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Ultrasonic emulsification of parenteral valproic acid-loaded nanoemulsion with response surface methodology and evaluation of its stability

Response surface methodology (RSM) was used to optimize the formulation of a nanoemulsion for central delivery following parenteral administration. A mixture of medium-chain triglyceride (MCT) and safflower seed oil (SSO) was determined as a sole phase from the emulsification properties. Similarly,...

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Main Authors: Tan, Suk Fei, Masoumi, Hamid Reza Fard, Karjiban, Roghayeh Abedi, Stanslas, Johnson, Kirby, Brian P., Basri, Mahiran, Basri, Hamidon
Format: Article
Language:English
Published: Elsevier 2016
Online Access:http://psasir.upm.edu.my/id/eprint/43429/1/Ultrasonic%20emulsification%20of%20parenteral%20valproic%20acid-loaded%20nanoemulsion%20with%20response%20surface%20methodology%20and%20evaluation%20of%20its%20stability.pdf
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spelling oai:psasir.upm.edu.my:43429 http://psasir.upm.edu.my/id/eprint/43429/ Ultrasonic emulsification of parenteral valproic acid-loaded nanoemulsion with response surface methodology and evaluation of its stability Tan, Suk Fei Masoumi, Hamid Reza Fard Karjiban, Roghayeh Abedi Stanslas, Johnson Kirby, Brian P. Basri, Mahiran Basri, Hamidon Response surface methodology (RSM) was used to optimize the formulation of a nanoemulsion for central delivery following parenteral administration. A mixture of medium-chain triglyceride (MCT) and safflower seed oil (SSO) was determined as a sole phase from the emulsification properties. Similarly, a natural surfactant (lecithin) and non-ionic surfactant (Tween 80) (ratio 1:2) were used in the formulation. A central composite design (CCD) with three-factor at five-levels was used to optimize the processing method of high energy ultrasonicator. Effects of pre-sonication ultrasonic intensity (A), sonication time (B), and temperature (C) were studied on the preparation of nanoemulsion loaded with valproic acid. Influence of the aforementioned specifically the effects of the ultrasonic processing parameters on droplet size and polydispersity index were investigated. From the analysis, it was found that the interaction between ultrasonic intensity and sonication time was the most influential factor on the droplet size of nanoemulsion formulated. Ultrasonic intensity (A) significantly affects the polydispersity index value. With this optimization method, a favorable droplet size of a nanoemulsion with reasonable polydispersity index was able to be formulated within a short sonication time. A valproic acid loaded nanoemulsion can be obtained with 60% power intensity for 15 min at 60 °C. Droplet size of 43.21 ± 0.11 nm with polydispersity index of 0.211 were produced. The drug content was then increased to 1.5%. Stability study of nanoemulsion containing 1.5% of valproic acid had a good stability as there are no significant changes in physicochemical aspects such as droplet size and polydispersity index. With the characteristisation study of pH, viscosity, transmission electron microscope (TEM) and stability assessment study the formulated nanoemulsion has the potential to penetrate blood–brain barrier in the treatment of epilepsy. Elsevier 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43429/1/Ultrasonic%20emulsification%20of%20parenteral%20valproic%20acid-loaded%20nanoemulsion%20with%20response%20surface%20methodology%20and%20evaluation%20of%20its%20stability.pdf Tan, Suk Fei and Masoumi, Hamid Reza Fard and Karjiban, Roghayeh Abedi and Stanslas, Johnson and Kirby, Brian P. and Basri, Mahiran and Basri, Hamidon (2016) Ultrasonic emulsification of parenteral valproic acid-loaded nanoemulsion with response surface methodology and evaluation of its stability. Ultrasonics Sonochemistry, 29. pp. 299-308. ISSN 1350-4177; ESSN: 1873-2828 http://www.sciencedirect.com/science/article/pii/S1350417715300377 10.1016/j.ultsonch.2015.09.015
institution UPM IR
collection UPM IR
language English
description Response surface methodology (RSM) was used to optimize the formulation of a nanoemulsion for central delivery following parenteral administration. A mixture of medium-chain triglyceride (MCT) and safflower seed oil (SSO) was determined as a sole phase from the emulsification properties. Similarly, a natural surfactant (lecithin) and non-ionic surfactant (Tween 80) (ratio 1:2) were used in the formulation. A central composite design (CCD) with three-factor at five-levels was used to optimize the processing method of high energy ultrasonicator. Effects of pre-sonication ultrasonic intensity (A), sonication time (B), and temperature (C) were studied on the preparation of nanoemulsion loaded with valproic acid. Influence of the aforementioned specifically the effects of the ultrasonic processing parameters on droplet size and polydispersity index were investigated. From the analysis, it was found that the interaction between ultrasonic intensity and sonication time was the most influential factor on the droplet size of nanoemulsion formulated. Ultrasonic intensity (A) significantly affects the polydispersity index value. With this optimization method, a favorable droplet size of a nanoemulsion with reasonable polydispersity index was able to be formulated within a short sonication time. A valproic acid loaded nanoemulsion can be obtained with 60% power intensity for 15 min at 60 °C. Droplet size of 43.21 ± 0.11 nm with polydispersity index of 0.211 were produced. The drug content was then increased to 1.5%. Stability study of nanoemulsion containing 1.5% of valproic acid had a good stability as there are no significant changes in physicochemical aspects such as droplet size and polydispersity index. With the characteristisation study of pH, viscosity, transmission electron microscope (TEM) and stability assessment study the formulated nanoemulsion has the potential to penetrate blood–brain barrier in the treatment of epilepsy.
format Article
author Tan, Suk Fei
Masoumi, Hamid Reza Fard
Karjiban, Roghayeh Abedi
Stanslas, Johnson
Kirby, Brian P.
Basri, Mahiran
Basri, Hamidon
spellingShingle Tan, Suk Fei
Masoumi, Hamid Reza Fard
Karjiban, Roghayeh Abedi
Stanslas, Johnson
Kirby, Brian P.
Basri, Mahiran
Basri, Hamidon
Ultrasonic emulsification of parenteral valproic acid-loaded nanoemulsion with response surface methodology and evaluation of its stability
author_facet Tan, Suk Fei
Masoumi, Hamid Reza Fard
Karjiban, Roghayeh Abedi
Stanslas, Johnson
Kirby, Brian P.
Basri, Mahiran
Basri, Hamidon
author_sort Tan, Suk Fei
title Ultrasonic emulsification of parenteral valproic acid-loaded nanoemulsion with response surface methodology and evaluation of its stability
title_short Ultrasonic emulsification of parenteral valproic acid-loaded nanoemulsion with response surface methodology and evaluation of its stability
title_full Ultrasonic emulsification of parenteral valproic acid-loaded nanoemulsion with response surface methodology and evaluation of its stability
title_fullStr Ultrasonic emulsification of parenteral valproic acid-loaded nanoemulsion with response surface methodology and evaluation of its stability
title_full_unstemmed Ultrasonic emulsification of parenteral valproic acid-loaded nanoemulsion with response surface methodology and evaluation of its stability
title_sort ultrasonic emulsification of parenteral valproic acid-loaded nanoemulsion with response surface methodology and evaluation of its stability
publisher Elsevier
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/43429/1/Ultrasonic%20emulsification%20of%20parenteral%20valproic%20acid-loaded%20nanoemulsion%20with%20response%20surface%20methodology%20and%20evaluation%20of%20its%20stability.pdf
_version_ 1819296558139572224
score 13.4562235

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