and%20antioxidant%20activities%20of%20boesenbergin%20A%2C%20a%20chalcone%20isolated%20from%20Boesenbergia%20rotunda%20%28L.%29%20%28fingerroot%29.pdf
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spelling oai:psasir.upm.edu.my:40428 http://psasir.upm.edu.my/id/eprint/40428/ In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot) Mohd Isa, Noorain Abdelwahab, Siddig Ibrahim Mohan, Syam Abdul, Ahmad Bustaman Sukari, Mohd Aspollah Taha, Manal Mohamed Elhassan Syam, Suvitha Mohd Fauzi, Putri Narrima Cheah, Shiau Chuen Ahmad, Syahida Mustafa, Mohammad Rais The current in vitro study was designed to investigate the anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A (BA), a chalcone derivative of known structure isolated from Boesenbergia rotunda. Human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29), non-small cell lung cancer (A549), prostate adenocarcinoma (PC3), and normal hepatic cells (WRL-68) were used to evaluate the cytotoxicity of BA using the MTT assay. The antioxidant activity of BA was assessed by the ORAC assay and compared to quercetin as a standard reference antioxidant. ORAC results are reported as the equivalent concentration of Trolox that produces the same level of antioxidant activity as the sample tested at 20 µg/mL. The toxic effect of BA on different cell types, reported as IC50, yielded 20.22 ± 3.15, 10.69 ± 2.64, 20.31 ± 1.34, 94.10 ± 1.19, and 9.324 ± 0.24 µg/mL for A549, PC3, HepG2, HT-29, and WRL-68, respectively. BA displayed considerable antioxidant activity, when the results of ORAC assay were reported as Trolox equivalents. BA (20 µg/mL) and quercetin (5 µg/mL) were equivalent to a Trolox concentration of 11.91 ± 0.23 and 160.32 ± 2.75 µM, respectively. Moreover, the anti-inflammatory activity of BA was significant at 12.5 to 50 µg/mL and without any significant cytotoxicity for the murine macrophage cell line RAW 264.7 at 50 µg/mL. The significant biological activities observed in this study indicated that BA may be one of the agents responsible for the reported biological activities of B. rotunda crude extract. Associacao Brasileira de Divulgacao Cientifica 2012-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40428/1/In%20vitro%20anti-inflammatory%2C%20cytotoxic%20and%20antioxidant%20activities%20of%20boesenbergin%20A%2C%20a%20chalcone%20isolated%20from%20Boesenbergia%20rotunda%20%28L.%29%20%28fingerroot%29.pdf Mohd Isa, Noorain and Abdelwahab, Siddig Ibrahim and Mohan, Syam and Abdul, Ahmad Bustaman and Sukari, Mohd Aspollah and Taha, Manal Mohamed Elhassan and Syam, Suvitha and Mohd Fauzi, Putri Narrima and Cheah, Shiau Chuen and Ahmad, Syahida and Mustafa, Mohammad Rais (2012) In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot). Brazilian Journal of Medical and Biological Research, 45 (6). pp. 524-530. ISSN 0100-879X; ESSN: 1678-4510 http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-879X2012000600008&lng=en&nrm=iso&tlng=en 10.1590/S0100-879X2012007500022
institution UPM IR
collection UPM IR
language English
description The current in vitro study was designed to investigate the anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A (BA), a chalcone derivative of known structure isolated from Boesenbergia rotunda. Human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29), non-small cell lung cancer (A549), prostate adenocarcinoma (PC3), and normal hepatic cells (WRL-68) were used to evaluate the cytotoxicity of BA using the MTT assay. The antioxidant activity of BA was assessed by the ORAC assay and compared to quercetin as a standard reference antioxidant. ORAC results are reported as the equivalent concentration of Trolox that produces the same level of antioxidant activity as the sample tested at 20 µg/mL. The toxic effect of BA on different cell types, reported as IC50, yielded 20.22 ± 3.15, 10.69 ± 2.64, 20.31 ± 1.34, 94.10 ± 1.19, and 9.324 ± 0.24 µg/mL for A549, PC3, HepG2, HT-29, and WRL-68, respectively. BA displayed considerable antioxidant activity, when the results of ORAC assay were reported as Trolox equivalents. BA (20 µg/mL) and quercetin (5 µg/mL) were equivalent to a Trolox concentration of 11.91 ± 0.23 and 160.32 ± 2.75 µM, respectively. Moreover, the anti-inflammatory activity of BA was significant at 12.5 to 50 µg/mL and without any significant cytotoxicity for the murine macrophage cell line RAW 264.7 at 50 µg/mL. The significant biological activities observed in this study indicated that BA may be one of the agents responsible for the reported biological activities of B. rotunda crude extract.
format Article
author Mohd Isa, Noorain
Abdelwahab, Siddig Ibrahim
Mohan, Syam
Abdul, Ahmad Bustaman
Sukari, Mohd Aspollah
Taha, Manal Mohamed Elhassan
Syam, Suvitha
Mohd Fauzi, Putri Narrima
Cheah, Shiau Chuen
Ahmad, Syahida
Mustafa, Mohammad Rais
spellingShingle Mohd Isa, Noorain
Abdelwahab, Siddig Ibrahim
Mohan, Syam
Abdul, Ahmad Bustaman
Sukari, Mohd Aspollah
Taha, Manal Mohamed Elhassan
Syam, Suvitha
Mohd Fauzi, Putri Narrima
Cheah, Shiau Chuen
Ahmad, Syahida
Mustafa, Mohammad Rais
In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)
author_facet Mohd Isa, Noorain
Abdelwahab, Siddig Ibrahim
Mohan, Syam
Abdul, Ahmad Bustaman
Sukari, Mohd Aspollah
Taha, Manal Mohamed Elhassan
Syam, Suvitha
Mohd Fauzi, Putri Narrima
Cheah, Shiau Chuen
Ahmad, Syahida
Mustafa, Mohammad Rais
author_sort Mohd Isa, Noorain
title In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)
title_short In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)
title_full In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)
title_fullStr In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)
title_full_unstemmed In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)
title_sort in vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin a, a chalcone isolated from boesenbergia rotunda (l.) (fingerroot)
publisher Associacao Brasileira de Divulgacao Cientifica
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/40428/1/In%20vitro%20anti-inflammatory%2C%20cytotoxic%20and%20antioxidant%20activities%20of%20boesenbergin%20A%2C%20a%20chalcone%20isolated%20from%20Boesenbergia%20rotunda%20%28L.%29%20%28fingerroot%29.pdf
_version_ 1782721638198935552
score 12.935284

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