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Derivation of an endogenous small RNA from double-stranded Sox4 sense and natural antisense transcripts in the mouse brain

Natural antisense transcripts (NATs) are involved in cellular development and regulatory processes. Multiple NATs at the Sox4 gene locus are spatiotemporally regulated throughout murine cerebral corticogenesis. In the study, we evaluated the potential functional role of Sox4 NATs at Sox4 gene locus....

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Main Authors: Ling, King Hwa, Brautigan, Peter J., Moore, Sarah, Fraser, Rachel, Cheah, Pike See, Raison, Joy M., Babic, Milena, Lee, Young Kyung, Daish, Tasman, Mattiske, Deidre M., Mann, Jeffrey R., Adelson, David L., Thomas, Paul Q., Hahn, Christopher N., Scott, Hamish S.
Format: Article
Language:English
Published: Elsevier 2016
Online Access:http://psasir.upm.edu.my/id/eprint/43190/1/Derivation%20of%20an%20endogenous%20small%20RNA%20from%20double-stranded%20Sox4%20sense%20and%20natural%20antisense%20transcripts%20in%20the%20mouse%20brain.pdf
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spelling oai:psasir.upm.edu.my:43190 http://psasir.upm.edu.my/id/eprint/43190/ Derivation of an endogenous small RNA from double-stranded Sox4 sense and natural antisense transcripts in the mouse brain Ling, King Hwa Brautigan, Peter J. Moore, Sarah Fraser, Rachel Cheah, Pike See Raison, Joy M. Babic, Milena Lee, Young Kyung Daish, Tasman Mattiske, Deidre M. Mann, Jeffrey R. Adelson, David L. Thomas, Paul Q. Hahn, Christopher N. Scott, Hamish S. Natural antisense transcripts (NATs) are involved in cellular development and regulatory processes. Multiple NATs at the Sox4 gene locus are spatiotemporally regulated throughout murine cerebral corticogenesis. In the study, we evaluated the potential functional role of Sox4 NATs at Sox4 gene locus. We demonstrated Sox4 sense and NATs formed dsRNA aggregates in the cytoplasm of brain cells. Over expression of Sox4 NATs in NIH/3T3 cells generally did not alter the level of Sox4 mRNA expression or protein translation. Upregulation of a Sox4 NAT known as Sox4ot1 led to the production of a novel small RNA, Sox4_sir3. Its biogenesis is Dicer1-dependent and has characteristics resemble piRNA. Expression of Sox4_sir3 was observed in the marginal and germinative zones of the developing and postnatal brains suggesting a potential role in regulating neurogenesis. We proposed that Sox4 sense-NATs serve as Dicer1-dependent templates to produce a novel endo-siRNA- or piRNA-like Sox4_sir3. Elsevier 2016-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43190/1/Derivation%20of%20an%20endogenous%20small%20RNA%20from%20double-stranded%20Sox4%20sense%20and%20natural%20antisense%20transcripts%20in%20the%20mouse%20brain.pdf Ling, King Hwa and Brautigan, Peter J. and Moore, Sarah and Fraser, Rachel and Cheah, Pike See and Raison, Joy M. and Babic, Milena and Lee, Young Kyung and Daish, Tasman and Mattiske, Deidre M. and Mann, Jeffrey R. and Adelson, David L. and Thomas, Paul Q. and Hahn, Christopher N. and Scott, Hamish S. (2016) Derivation of an endogenous small RNA from double-stranded Sox4 sense and natural antisense transcripts in the mouse brain. Genomics, 107 (2-3). pp. 88-99. ISSN 0888-7543; ESSN: 1089-8646 http://www.sciencedirect.com/science/article/pii/S0888754316300064 10.1016/j.ygeno.2016.01.006
institution UPM IR
collection UPM IR
language English
description Natural antisense transcripts (NATs) are involved in cellular development and regulatory processes. Multiple NATs at the Sox4 gene locus are spatiotemporally regulated throughout murine cerebral corticogenesis. In the study, we evaluated the potential functional role of Sox4 NATs at Sox4 gene locus. We demonstrated Sox4 sense and NATs formed dsRNA aggregates in the cytoplasm of brain cells. Over expression of Sox4 NATs in NIH/3T3 cells generally did not alter the level of Sox4 mRNA expression or protein translation. Upregulation of a Sox4 NAT known as Sox4ot1 led to the production of a novel small RNA, Sox4_sir3. Its biogenesis is Dicer1-dependent and has characteristics resemble piRNA. Expression of Sox4_sir3 was observed in the marginal and germinative zones of the developing and postnatal brains suggesting a potential role in regulating neurogenesis. We proposed that Sox4 sense-NATs serve as Dicer1-dependent templates to produce a novel endo-siRNA- or piRNA-like Sox4_sir3.
format Article
author Ling, King Hwa
Brautigan, Peter J.
Moore, Sarah
Fraser, Rachel
Cheah, Pike See
Raison, Joy M.
Babic, Milena
Lee, Young Kyung
Daish, Tasman
Mattiske, Deidre M.
Mann, Jeffrey R.
Adelson, David L.
Thomas, Paul Q.
Hahn, Christopher N.
Scott, Hamish S.
spellingShingle Ling, King Hwa
Brautigan, Peter J.
Moore, Sarah
Fraser, Rachel
Cheah, Pike See
Raison, Joy M.
Babic, Milena
Lee, Young Kyung
Daish, Tasman
Mattiske, Deidre M.
Mann, Jeffrey R.
Adelson, David L.
Thomas, Paul Q.
Hahn, Christopher N.
Scott, Hamish S.
Derivation of an endogenous small RNA from double-stranded Sox4 sense and natural antisense transcripts in the mouse brain
author_facet Ling, King Hwa
Brautigan, Peter J.
Moore, Sarah
Fraser, Rachel
Cheah, Pike See
Raison, Joy M.
Babic, Milena
Lee, Young Kyung
Daish, Tasman
Mattiske, Deidre M.
Mann, Jeffrey R.
Adelson, David L.
Thomas, Paul Q.
Hahn, Christopher N.
Scott, Hamish S.
author_sort Ling, King Hwa
title Derivation of an endogenous small RNA from double-stranded Sox4 sense and natural antisense transcripts in the mouse brain
title_short Derivation of an endogenous small RNA from double-stranded Sox4 sense and natural antisense transcripts in the mouse brain
title_full Derivation of an endogenous small RNA from double-stranded Sox4 sense and natural antisense transcripts in the mouse brain
title_fullStr Derivation of an endogenous small RNA from double-stranded Sox4 sense and natural antisense transcripts in the mouse brain
title_full_unstemmed Derivation of an endogenous small RNA from double-stranded Sox4 sense and natural antisense transcripts in the mouse brain
title_sort derivation of an endogenous small rna from double-stranded sox4 sense and natural antisense transcripts in the mouse brain
publisher Elsevier
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/43190/1/Derivation%20of%20an%20endogenous%20small%20RNA%20from%20double-stranded%20Sox4%20sense%20and%20natural%20antisense%20transcripts%20in%20the%20mouse%20brain.pdf
_version_ 1782722018395815936
score 12.935284

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