Zinc induces normoxic accumulation of transcriptionally active hypoxia-inducible factor 1-alpha in mammary epithelial cells
Both zinc and the α-subunit of hypoxia-inducible factor (HIF-1α) play important roles in the remodelling of mammary gland tissues. In the present study, we examined the level and the transcriptional activity of HIF-1α in mammary cells upon zinc treatment. In MCF-7 mammary adenocarcinoma and MCF-10A...
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Federation of European Biochemical Societies
2017
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oai:psasir.upm.edu.my:46918 http://psasir.upm.edu.my/id/eprint/46918/ Zinc induces normoxic accumulation of transcriptionally active hypoxia-inducible factor 1-alpha in mammary epithelial cells Lee, S. Y. Mustafa, S. Ching, Y. W. Shafee, Norazizah Both zinc and the α-subunit of hypoxia-inducible factor (HIF-1α) play important roles in the remodelling of mammary gland tissues. In the present study, we examined the level and the transcriptional activity of HIF-1α in mammary cells upon zinc treatment. In MCF-7 mammary adenocarcinoma and MCF-10A mammary epithelial cell lines, the toxicity levels of zinc differ. Interestingly, both cell lines overexpress HIF-1α following zinc treatment. As it was evident from an up-regulation of its specific target gene CA9 that encodes carbonic anhydrase IX, the stabilized HIF-1α translocated to the nucleus and was transcriptionally active. Hence, we conclude that zinc causes normoxic accumulation of transcriptionally active HIF-1α by interfering with its post-translational regulation. Federation of European Biochemical Societies 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46918/1/Zinc%20induces%20normoxic%20accumulation%20of%20transcriptionally%20active%20hypoxia-inducible%20factor%201-alpha%20in%20mammary%20epithelial%20cells.pdf Lee, S. Y. and Mustafa, S. and Ching, Y. W. and Shafee, Norazizah (2017) Zinc induces normoxic accumulation of transcriptionally active hypoxia-inducible factor 1-alpha in mammary epithelial cells. FEBS Letters, 51. pp. 89-95. ISSN 0014-5793; ESSN: 1873-3468 http://molecbio.ru/?view=article&id=3830 10.7868/S0026898417010116 |
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English |
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Both zinc and the α-subunit of hypoxia-inducible factor (HIF-1α) play important roles in the remodelling of mammary gland tissues. In the present study, we examined the level and the transcriptional activity of HIF-1α in mammary cells upon zinc treatment. In MCF-7 mammary adenocarcinoma and MCF-10A mammary epithelial cell lines, the toxicity levels of zinc differ. Interestingly, both cell lines overexpress HIF-1α following zinc treatment. As it was evident from an up-regulation of its specific target gene CA9 that encodes carbonic anhydrase IX, the stabilized HIF-1α translocated to the nucleus and was transcriptionally active. Hence, we conclude that zinc causes normoxic accumulation of transcriptionally active HIF-1α by interfering with its post-translational regulation. |
| format |
Article |
| author |
Lee, S. Y. Mustafa, S. Ching, Y. W. Shafee, Norazizah |
| spellingShingle |
Lee, S. Y. Mustafa, S. Ching, Y. W. Shafee, Norazizah Zinc induces normoxic accumulation of transcriptionally active hypoxia-inducible factor 1-alpha in mammary epithelial cells |
| author_facet |
Lee, S. Y. Mustafa, S. Ching, Y. W. Shafee, Norazizah |
| author_sort |
Lee, S. Y. |
| title |
Zinc induces normoxic accumulation of transcriptionally active hypoxia-inducible factor 1-alpha in mammary epithelial cells |
| title_short |
Zinc induces normoxic accumulation of transcriptionally active hypoxia-inducible factor 1-alpha in mammary epithelial cells |
| title_full |
Zinc induces normoxic accumulation of transcriptionally active hypoxia-inducible factor 1-alpha in mammary epithelial cells |
| title_fullStr |
Zinc induces normoxic accumulation of transcriptionally active hypoxia-inducible factor 1-alpha in mammary epithelial cells |
| title_full_unstemmed |
Zinc induces normoxic accumulation of transcriptionally active hypoxia-inducible factor 1-alpha in mammary epithelial cells |
| title_sort |
zinc induces normoxic accumulation of transcriptionally active hypoxia-inducible factor 1-alpha in mammary epithelial cells |
| publisher |
Federation of European Biochemical Societies |
| publishDate |
2017 |
| url |
http://psasir.upm.edu.my/id/eprint/46918/1/Zinc%20induces%20normoxic%20accumulation%20of%20transcriptionally%20active%20hypoxia-inducible%20factor%201-alpha%20in%20mammary%20epithelial%20cells.pdf |
| _version_ |
1819296760819875840 |
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13.4562235 |